Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2730 and Pf6N2E2_2731 overlap by 39 nucleotides Pf6N2E2_2731 and Pf6N2E2_2732 are separated by 440 nucleotides Pf6N2E2_2732 and Pf6N2E2_2733 are separated by 3 nucleotides Pf6N2E2_2733 and Pf6N2E2_2734 are separated by 103 nucleotides
Pf6N2E2_2730 - glutamine synthetase family protein, at 3,222,083 to 3,223,465
_2730
Pf6N2E2_2731 - hypothetical protein, at 3,223,427 to 3,223,546
_2731
Pf6N2E2_2732 - Transcriptional regulator, LysR family, at 3,223,987 to 3,224,919
_2732
Pf6N2E2_2733 - Probable 5-carboxymethyl-2-hydroxymuconate delta isomerase, at 3,224,923 to 3,225,288
_2733
Pf6N2E2_2734 - Transcriptional regulator, LysR family, at 3,225,392 to 3,226,315
_2734
Position (kb)
3223
3224
3225 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3223.011 kb on + strand, within Pf6N2E2_2730 at 3223.011 kb on + strand, within Pf6N2E2_2730 at 3223.012 kb on - strand, within Pf6N2E2_2730 at 3223.041 kb on + strand, within Pf6N2E2_2730 at 3223.041 kb on + strand, within Pf6N2E2_2730 at 3223.044 kb on + strand, within Pf6N2E2_2730 at 3223.044 kb on + strand, within Pf6N2E2_2730 at 3223.044 kb on + strand, within Pf6N2E2_2730 at 3223.058 kb on + strand, within Pf6N2E2_2730 at 3223.086 kb on + strand, within Pf6N2E2_2730 at 3223.174 kb on + strand, within Pf6N2E2_2730 at 3223.174 kb on + strand, within Pf6N2E2_2730 at 3223.175 kb on - strand, within Pf6N2E2_2730 at 3223.175 kb on - strand, within Pf6N2E2_2730 at 3223.175 kb on - strand, within Pf6N2E2_2730 at 3223.175 kb on - strand, within Pf6N2E2_2730 at 3223.175 kb on - strand, within Pf6N2E2_2730 at 3223.201 kb on - strand, within Pf6N2E2_2730 at 3223.285 kb on + strand, within Pf6N2E2_2730 at 3223.478 kb on - strand, within Pf6N2E2_2731 at 3223.555 kb on + strand at 3223.555 kb on + strand at 3223.555 kb on + strand at 3223.555 kb on + strand at 3223.555 kb on + strand at 3223.556 kb on - strand at 3223.556 kb on - strand at 3223.556 kb on - strand at 3223.556 kb on - strand at 3223.556 kb on - strand at 3223.556 kb on - strand at 3223.558 kb on + strand at 3223.558 kb on + strand at 3223.559 kb on - strand at 3223.564 kb on + strand at 3223.565 kb on - strand at 3223.565 kb on - strand at 3223.565 kb on - strand at 3223.811 kb on + strand at 3223.811 kb on + strand at 3223.812 kb on - strand at 3223.817 kb on + strand at 3223.858 kb on + strand at 3223.859 kb on - strand at 3223.859 kb on - strand at 3223.863 kb on + strand at 3223.863 kb on + strand at 3223.863 kb on + strand at 3223.863 kb on + strand at 3223.863 kb on + strand at 3223.863 kb on + strand at 3223.864 kb on - strand at 3223.864 kb on - strand at 3223.864 kb on - strand at 3224.113 kb on + strand, within Pf6N2E2_2732 at 3224.114 kb on - strand, within Pf6N2E2_2732 at 3224.154 kb on - strand, within Pf6N2E2_2732 at 3224.154 kb on - strand, within Pf6N2E2_2732 at 3224.188 kb on + strand, within Pf6N2E2_2732 at 3224.188 kb on + strand, within Pf6N2E2_2732 at 3224.188 kb on + strand, within Pf6N2E2_2732 at 3224.188 kb on + strand, within Pf6N2E2_2732 at 3224.189 kb on - strand, within Pf6N2E2_2732 at 3224.189 kb on - strand, within Pf6N2E2_2732 at 3224.189 kb on - strand, within Pf6N2E2_2732 at 3224.206 kb on + strand, within Pf6N2E2_2732 at 3224.207 kb on - strand, within Pf6N2E2_2732 at 3224.207 kb on - strand, within Pf6N2E2_2732 at 3224.298 kb on + strand, within Pf6N2E2_2732 at 3224.299 kb on - strand, within Pf6N2E2_2732 at 3224.315 kb on - strand, within Pf6N2E2_2732 