Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2177 and Pf6N2E2_2178 overlap by 17 nucleotides Pf6N2E2_2178 and Pf6N2E2_2179 are separated by 76 nucleotides
Pf6N2E2_2177 - Glutamine synthetase (EC 6.3.1.2), putative, at 2,581,513 to 2,582,868
_2177
Pf6N2E2_2178 - FIG00960144: hypothetical protein, at 2,582,852 to 2,583,604
_2178
Pf6N2E2_2179 - Putrescine importer, at 2,583,681 to 2,585,030
_2179
Position (kb)
2582
2583
2584 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2582.092 kb on + strand, within Pf6N2E2_2177 at 2582.151 kb on - strand, within Pf6N2E2_2177 at 2582.353 kb on + strand, within Pf6N2E2_2177 at 2582.398 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.782 kb on + strand at 2582.782 kb on + strand at 2582.782 kb on + strand at 2582.783 kb on - strand at 2582.851 kb on + strand at 2582.851 kb on + strand at 2582.851 kb on + strand at 2582.863 kb on + strand at 2582.863 kb on + strand at 2582.863 kb on + strand at 2582.863 kb on + strand at 2582.864 kb on - strand at 2582.888 kb on + strand at 2582.888 kb on + strand at 2582.889 kb on - strand at 2582.889 kb on - strand at 2583.101 kb on + strand, within Pf6N2E2_2178 at 2583.101 kb on + strand, within Pf6N2E2_2178 at 2583.151 kb on + strand, within Pf6N2E2_2178 at 2583.151 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.174 kb on - strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.392 kb on + strand, within Pf6N2E2_2178 at 2583.392 kb on + strand, within Pf6N2E2_2178 at 2583.455 kb on + strand, within Pf6N2E2_2178 at 2583.455 kb on + strand, within Pf6N2E2_2178 at 2583.584 kb on + strand at 2583.585 kb on - strand at 2583.602 kb on + strand at 2583.602 kb on + strand at 2583.602 kb on + strand at 2583.748 kb on + strand at 2583.748 kb on + strand at 2583.749 kb on - strand at 2583.792 kb on + strand at 2583.798 kb on + strand at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.879 kb on + strand, within Pf6N2E2_2179 at 2583.922 kb on - strand, within Pf6N2E2_2179 at 2583.922 kb on - strand, within Pf6N2E2_2179 at 2584.014 kb on + strand, within Pf6N2E2_2179 at 2584.014 kb on + strand, within Pf6N2E2_2179 at 2584.014 kb on + strand, within Pf6N2E2_2179 at 2584.020 kb on + strand, within Pf6N2E2_2179 at 2584.020 kb on + strand, within Pf6N2E2_2179 at 2584.020 kb on + strand, within Pf6N2E2_2179 at 2584.021 kb on - strand, within Pf6N2E2_2179 at 2584.021 kb on - strand, within Pf6N2E2_2179 at 2584.192 kb on - strand, within Pf6N2E2_2179 at 2584.192 kb on - strand, within Pf6N2E2_2179 at 2584.251 kb on + strand, within Pf6N2E2_2179
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,582,092 + Pf6N2E2_2177 0.43 +1.6 2,582,151 - Pf6N2E2_2177 0.47 +1.6 2,582,353 + Pf6N2E2_2177 0.62 -0.7 2,582,398 + Pf6N2E2_2177 0.65 +0.2 2,582,434 + Pf6N2E2_2177 0.68 -0.3 2,582,434 + Pf6N2E2_2177 0.68 -0.4 2,582,434 + Pf6N2E2_2177 0.68 -0.8 2,582,435 - Pf6N2E2_2177 0.68 +0.5 2,582,435 - Pf6N2E2_2177 0.68 +1.6 2,582,435 - Pf6N2E2_2177 0.68 +1.9 2,582,435 - Pf6N2E2_2177 0.68 +1.2 2,582,782 + +0.5 2,582,782 + -0.8 2,582,782 + -0.2 2,582,783 - +2.4 2,582,851 + +0.2 2,582,851 + +0.5 2,582,851 + -0.9 2,582,863 + -1.8 2,582,863 + -0.9 2,582,863 + +0.1 2,582,863 + -0.2 2,582,864 - +1.2 2,582,888 + +1.2 2,582,888 + +0.8 2,582,889 - +0.8 2,582,889 - +1.7 2,583,101 + Pf6N2E2_2178 0.33 +0.1 2,583,101 + Pf6N2E2_2178 0.33 -0.5 2,583,151 + Pf6N2E2_2178 0.40 -0.8 2,583,151 + Pf6N2E2_2178 0.40 -0.2 2,583,173 + Pf6N2E2_2178 0.43 -0.7 2,583,173 + Pf6N2E2_2178 0.43 -2.7 2,583,173 + Pf6N2E2_2178 0.43 -0.2 2,583,173 + Pf6N2E2_2178 0.43 -1.5 2,583,174 - Pf6N2E2_2178 0.43 -0.2 2,583,338 + Pf6N2E2_2178 0.65 +0.1 2,583,338 + Pf6N2E2_2178 0.65 +0.1 2,583,338 + Pf6N2E2_2178 0.65 -1.5 2,583,338 + Pf6N2E2_2178 0.65 +0.5 2,583,392 + Pf6N2E2_2178 0.72 -0.9 2,583,392 + Pf6N2E2_2178 0.72 -1.0 2,583,455 + Pf6N2E2_2178 0.80 +0.8 2,583,455 + Pf6N2E2_2178 0.80 -0.2 2,583,584 + +0.2 2,583,585 - +1.6 2,583,602 + -0.8 2,583,602 + -1.2 2,583,602 + -1.3 2,583,748 + +1.4 2,583,748 + +0.4 2,583,749 - +0.8 2,583,792 + +0.3 2,583,798 + +1.8 2,583,838 - Pf6N2E2_2179 0.12 +1.4 2,583,838 - Pf6N2E2_2179 0.12 +2.8 2,583,838 - Pf6N2E2_2179 0.12 -0.2 2,583,838 - Pf6N2E2_2179 0.12 +1.8 2,583,879 + Pf6N2E2_2179 0.15 +2.0 2,583,922 - Pf6N2E2_2179 0.18 +0.2 2,583,922 - Pf6N2E2_2179 0.18 +2.6 2,584,014 + Pf6N2E2_2179 0.25 -1.0 2,584,014 + Pf6N2E2_2179 0.25 +1.5 2,584,014 + Pf6N2E2_2179 0.25 +0.8 2,584,020 + Pf6N2E2_2179 0.25 +1.4 2,584,020 + Pf6N2E2_2179 0.25 -0.7 2,584,020 + Pf6N2E2_2179 0.25 +1.0 2,584,021 - Pf6N2E2_2179 0.25 +2.4 2,584,021 - Pf6N2E2_2179 0.25 +0.6 2,584,192 - Pf6N2E2_2179 0.38 -0.1 2,584,192 - Pf6N2E2_2179 0.38 +1.2 2,584,251 + Pf6N2E2_2179 0.42 +0.4
Or see this region's nucleotide sequence