Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2748 and Pf6N2E2_2749 are separated by 10 nucleotides Pf6N2E2_2749 and Pf6N2E2_2750 are separated by 129 nucleotides Pf6N2E2_2750 and Pf6N2E2_2751 are separated by 29 nucleotides Pf6N2E2_2751 and Pf6N2E2_2752 are separated by 98 nucleotides
Pf6N2E2_2748 - Glycine cleavage system transcriptional antiactivator GcvR, at 3,240,881 to 3,241,441
_2748
Pf6N2E2_2749 - Thiol peroxidase, Bcp-type (EC 1.11.1.15), at 3,241,452 to 3,241,925
_2749
Pf6N2E2_2750 - Putative permease PerM (= YfgO), at 3,242,055 to 3,243,125
_2750
Pf6N2E2_2751 - Rhodanese-like domain protein, at 3,243,155 to 3,243,394
_2751
Pf6N2E2_2752 - Exported zinc metalloprotease YfgC precursor, at 3,243,493 to 3,244,926
_2752
Position (kb)
3242
3243
3244 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3241.172 kb on + strand, within Pf6N2E2_2748 at 3241.172 kb on + strand, within Pf6N2E2_2748 at 3241.173 kb on - strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.555 kb on - strand, within Pf6N2E2_2749 at 3241.555 kb on - strand, within Pf6N2E2_2749 at 3241.555 kb on - strand, within Pf6N2E2_2749 at 3241.560 kb on + strand, within Pf6N2E2_2749 at 3241.561 kb on - strand, within Pf6N2E2_2749 at 3241.561 kb on - strand, within Pf6N2E2_2749 at 3241.561 kb on - strand, within Pf6N2E2_2749 at 3241.618 kb on - strand, within Pf6N2E2_2749 at 3241.618 kb on - strand, within Pf6N2E2_2749 at 3241.794 kb on + strand, within Pf6N2E2_2749 at 3241.794 kb on + strand, within Pf6N2E2_2749 at 3241.795 kb on - strand, within Pf6N2E2_2749 at 3241.795 kb on - strand, within Pf6N2E2_2749 at 3241.795 kb on - strand, within Pf6N2E2_2749 at 3241.941 kb on + strand at 3241.994 kb on + strand at 3242.056 kb on + strand at 3242.057 kb on - strand at 3242.057 kb on - strand at 3242.253 kb on + strand, within Pf6N2E2_2750 at 3242.253 kb on + strand, within Pf6N2E2_2750 at 3242.253 kb on + strand, within Pf6N2E2_2750 at 3242.254 kb on - strand, within Pf6N2E2_2750 at 3242.254 kb on - strand, within Pf6N2E2_2750 at 3242.254 kb on - strand, within Pf6N2E2_2750 at 3242.287 kb on + strand, within Pf6N2E2_2750 at 3242.288 kb on - strand, within Pf6N2E2_2750 at 3242.365 kb on + strand, within Pf6N2E2_2750 at 3242.413 kb on + strand, within Pf6N2E2_2750 at 3242.443 kb on + strand, within Pf6N2E2_2750 at 3242.443 kb on + strand, within Pf6N2E2_2750 at 3242.443 kb on + strand, within Pf6N2E2_2750 at 3242.444 kb on - strand, within Pf6N2E2_2750 at 3242.444 kb on - strand, within Pf6N2E2_2750 at 3242.444 kb on - strand, within