Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_203 and Pf6N2E2_204 are separated by 2 nucleotides Pf6N2E2_204 and Pf6N2E2_205 overlap by 4 nucleotides Pf6N2E2_205 and Pf6N2E2_206 are separated by 32 nucleotides
Pf6N2E2_203 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 254,640 to 255,920
_203
Pf6N2E2_204 - hypothetical protein, at 255,923 to 256,330
_204
Pf6N2E2_205 - hypothetical protein, at 256,327 to 257,073
_205
Pf6N2E2_206 - Glycosyl transferase, group 2 family protein, at 257,106 to 258,128
_206
Position (kb) 255
256
257 Strain fitness (log2 ratio) -2
-1
0
1 at 254.960 kb on + strand, within Pf6N2E2_203 at 255.029 kb on - strand, within Pf6N2E2_203 at 255.232 kb on - strand, within Pf6N2E2_203 at 255.384 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.417 kb on - strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.805 kb on + strand at 255.805 kb on + strand at 255.805 kb on + strand at 255.805 kb on + strand at 255.806 kb on - strand at 255.806 kb on - strand at 255.806 kb on - strand at 255.890 kb on - strand at 255.912 kb on + strand at 256.062 kb on + strand, within Pf6N2E2_204 at 256.062 kb on + strand, within Pf6N2E2_204 at 256.062 kb on + strand, within Pf6N2E2_204 at 256.321 kb on + strand at 256.478 kb on + strand, within Pf6N2E2_205 at 256.478 kb on + strand, within Pf6N2E2_205 at 256.479 kb on - strand, within Pf6N2E2_205 at 256.562 kb on + strand, within Pf6N2E2_205 at 256.562 kb on + strand, within Pf6N2E2_205 at 256.563 kb on - strand, within Pf6N2E2_205 at 256.563 kb on - strand, within Pf6N2E2_205 at 256.563 kb on - strand, within Pf6N2E2_205 at 256.659 kb on + strand, within Pf6N2E2_205 at 256.661 kb on + strand, within Pf6N2E2_205 at 256.661 kb on + strand, within Pf6N2E2_205 at 256.882 kb on + strand, within Pf6N2E2_205 at 256.883 kb on - strand, within Pf6N2E2_205 at 256.892 kb on + strand, within Pf6N2E2_205 at 256.956 kb on - strand, within Pf6N2E2_205 at 257.148 kb on - strand at 257.305 kb on + strand, within Pf6N2E2_206 at 257.305 kb on + strand, within Pf6N2E2_206 at 257.306 kb on - strand, within Pf6N2E2_206 at 257.306 kb on - strand, within Pf6N2E2_206 at 257.306 kb on - strand, within Pf6N2E2_206
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 254,960 + Pf6N2E2_203 0.25 -2.0 255,029 - Pf6N2E2_203 0.30 +1.4 255,232 - Pf6N2E2_203 0.46 -0.8 255,384 + Pf6N2E2_203 0.58 +1.7 255,416 + Pf6N2E2_203 0.61 -0.7 255,416 + Pf6N2E2_203 0.61 -0.8 255,416 + Pf6N2E2_203 0.61 +1.1 255,416 + Pf6N2E2_203 0.61 +0.4 255,416 + Pf6N2E2_203 0.61 +0.2 255,416 + Pf6N2E2_203 0.61 +1.3 255,417 - Pf6N2E2_203 0.61 +0.8 255,514 + Pf6N2E2_203 0.68 +0.5 255,514 + Pf6N2E2_203 0.68 +0.4 255,514 + Pf6N2E2_203 0.68 +0.4 255,515 - Pf6N2E2_203 0.68 +0.1 255,515 - Pf6N2E2_203 0.68 -2.2 255,515 - Pf6N2E2_203 0.68 -0.8 255,803 + +0.8 255,803 + -0.2 255,803 + -0.3 255,803 + -0.8 255,803 + +0.1 255,803 + -0.1 255,803 + +1.0 255,805 + -0.2 255,805 + -0.4 255,805 + -0.1 255,805 + -0.4 255,806 - +1.4 255,806 - +0.8 255,806 - -1.8 255,890 - -0.2 255,912 + +0.1 256,062 + Pf6N2E2_204 0.34 -0.8 256,062 + Pf6N2E2_204 0.34 -0.5 256,062 + Pf6N2E2_204 0.34 -0.2 256,321 + -0.3 256,478 + Pf6N2E2_205 0.20 -0.5 256,478 + Pf6N2E2_205 0.20 -0.4 256,479 - Pf6N2E2_205 0.20 -0.6 256,562 + Pf6N2E2_205 0.31 +0.8 256,562 + Pf6N2E2_205 0.31 +0.5 256,563 - Pf6N2E2_205 0.32 -0.1 256,563 - Pf6N2E2_205 0.32 +1.3 256,563 - Pf6N2E2_205 0.32 -1.4 256,659 + Pf6N2E2_205 0.44 +0.8 256,661 + Pf6N2E2_205 0.45 -1.2 256,661 + Pf6N2E2_205 0.45 -1.1 256,882 + Pf6N2E2_205 0.74 -0.2 256,883 - Pf6N2E2_205 0.74 +0.8 256,892 + Pf6N2E2_205 0.76 +0.4 256,956 - Pf6N2E2_205 0.84 -0.4 257,148 - +0.6 257,305 + Pf6N2E2_206 0.19 -0.2 257,305 + Pf6N2E2_206 0.19 +0.6 257,306 - Pf6N2E2_206 0.20 -2.0 257,306 - Pf6N2E2_206 0.20 -1.1 257,306 - Pf6N2E2_206 0.20 +0.1
Or see this region's nucleotide sequence