Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1495

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1493 and Pf6N2E2_1494 overlap by 14 nucleotidesPf6N2E2_1494 and Pf6N2E2_1495 overlap by 11 nucleotidesPf6N2E2_1495 and Pf6N2E2_1496 overlap by 4 nucleotides Pf6N2E2_1493 - ABC-type polar amino acid transport system, ATPase component, at 1,747,150 to 1,747,986 _1493 Pf6N2E2_1494 - Glutamate transport membrane-spanning protein, at 1,747,973 to 1,748,761 _1494 Pf6N2E2_1495 - Glutamate transport membrane-spanning protein, at 1,748,751 to 1,749,485 _1495 Pf6N2E2_1496 - Aspartate aminotransferase (EC 2.6.1.1), at 1,749,482 to 1,750,693 _1496 Position (kb) 1748 1749 1750Strain fitness (log2 ratio) -2 -1 0 1 2at 1747.977 kb on + strandat 1748.008 kb on + strandat 1748.009 kb on - strandat 1748.031 kb on + strandat 1748.062 kb on - strand, within Pf6N2E2_1494at 1748.062 kb on - strand, within Pf6N2E2_1494at 1748.179 kb on - strand, within Pf6N2E2_1494at 1748.179 kb on - strand, within Pf6N2E2_1494at 1748.427 kb on + strand, within Pf6N2E2_1494at 1748.427 kb on + strand, within Pf6N2E2_1494at 1748.427 kb on + strand, within Pf6N2E2_1494at 1748.427 kb on + strand, within Pf6N2E2_1494at 1748.427 kb on + strand, within Pf6N2E2_1494at 1748.427 kb on + strand, within Pf6N2E2_1494at 1748.428 kb on - strand, within Pf6N2E2_1494at 1748.428 kb on - strand, within Pf6N2E2_1494at 1748.428 kb on - strand, within Pf6N2E2_1494at 1748.439 kb on + strand, within Pf6N2E2_1494at 1748.665 kb on - strand, within Pf6N2E2_1494at 1748.665 kb on - strand, within Pf6N2E2_1494at 1748.716 kb on + strandat 1748.899 kb on + strand, within Pf6N2E2_1495at 1748.899 kb on + strand, within Pf6N2E2_1495at 1748.900 kb on - strand, within Pf6N2E2_1495at 1748.900 kb on - strand, within Pf6N2E2_1495at 1748.900 kb on - strand, within Pf6N2E2_1495at 1749.223 kb on + strand, within Pf6N2E2_1495at 1749.224 kb on - strand, within Pf6N2E2_1495at 1749.224 kb on - strand, within Pf6N2E2_1495at 1749.224 kb on - strand, within Pf6N2E2_1495at 1749.224 kb on - strand, within Pf6N2E2_1495at 1749.409 kb on + strand, within Pf6N2E2_1495at 1749.431 kb on - strandat 1749.592 kb on - strandat 1749.798 kb on + strand, within Pf6N2E2_1496at 1749.969 kb on + strand, within Pf6N2E2_1496at 1749.969 kb on + strand, within Pf6N2E2_1496at 1749.970 kb on - strand, within Pf6N2E2_1496at 1750.063 kb on + strand, within Pf6N2E2_1496at 1750.071 kb on + strand, within Pf6N2E2_1496

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,747,977 + -0.5
1,748,008 + +0.1
1,748,009 - -2.5
1,748,031 + -0.4
1,748,062 - Pf6N2E2_1494 0.11 +0.5
1,748,062 - Pf6N2E2_1494 0.11 -1.4
1,748,179 - Pf6N2E2_1494 0.26 -0.9
1,748,179 - Pf6N2E2_1494 0.26 -0.2
1,748,427 + Pf6N2E2_1494 0.58 +0.5
1,748,427 + Pf6N2E2_1494 0.58 +0.3
1,748,427 + Pf6N2E2_1494 0.58 +0.5
1,748,427 + Pf6N2E2_1494 0.58 -0.0
1,748,427 + Pf6N2E2_1494 0.58 +0.5
1,748,427 + Pf6N2E2_1494 0.58 -0.8
1,748,428 - Pf6N2E2_1494 0.58 -1.5
1,748,428 - Pf6N2E2_1494 0.58 +0.5
1,748,428 - Pf6N2E2_1494 0.58 +0.9
1,748,439 + Pf6N2E2_1494 0.59 +2.1
1,748,665 - Pf6N2E2_1494 0.88 -0.6
1,748,665 - Pf6N2E2_1494 0.88 +0.2
1,748,716 + +0.7
1,748,899 + Pf6N2E2_1495 0.20 -0.5
1,748,899 + Pf6N2E2_1495 0.20 +0.1
1,748,900 - Pf6N2E2_1495 0.20 -0.8
1,748,900 - Pf6N2E2_1495 0.20 -0.0
1,748,900 - Pf6N2E2_1495 0.20 -1.0
1,749,223 + Pf6N2E2_1495 0.64 -0.5
1,749,224 - Pf6N2E2_1495 0.64 -0.7
1,749,224 - Pf6N2E2_1495 0.64 -0.9
1,749,224 - Pf6N2E2_1495 0.64 -1.2
1,749,224 - Pf6N2E2_1495 0.64 -1.0
1,749,409 + Pf6N2E2_1495 0.90 -1.0
1,749,431 - -0.3
1,749,592 - -0.7
1,749,798 + Pf6N2E2_1496 0.26 +0.5
1,749,969 + Pf6N2E2_1496 0.40 -0.5
1,749,969 + Pf6N2E2_1496 0.40 -1.3
1,749,970 - Pf6N2E2_1496 0.40 -2.5
1,750,063 + Pf6N2E2_1496 0.48 +1.0
1,750,071 + Pf6N2E2_1496 0.49 +0.1

Or see this region's nucleotide sequence