Pf6N2E2_2750 at 3242.444 kb on - strand, within Pf6N2E2_2750 at 3242.444 kb on - strand, within Pf6N2E2_2750 at 3242.444 kb on - strand, within Pf6N2E2_2750 at 3242.444 kb on - strand, within Pf6N2E2_2750 at 3242.491 kb on + strand, within Pf6N2E2_2750 at 3242.492 kb on - strand, within Pf6N2E2_2750 at 3242.632 kb on + strand, within Pf6N2E2_2750 at 3242.633 kb on - strand, within Pf6N2E2_2750 at 3242.633 kb on - strand, within Pf6N2E2_2750 at 3242.633 kb on - strand, within Pf6N2E2_2750 at 3242.872 kb on - strand, within Pf6N2E2_2750 at 3242.977 kb on + strand, within Pf6N2E2_2750 at 3243.092 kb on - strand at 3243.092 kb on - strand at 3243.380 kb on + strand at 3243.380 kb on + strand at 3243.427 kb on + strand at 3243.428 kb on - strand at 3243.432 kb on + strand at 3243.432 kb on + strand at 3243.432 kb on + strand at 3243.432 kb on + strand at 3243.433 kb on - strand at 3243.433 kb on - strand at 3243.433 kb on - strand at 3243.459 kb on + strand at 3243.464 kb on + strand at 3243.464 kb on + strand at 3243.464 kb on + strand at 3243.464 kb on + strand at 3243.465 kb on - strand at 3243.481 kb on - strand at 3243.688 kb on + strand, within Pf6N2E2_2752 at 3243.688 kb on + strand, within Pf6N2E2_2752 at 3243.689 kb on - strand, within Pf6N2E2_2752 at 3243.706 kb on + strand, within Pf6N2E2_2752 at 3243.706 kb on + strand, within Pf6N2E2_2752 at 3243.707 kb on - strand, within Pf6N2E2_2752 at 3243.853 kb on + strand, within Pf6N2E2_2752 at 3243.854 kb on - strand, within Pf6N2E2_2752 at 3244.117 kb on + strand, within Pf6N2E2_2752
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,241,172 + Pf6N2E2_2748 0.52 +0.0 3,241,172 + Pf6N2E2_2748 0.52 +3.0 3,241,173 - Pf6N2E2_2748 0.52 +1.0 3,241,181 + Pf6N2E2_2748 0.53 -1.3 3,241,181 + Pf6N2E2_2748 0.53 +0.7 3,241,181 + Pf6N2E2_2748 0.53 -1.0 3,241,181 + Pf6N2E2_2748 0.53 -0.1 3,241,182 - Pf6N2E2_2748 0.54 +0.6 3,241,182 - Pf6N2E2_2748 0.54 +2.0 3,241,182 - Pf6N2E2_2748 0.54 +1.6 3,241,182 - Pf6N2E2_2748 0.54 +0.4 3,241,182 - Pf6N2E2_2748 0.54 -0.1 3,241,182 - Pf6N2E2_2748 0.54 +0.7 3,241,277 + Pf6N2E2_2748 0.71 -0.6 3,241,277 + Pf6N2E2_2748 0.71 +1.0 3,241,277 + Pf6N2E2_2748 0.71 -0.1 3,241,277 + Pf6N2E2_2748 0.71 -2.2 3,241,277 + Pf6N2E2_2748 0.71 +0.2 3,241,277 + Pf6N2E2_2748 0.71 +0.1 3,241,278 - Pf6N2E2_2748 0.71 -0.1 3,241,278 - Pf6N2E2_2748 0.71 +0.1 3,241,278 - Pf6N2E2_2748 0.71 +1.4 3,241,278 - Pf6N2E2_2748 0.71 +0.0 3,241,278 - Pf6N2E2_2748 0.71 +0.2 3,241,278 - Pf6N2E2_2748 0.71 +1.0 3,241,439 + -1.3 3,241,439 + -0.0 3,241,439 + +1.2 