at 3224.692 kb on + strand, within Pf6N2E2_2732 at 3224.692 kb on + strand, within Pf6N2E2_2732 at 3224.693 kb on - strand, within Pf6N2E2_2732 at 3224.693 kb on - strand, within Pf6N2E2_2732 at 3224.693 kb on - strand, within Pf6N2E2_2732 at 3224.693 kb on - strand, within Pf6N2E2_2732 at 3224.949 kb on - strand at 3225.266 kb on + strand at 3225.266 kb on + strand at 3225.266 kb on + strand at 3225.266 kb on + strand at 3225.267 kb on - strand at 3225.267 kb on - strand at 3225.267 kb on - strand at 3225.311 kb on + strand at 3225.312 kb on - strand at 3225.342 kb on + strand at 3225.353 kb on + strand at 3225.401 kb on + strand at 3225.401 kb on + strand at 3225.572 kb on - strand, within Pf6N2E2_2734 at 3225.573 kb on + strand, within Pf6N2E2_2734 at 3225.574 kb on - strand, within Pf6N2E2_2734 at 3225.744 kb on + strand, within Pf6N2E2_2734 at 3225.745 kb on - strand, within Pf6N2E2_2734
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,223,011 + Pf6N2E2_2730 0.67 +0.1 3,223,011 + Pf6N2E2_2730 0.67 +0.3 3,223,012 - Pf6N2E2_2730 0.67 +1.9 3,223,041 + Pf6N2E2_2730 0.69 +1.1 3,223,041 + Pf6N2E2_2730 0.69 -2.6 3,223,044 + Pf6N2E2_2730 0.69 +0.2 3,223,044 + Pf6N2E2_2730 0.69 +0.3 3,223,044 + Pf6N2E2_2730 0.69 +1.3 3,223,058 + Pf6N2E2_2730 0.70 +0.6 3,223,086 + Pf6N2E2_2730 0.73 +0.5 3,223,174 + Pf6N2E2_2730 0.79 +0.9 3,223,174 + Pf6N2E2_2730 0.79 +1.6 3,223,175 - Pf6N2E2_2730 0.79 +1.0 3,223,175 - Pf6N2E2_2730 0.79 +0.3 3,223,175 - Pf6N2E2_2730 0.79 +0.3 3,223,175 - Pf6N2E2_2730 0.79 +1.1 3,223,175 - Pf6N2E2_2730 0.79 +0.3 3,223,201 - Pf6N2E2_2730 0.81 +0.2 3,223,285 + Pf6N2E2_2730 0.87 +3.6 3,223,478 - Pf6N2E2_2731 0.42 -0.1 3,223,555 + +1.0 3,223,555 + +0.0 3,223,555 + +0.6 3,223,555 + +1.4 3,223,555 + +1.2 3,223,556 - -0.6 3,223,556 - +1.1 3,223,556 - +0.2 3,223,556 - +2.0 3,223,556 - +1.6 3,223,556 - +0.5 3,223,558 + +2.2 3,223,558 + -0.4 3,223,559 - +1.0 3,223,564 + +1.3 3,223,565 - -0.3 3,223,565 - -0.1 3,223,565 - +0.6 3,223,811 + +1.5 3,223,811 + +1.0 3,223,812 - -0.9 3,223,817 + +1.4 3,223,858 + +1.0 3,223,859 - -1.5 3,223,859 - -0.9 3,223,863 + +2.6 3,223,863 + +0.2 3,223,863 + +0.9 3,223,863 + +0.9 3,223,863 + +1.3 3,223,863 + +0.0 3,223,864 - -1.2 3,223,864 - +1.0 3,223,864 - -0.3 3,224,113 + Pf6N2E2_2732 0.14 +1.7 3,224,114 - Pf6N2E2_2732 0.14 -0.2 3,224,154 - Pf6N2E2_2732 0.18 -0.8 3,224,154 - Pf6N2E2_2732 0.18 -1.6 3,224,188 + Pf6N2E2_2732 0.22 +0.3 3,224,188 + Pf6N2E2_2732 0.22 +0.0 3,224,188 + Pf6N2E2_2732 0.22 +1.0 3,224,188 + Pf6N2E2_2732 0.22 -2.0 3,224,189 - Pf6N2E2_2732 0.22 -1.5 3,224,189 - Pf6N2E2_2732 0.22 -1.4 3,224,189 - Pf6N2E2_2732 0.22 +1.6 3,224,206 + Pf6N2E2_2732 0.23 -1.3 3,224,207 - Pf6N2E2_2732 0.24 -0.8 3,224,207 - Pf6N2E2_2732 0.24 -0.6 3,224,298 + Pf6N2E2_2732 0.33 -0.4 3,224,299 - Pf6N2E2_2732 0.33 +0.5 3,224,315 - Pf6N2E2_2732 0.35 +0.0 3,224,692 + Pf6N2E2_2732 0.76 -0.6 3,224,692 + Pf6N2E2_2732 0.76 -1.0 3,224,693 - Pf6N2E2_2732 0.76 +1.0 3,224,693 - Pf6N2E2_2732 0.76 -0.3 3,224,693 - Pf6N2E2_2732 0.76 +0.0 3,224,693 - Pf6N2E2_2732 0.76 +1.0 3,224,949 - +3.3 3,225,266 + -1.3 3,225,266 + +2.6 3,225,266 + +0.0 3,225,266 + -0.6 3,225,267 - -1.0 3,225,267 - -1.6 3,225,267 - -1.0 3,225,311 + +1.0 3,225,312 - +0.0 3,225,342 + +0.4 3,225,353 + +1.0 3,225,401 + -1.3 3,225,401 + +1.2 3,225,572 - Pf6N2E2_2734 0.19 -0.3 3,225,573 + Pf6N2E2_2734 0.20 +1.6 3,225,574 - Pf6N2E2_2734 0.20 +0.4 3,225,744 + Pf6N2E2_2734 0.38 +1.6 3,225,745 - Pf6N2E2_2734 0.38 -1.1
Or see this region's nucleotide sequence