Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3078 and Pf6N2E2_3079 are separated by 174 nucleotides Pf6N2E2_3079 and Pf6N2E2_3080 overlap by 4 nucleotides Pf6N2E2_3080 and Pf6N2E2_3081 are separated by 126 nucleotides
Pf6N2E2_3078 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 3,566,203 to 3,567,237
_3078
Pf6N2E2_3079 - Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA, at 3,567,412 to 3,568,617
_3079
Pf6N2E2_3080 - Cysteine desulfurase CsdA-CsdE, sulfur acceptor protein CsdE, at 3,568,614 to 3,569,021
_3080
Pf6N2E2_3081 - HesA/MoeB/ThiF family protein related to EC-YgdL, at 3,569,148 to 3,569,966
_3081
Position (kb)
3567
3568
3569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3567.235 kb on + strand at 3567.235 kb on + strand at 3567.235 kb on + strand at 3567.235 kb on + strand at 3567.235 kb on + strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.389 kb on + strand at 3567.390 kb on - strand at 3567.481 kb on + strand at 3567.482 kb on - strand at 3567.482 kb on - strand at 3567.541 kb on + strand, within Pf6N2E2_3079 at 3567.541 kb on + strand, within Pf6N2E2_3079 at 3567.541 kb on + strand, within Pf6N2E2_3079 at 3567.541 kb on + strand, within Pf6N2E2_3079 at 3567.542 kb on - strand, within Pf6N2E2_3079 at 3567.542 kb on - strand, within Pf6N2E2_3079 at 3567.542 kb on - strand, within Pf6N2E2_3079 at 3567.542 kb on - strand, within Pf6N2E2_3079 at 3567.544 kb on + strand, within Pf6N2E2_3079 at 3567.544 kb on + strand, within Pf6N2E2_3079 at 3567.545 kb on - strand, within Pf6N2E2_3079 at 3567.728 kb on + strand, within Pf6N2E2_3079 at 3567.729 kb on - strand, within Pf6N2E2_3079 at 3567.729 kb on - strand, within Pf6N2E2_3079 at 3567.921 kb on - strand, within Pf6N2E2_3079 at 3568.060 kb on + strand, within Pf6N2E2_3079 at 3568.061 kb on - strand, within Pf6N2E2_3079 at 3568.277 kb on - strand, within Pf6N2E2_3079 at 3568.277 kb on - strand, within Pf6N2E2_3079 at 3568.333 kb on + strand, within Pf6N2E2_3079 at 3568.333 kb on + strand, within Pf6N2E2_3079 at 3568.333 kb on + strand, within Pf6N2E2_3079 at 3568.334 kb on - strand, within Pf6N2E2_3079 at 3568.549 kb on + strand at 3568.549 kb on + strand at 3568.549 kb on + strand at 3568.549 kb on + strand at 3568.968 kb on - strand, within Pf6N2E2_3080 at 3569.201 kb on + strand at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,567,235 + -0.1 3,567,235 + +0.0 3,567,235 + -0.6 3,567,235 + -0.3 3,567,235 + +0.3 3,567,236 - -1.0 3,567,236 - +0.3 3,567,236 - -1.7 3,567,236 - +1.5 3,567,236 - -1.6 3,567,236 - +2.0 3,567,389 + +0.4 3,567,390 - -0.8 3,567,481 + -1.5 3,567,482 - -0.4 3,567,482 - -1.3 3,567,541 + Pf6N2E2_3079 0.11 -0.2 3,567,541 + Pf6N2E2_3079 0.11 +0.1 3,567,541 + Pf6N2E2_3079 0.11 -0.5 3,567,541 + Pf6N2E2_3079 0.11 +0.0 3,567,542 - Pf6N2E2_3079 0.11 -0.5 3,567,542 - Pf6N2E2_3079 0.11 +0.1 3,567,542 - Pf6N2E2_3079 0.11 -0.9 3,567,542 - Pf6N2E2_3079 0.11 -0.8 3,567,544 + Pf6N2E2_3079 0.11 -1.6 3,567,544 + Pf6N2E2_3079 0.11 -1.6 3,567,545 - Pf6N2E2_3079 0.11 +1.1 3,567,728 + Pf6N2E2_3079 0.26 +1.4 3,567,729 - Pf6N2E2_3079 0.26 -1.1 3,567,729 - Pf6N2E2_3079 0.26 -0.9 3,567,921 - Pf6N2E2_3079 0.42 -1.7 3,568,060 + Pf6N2E2_3079 0.54 -0.7 3,568,061 - Pf6N2E2_3079 0.54 -0.5 3,568,277 - Pf6N2E2_3079 0.72 -1.3 3,568,277 - Pf6N2E2_3079 0.72 -0.1 3,568,333 + Pf6N2E2_3079 0.76 -0.8 3,568,333 + Pf6N2E2_3079 0.76 +0.4 3,568,333 + Pf6N2E2_3079 0.76 -3.2 3,568,334 - Pf6N2E2_3079 0.76 -1.1 3,568,549 + -0.8 3,568,549 + -2.2 3,568,549 + -1.1 3,568,549 + +0.7 3,568,968 - Pf6N2E2_3080 0.87 -0.9 3,569,201 + -1.3 3,569,380 + Pf6N2E2_3081 0.28 -0.6 3,569,380 + Pf6N2E2_3081 0.28 -1.0 3,569,381 - Pf6N2E2_3081 0.28 +0.3 3,569,381 - Pf6N2E2_3081 0.28 -0.5 3,569,433 + Pf6N2E2_3081 0.35 +0.1 3,569,433 + Pf6N2E2_3081 0.35 -0.5 3,569,433 + Pf6N2E2_3081 0.35 +0.4 3,569,434 - Pf6N2E2_3081 0.35 -0.6 3,569,434 - Pf6N2E2_3081 0.35 -0.8
Or see this region's nucleotide sequence