3,241,439 + -0.9 3,241,439 + -0.7 3,241,440 - +0.3 3,241,440 - -0.6 3,241,440 - -0.2 3,241,440 - +0.9 3,241,440 - +0.0 3,241,554 + Pf6N2E2_2749 0.22 -0.3 3,241,554 + Pf6N2E2_2749 0.22 -0.2 3,241,554 + Pf6N2E2_2749 0.22 +0.2 3,241,554 + Pf6N2E2_2749 0.22 +0.6 3,241,554 + Pf6N2E2_2749 0.22 +1.2 3,241,555 - Pf6N2E2_2749 0.22 -1.0 3,241,555 - Pf6N2E2_2749 0.22 +1.6 3,241,555 - Pf6N2E2_2749 0.22 -0.1 3,241,560 + Pf6N2E2_2749 0.23 +1.6 3,241,561 - Pf6N2E2_2749 0.23 -0.1 3,241,561 - Pf6N2E2_2749 0.23 +0.4 3,241,561 - Pf6N2E2_2749 0.23 +0.6 3,241,618 - Pf6N2E2_2749 0.35 -0.8 3,241,618 - Pf6N2E2_2749 0.35 +0.4 3,241,794 + Pf6N2E2_2749 0.72 -0.4 3,241,794 + Pf6N2E2_2749 0.72 -1.0 3,241,795 - Pf6N2E2_2749 0.72 +1.2 3,241,795 - Pf6N2E2_2749 0.72 -0.2 3,241,795 - Pf6N2E2_2749 0.72 +1.0 3,241,941 + -0.1 3,241,994 + +0.0 3,242,056 + -2.2 3,242,057 - +0.6 3,242,057 - -0.1 3,242,253 + Pf6N2E2_2750 0.18 -1.6 3,242,253 + Pf6N2E2_2750 0.18 +0.0 3,242,253 + Pf6N2E2_2750 0.18 +1.0 3,242,254 - Pf6N2E2_2750 0.19 -1.3 3,242,254 - Pf6N2E2_2750 0.19 -0.5 3,242,254 - Pf6N2E2_2750 0.19 -0.6 3,242,287 + Pf6N2E2_2750 0.22 +0.2 3,242,288 - Pf6N2E2_2750 0.22 -0.8 3,242,365 + Pf6N2E2_2750 0.29 -2.0 3,242,413 + Pf6N2E2_2750 0.33 +2.0 3,242,443 + Pf6N2E2_2750 0.36 +1.0 3,242,443 + Pf6N2E2_2750 0.36 +0.3 3,242,443 + Pf6N2E2_2750 0.36 -1.3 3,242,444 - Pf6N2E2_2750 0.36 -1.4 3,242,444 - Pf6N2E2_2750 0.36 +0.2 3,242,444 - Pf6N2E2_2750 0.36 +1.0 3,242,444 - Pf6N2E2_2750 0.36 +0.0 3,242,444 - Pf6N2E2_2750 0.36 -1.0 3,242,444 - Pf6N2E2_2750 0.36 -0.6 3,242,444 - Pf6N2E2_2750 0.36 -2.0 3,242,491 + Pf6N2E2_2750 0.41 +1.7 3,242,492 - Pf6N2E2_2750 0.41 -0.2 3,242,632 + Pf6N2E2_2750 0.54 +1.0 3,242,633 - Pf6N2E2_2750 0.54 -1.0 3,242,633 - Pf6N2E2_2750 0.54 +0.6 3,242,633 - Pf6N2E2_2750 0.54 -0.6 3,242,872 - Pf6N2E2_2750 0.76 -0.6 3,242,977 + Pf6N2E2_2750 0.86 -1.0 3,243,092 - -1.2 3,243,092 - +0.0 3,243,380 + +1.0 3,243,380 + +0.8 3,243,427 + -0.1 3,243,428 - -0.6 3,243,432 + -0.3 3,243,432 + -1.4 3,243,432 + +0.6 3,243,432 + +0.0 3,243,433 - +0.6 3,243,433 - +0.6 3,243,433 - -0.4 3,243,459 + +1.0 3,243,464 + -0.3 3,243,464 + +0.2 3,243,464 + -1.0 3,243,464 + +2.0 3,243,465 - +0.7 3,243,481 - -0.2 3,243,688 + Pf6N2E2_2752 0.14 +1.0 3,243,688 + Pf6N2E2_2752 0.14 +1.0 3,243,689 - Pf6N2E2_2752 0.14 -1.3 3,243,706 + Pf6N2E2_2752 0.15 +1.0 3,243,706 + Pf6N2E2_2752 0.15 +1.0 3,243,707 - Pf6N2E2_2752 0.15 +0.4 3,243,853 + Pf6N2E2_2752 0.25 +1.0 3,243,854 - Pf6N2E2_2752 0.25 +0.3 3,244,117 + Pf6N2E2_2752 0.44 +1.0
Or see this region's nucleotide sequence