Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3079

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3078 and Pf6N2E2_3079 are separated by 174 nucleotidesPf6N2E2_3079 and Pf6N2E2_3080 overlap by 4 nucleotidesPf6N2E2_3080 and Pf6N2E2_3081 are separated by 126 nucleotides Pf6N2E2_3078 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 3,566,203 to 3,567,237 _3078 Pf6N2E2_3079 - Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA, at 3,567,412 to 3,568,617 _3079 Pf6N2E2_3080 - Cysteine desulfurase CsdA-CsdE, sulfur acceptor protein CsdE, at 3,568,614 to 3,569,021 _3080 Pf6N2E2_3081 - HesA/MoeB/ThiF family protein related to EC-YgdL, at 3,569,148 to 3,569,966 _3081 Position (kb) 3567 3568 3569Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3567.235 kb on + strandat 3567.235 kb on + strandat 3567.235 kb on + strandat 3567.235 kb on + strandat 3567.235 kb on + strandat 3567.236 kb on - strandat 3567.236 kb on - strandat 3567.236 kb on - strandat 3567.236 kb on - strandat 3567.236 kb on - strandat 3567.236 kb on - strandat 3567.389 kb on + strandat 3567.390 kb on - strandat 3567.481 kb on + strandat 3567.482 kb on - strandat 3567.482 kb on - strandat 3567.541 kb on + strand, within Pf6N2E2_3079at 3567.541 kb on + strand, within Pf6N2E2_3079at 3567.541 kb on + strand, within Pf6N2E2_3079at 3567.541 kb on + strand, within Pf6N2E2_3079at 3567.542 kb on - strand, within Pf6N2E2_3079at 3567.542 kb on - strand, within Pf6N2E2_3079at 3567.542 kb on - strand, within Pf6N2E2_3079at 3567.542 kb on - strand, within Pf6N2E2_3079at 3567.544 kb on + strand, within Pf6N2E2_3079at 3567.544 kb on + strand, within Pf6N2E2_3079at 3567.545 kb on - strand, within Pf6N2E2_3079at 3567.728 kb on + strand, within Pf6N2E2_3079at 3567.729 kb on - strand, within Pf6N2E2_3079at 3567.729 kb on - strand, within Pf6N2E2_3079at 3567.921 kb on - strand, within Pf6N2E2_3079at 3568.060 kb on + strand, within Pf6N2E2_3079at 3568.061 kb on - strand, within Pf6N2E2_3079at 3568.277 kb on - strand, within Pf6N2E2_3079at 3568.277 kb on - strand, within Pf6N2E2_3079at 3568.333 kb on + strand, within Pf6N2E2_3079at 3568.333 kb on + strand, within Pf6N2E2_3079at 3568.333 kb on + strand, within Pf6N2E2_3079at 3568.334 kb on - strand, within Pf6N2E2_3079at 3568.549 kb on + strandat 3568.549 kb on + strandat 3568.549 kb on + strandat 3568.549 kb on + strandat 3568.968 kb on - strand, within Pf6N2E2_3080at 3569.201 kb on + strandat 3569.380 kb on + strand, within Pf6N2E2_3081at 3569.380 kb on + strand, within Pf6N2E2_3081at 3569.381 kb on - strand, within Pf6N2E2_3081at 3569.381 kb on - strand, within Pf6N2E2_3081at 3569.433 kb on + strand, within Pf6N2E2_3081at 3569.433 kb on + strand, within Pf6N2E2_3081at 3569.433 kb on + strand, within Pf6N2E2_3081at 3569.434 kb on - strand, within Pf6N2E2_3081at 3569.434 kb on - strand, within Pf6N2E2_3081

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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3,567,235 + -0.1
3,567,235 + +0.0
3,567,235 + -0.6
3,567,235 + -0.3
3,567,235 + +0.3
3,567,236 - -1.0
3,567,236 - +0.3
3,567,236 - -1.7
3,567,236 - +1.5
3,567,236 - -1.6
3,567,236 - +2.0
3,567,389 + +0.4
3,567,390 - -0.8
3,567,481 + -1.5
3,567,482 - -0.4
3,567,482 - -1.3
3,567,541 + Pf6N2E2_3079 0.11 -0.2
3,567,541 + Pf6N2E2_3079 0.11 +0.1
3,567,541 + Pf6N2E2_3079 0.11 -0.5
3,567,541 + Pf6N2E2_3079 0.11 +0.0
3,567,542 - Pf6N2E2_3079 0.11 -0.5
3,567,542 - Pf6N2E2_3079 0.11 +0.1
3,567,542 - Pf6N2E2_3079 0.11 -0.9
3,567,542 - Pf6N2E2_3079 0.11 -0.8
3,567,544 + Pf6N2E2_3079 0.11 -1.6
3,567,544 + Pf6N2E2_3079 0.11 -1.6
3,567,545 - Pf6N2E2_3079 0.11 +1.1
3,567,728 + Pf6N2E2_3079 0.26 +1.4
3,567,729 - Pf6N2E2_3079 0.26 -1.1
3,567,729 - Pf6N2E2_3079 0.26 -0.9
3,567,921 - Pf6N2E2_3079 0.42 -1.7
3,568,060 + Pf6N2E2_3079 0.54 -0.7
3,568,061 - Pf6N2E2_3079 0.54 -0.5
3,568,277 - Pf6N2E2_3079 0.72 -1.3
3,568,277 - Pf6N2E2_3079 0.72 -0.1
3,568,333 + Pf6N2E2_3079 0.76 -0.8
3,568,333 + Pf6N2E2_3079 0.76 +0.4
3,568,333 + Pf6N2E2_3079 0.76 -3.2
3,568,334 - Pf6N2E2_3079 0.76 -1.1
3,568,549 + -0.8
3,568,549 + -2.2
3,568,549 + -1.1
3,568,549 + +0.7
3,568,968 - Pf6N2E2_3080 0.87 -0.9
3,569,201 + -1.3
3,569,380 + Pf6N2E2_3081 0.28 -0.6
3,569,380 + Pf6N2E2_3081 0.28 -1.0
3,569,381 - Pf6N2E2_3081 0.28 +0.3
3,569,381 - Pf6N2E2_3081 0.28 -0.5
3,569,433 + Pf6N2E2_3081 0.35 +0.1
3,569,433 + Pf6N2E2_3081 0.35 -0.5
3,569,433 + Pf6N2E2_3081 0.35 +0.4
3,569,434 - Pf6N2E2_3081 0.35 -0.6
3,569,434 - Pf6N2E2_3081 0.35 -0.8

Or see this region's nucleotide sequence