Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_192

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_191 and Pf6N2E2_192 are separated by 217 nucleotidesPf6N2E2_192 and Pf6N2E2_193 are separated by 377 nucleotidesPf6N2E2_193 and Pf6N2E2_194 overlap by 36 nucleotides Pf6N2E2_191 - ClpB protein, at 243,415 to 244,458 _191 Pf6N2E2_192 - hypothetical protein, at 244,676 to 246,115 _192 Pf6N2E2_193 - hypothetical protein, at 246,493 to 246,870 _193 Pf6N2E2_194 - Alcohol dehydrogenase (EC 1.1.1.1), at 246,835 to 247,569 _194 Position (kb) 244 245 246 247Strain fitness (log2 ratio) -2 -1 0 1 2 3at 243.686 kb on + strand, within Pf6N2E2_191at 243.687 kb on - strand, within Pf6N2E2_191at 243.687 kb on - strand, within Pf6N2E2_191at 243.707 kb on + strand, within Pf6N2E2_191at 243.707 kb on + strand, within Pf6N2E2_191at 243.707 kb on + strand, within Pf6N2E2_191at 243.708 kb on - strand, within Pf6N2E2_191at 243.799 kb on + strand, within Pf6N2E2_191at 243.799 kb on + strand, within Pf6N2E2_191at 243.810 kb on + strand, within Pf6N2E2_191at 243.856 kb on + strand, within Pf6N2E2_191at 243.856 kb on + strand, within Pf6N2E2_191at 243.857 kb on - strand, within Pf6N2E2_191at 243.857 kb on - strand, within Pf6N2E2_191at 243.857 kb on - strand, within Pf6N2E2_191at 244.040 kb on - strand, within Pf6N2E2_191at 244.104 kb on + strand, within Pf6N2E2_191at 244.104 kb on + strand, within Pf6N2E2_191at 244.105 kb on - strand, within Pf6N2E2_191at 244.457 kb on - strandat 244.590 kb on + strandat 244.591 kb on - strandat 244.591 kb on - strandat 244.591 kb on - strandat 244.591 kb on - strandat 244.596 kb on + strandat 244.596 kb on + strandat 244.596 kb on + strandat 244.643 kb on + strandat 244.648 kb on + strandat 244.648 kb on + strandat 244.648 kb on + strandat 244.648 kb on + strandat 244.649 kb on - strandat 244.649 kb on - strandat 244.797 kb on + strandat 244.878 kb on + strand, within Pf6N2E2_192at 244.878 kb on + strand, within Pf6N2E2_192at 244.878 kb on + strand, within Pf6N2E2_192at 244.879 kb on - strand, within Pf6N2E2_192at 244.879 kb on - strand, within Pf6N2E2_192at 244.879 kb on - strand, within Pf6N2E2_192at 244.879 kb on - strand, within Pf6N2E2_192at 244.879 kb on - strand, within Pf6N2E2_192at 244.947 kb on + strand, within Pf6N2E2_192at 244.947 kb on + strand, within Pf6N2E2_192at 244.947 kb on + strand, within Pf6N2E2_192at 244.988 kb on + strand, within Pf6N2E2_192at 244.989 kb on - strand, within Pf6N2E2_192at 244.989 kb on - strand, within Pf6N2E2_192at 244.992 kb on + strand, within Pf6N2E2_192at 244.992 kb on + strand, within Pf6N2E2_192at 244.993 kb on - strand, within Pf6N2E2_192at 244.998 kb on + strand, within Pf6N2E2_192at 244.998 kb on + strand, within Pf6N2E2_192at 244.998 kb on + strand, within Pf6N2E2_192at 244.998 kb on + strand, within Pf6N2E2_192at 244.998 kb on + strand, within Pf6N2E2_192at 244.999 kb on - strand, within Pf6N2E2_192at 244.999 kb on - strand, within Pf6N2E2_192at 244.999 kb on - strand, within Pf6N2E2_192at 244.999 kb on - strand, within Pf6N2E2_192at 244.999 kb on - strand, within Pf6N2E2_192at 245.024 kb on + strand, within Pf6N2E2_192at 245.061 kb on + strand, within Pf6N2E2_192at 245.061 kb on + strand, within Pf6N2E2_192at 245.149 kb on + strand, within Pf6N2E2_192at 245.150 kb on - strand, within Pf6N2E2_192at 245.151 kb on + strand, within Pf6N2E2_192at 245.151 kb on + strand, within Pf6N2E2_192at 245.152 kb on - strand, within Pf6N2E2_192at 245.301 kb on + strand, within Pf6N2E2_192at 245.397 kb on + strand, within Pf6N2E2_192at 245.397 kb on + strand, within Pf6N2E2_192at 245.398 kb on - strand, within Pf6N2E2_192at 245.591 kb on + strand, within Pf6N2E2_192at 245.591 kb on + strand, within Pf6N2E2_192at 245.591 kb on + strand, within Pf6N2E2_192at 245.592 kb on - strand, within Pf6N2E2_192at 245.592 kb on - strand, within Pf6N2E2_192at 245.780 kb on - strand, within Pf6N2E2_192at 245.833 kb on + strand, within Pf6N2E2_192at 245.834 kb on - strand, within Pf6N2E2_192at 245.861 kb on + strand, within Pf6N2E2_192at 246.091 kb on - strandat 246.091 kb on - strandat 246.173 kb on + strandat 246.173 kb on + strandat 246.174 kb on - strandat 246.174 kb on - strandat 246.249 kb on - strandat 246.253 kb on + strandat 246.254 kb on - strandat 246.254 kb on - strandat 246.254 kb on - strandat 246.254 kb on - strandat 246.286 kb on + strandat 246.286 kb on + strandat 246.304 kb on + strandat 246.304 kb on + strandat 246.304 kb on + strandat 246.305 kb on - strandat 246.305 kb on - strandat 246.305 kb on - strandat 246.305 kb on - strandat 246.305 kb on - strandat 246.353 kb on - strandat 246.462 kb on + strandat 246.465 kb on + strandat 246.465 kb on + strandat 246.465 kb on + strandat 246.498 kb on + strandat 246.498 kb on + strandat 246.500 kb on + strandat 246.511 kb on + strandat 246.511 kb on + strandat 246.512 kb on - strandat 246.517 kb on - strandat 246.539 kb on + strand, within Pf6N2E2_193at 246.539 kb on + strand, within Pf6N2E2_193at 246.539 kb on + strand, within Pf6N2E2_193at 246.544 kb on + strand, within Pf6N2E2_193at 246.544 kb on + strand, within Pf6N2E2_193at 246.772 kb on - strand, within Pf6N2E2_193at 246.889 kb on + strandat 246.889 kb on + strandat 246.889 kb on + strandat 246.890 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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243,686 + Pf6N2E2_191 0.26 +0.1
243,687 - Pf6N2E2_191 0.26 -1.2
243,687 - Pf6N2E2_191 0.26 -0.9
243,707 + Pf6N2E2_191 0.28 +0.1
243,707 + Pf6N2E2_191 0.28 +0.8
243,707 + Pf6N2E2_191 0.28 +1.4
243,708 - Pf6N2E2_191 0.28 +0.5
243,799 + Pf6N2E2_191 0.37 +1.3
243,799 + Pf6N2E2_191 0.37 -0.5
243,810 + Pf6N2E2_191 0.38 +0.4
243,856 + Pf6N2E2_191 0.42 +0.8
243,856 + Pf6N2E2_191 0.42 -1.2
243,857 - Pf6N2E2_191 0.42 +0.7
243,857 - Pf6N2E2_191 0.42 -0.2
243,857 - Pf6N2E2_191 0.42 +0.8
244,040 - Pf6N2E2_191 0.60 -0.2
244,104 + Pf6N2E2_191 0.66 +0.8
244,104 + Pf6N2E2_191 0.66 -0.4
244,105 - Pf6N2E2_191 0.66 +0.5
244,457 - +0.2
244,590 + -0.4
244,591 - -0.2
244,591 - -0.1
244,591 - +0.2
244,591 - -1.1
244,596 + -0.6
244,596 + -1.4
244,596 + +0.5
244,643 + +0.8
244,648 + +0.3
244,648 + -1.8
244,648 + -0.2
244,648 + +0.2
244,649 - -0.2
244,649 - -0.6
244,797 + -2.5
244,878 + Pf6N2E2_192 0.14 -0.2
244,878 + Pf6N2E2_192 0.14 -0.2
244,878 + Pf6N2E2_192 0.14 -0.4
244,879 - Pf6N2E2_192 0.14 -0.2
244,879 - Pf6N2E2_192 0.14 -0.0
244,879 - Pf6N2E2_192 0.14 +0.6
244,879 - Pf6N2E2_192 0.14 -0.9
244,879 - Pf6N2E2_192 0.14 -0.8
244,947 + Pf6N2E2_192 0.19 -0.2
244,947 + Pf6N2E2_192 0.19 +2.4
244,947 + Pf6N2E2_192 0.19 +0.5
244,988 + Pf6N2E2_192 0.22 -0.9
244,989 - Pf6N2E2_192 0.22 -0.7
244,989 - Pf6N2E2_192 0.22 +0.8
244,992 + Pf6N2E2_192 0.22 -1.2
244,992 + Pf6N2E2_192 0.22 +0.5
244,993 - Pf6N2E2_192 0.22 -0.8
244,998 + Pf6N2E2_192 0.22 +0.5
244,998 + Pf6N2E2_192 0.22 -1.0
244,998 + Pf6N2E2_192 0.22 +0.5
244,998 + Pf6N2E2_192 0.22 -0.8
244,998 + Pf6N2E2_192 0.22 +0.8
244,999 - Pf6N2E2_192 0.22 +0.1
244,999 - Pf6N2E2_192 0.22 +0.4
244,999 - Pf6N2E2_192 0.22 -0.0
244,999 - Pf6N2E2_192 0.22 -1.0
244,999 - Pf6N2E2_192 0.22 +0.8
245,024 + Pf6N2E2_192 0.24 -0.5
245,061 + Pf6N2E2_192 0.27 +0.4
245,061 + Pf6N2E2_192 0.27 -0.5
245,149 + Pf6N2E2_192 0.33 -0.5
245,150 - Pf6N2E2_192 0.33 -0.2
245,151 + Pf6N2E2_192 0.33 -0.8
245,151 + Pf6N2E2_192 0.33 -0.1
245,152 - Pf6N2E2_192 0.33 +1.4
245,301 + Pf6N2E2_192 0.43 -1.8
245,397 + Pf6N2E2_192 0.50 +0.1
245,397 + Pf6N2E2_192 0.50 -1.0
245,398 - Pf6N2E2_192 0.50 +0.3
245,591 + Pf6N2E2_192 0.64 +0.1
245,591 + Pf6N2E2_192 0.64 -0.2
245,591 + Pf6N2E2_192 0.64 -0.8
245,592 - Pf6N2E2_192 0.64 +1.3
245,592 - Pf6N2E2_192 0.64 -0.8
245,780 - Pf6N2E2_192 0.77 -1.2
245,833 + Pf6N2E2_192 0.80 -0.5
245,834 - Pf6N2E2_192 0.80 -1.1
245,861 + Pf6N2E2_192 0.82 -0.7
246,091 - -1.8
246,091 - -0.2
246,173 + -1.3
246,173 + +0.1
246,174 - +0.1
246,174 - -0.3
246,249 - +0.1
246,253 + -0.9
246,254 - +0.8
246,254 - +0.5
246,254 - +0.5
246,254 - +1.3
246,286 + +0.3
246,286 + -0.8
246,304 + +0.2
246,304 + +2.8
246,304 + +0.3
246,305 - -0.0
246,305 - -1.2
246,305 - -0.0
246,305 - -0.5
246,305 - -0.0
246,353 - +0.8
246,462 + +1.3
246,465 + +2.4
246,465 + -0.4
246,465 + +0.3
246,498 + +1.1
246,498 + +0.3
246,500 + -0.2
246,511 + +0.4
246,511 + +2.1
246,512 - -0.9
246,517 - +0.8
246,539 + Pf6N2E2_193 0.12 -0.1
246,539 + Pf6N2E2_193 0.12 +0.8
246,539 + Pf6N2E2_193 0.12 +0.5
246,544 + Pf6N2E2_193 0.13 -0.8
246,544 + Pf6N2E2_193 0.13 -0.1
246,772 - Pf6N2E2_193 0.74 -1.2
246,889 + -0.1
246,889 + +0.6
246,889 + -0.2
246,890 - +0.8

Or see this region's nucleotide sequence