Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1491 and Pf6N2E2_1492 are separated by 45 nucleotides Pf6N2E2_1492 and Pf6N2E2_1493 are separated by 27 nucleotides Pf6N2E2_1493 and Pf6N2E2_1494 overlap by 14 nucleotides
Pf6N2E2_1491 - Cystathionine beta-lyase (EC 4.4.1.8), at 1,743,712 to 1,746,477
_1491
Pf6N2E2_1492 - No significant database matches, at 1,746,523 to 1,747,122
_1492
Pf6N2E2_1493 - ABC-type polar amino acid transport system, ATPase component, at 1,747,150 to 1,747,986
_1493
Pf6N2E2_1494 - Glutamate transport membrane-spanning protein, at 1,747,973 to 1,748,761
_1494
Position (kb)
1746
1747
1748 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1745.534 kb on + strand, within Pf6N2E2_1491 at 1745.534 kb on + strand, within Pf6N2E2_1491 at 1745.534 kb on + strand, within Pf6N2E2_1491 at 1745.535 kb on - strand, within Pf6N2E2_1491 at 1745.535 kb on - strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.841 kb on - strand, within Pf6N2E2_1491 at 1745.841 kb on - strand, within Pf6N2E2_1491 at 1745.841 kb on - strand, within Pf6N2E2_1491 at 1746.118 kb on + strand, within Pf6N2E2_1491 at 1746.119 kb on - strand, within Pf6N2E2_1491 at 1746.119 kb on - strand, within Pf6N2E2_1491 at 1746.346 kb on - strand at 1746.463 kb on + strand at 1746.464 kb on - strand at 1746.534 kb on - strand at 1746.569 kb on + strand at 1746.570 kb on - strand at 1746.636 kb on - strand, within Pf6N2E2_1492 at 1746.715 kb on - strand, within Pf6N2E2_1492 at 1746.715 kb on - strand, within Pf6N2E2_1492 at 1746.758 kb on + strand, within Pf6N2E2_1492 at 1746.792 kb on - strand, within Pf6N2E2_1492 at 1747.117 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.243 kb on + strand, within Pf6N2E2_1493 at 1747.332 kb on - strand, within Pf6N2E2_1493 at 1747.382 kb on - strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.695 kb on - strand, within Pf6N2E2_1493 at 1747.695 kb on - strand, within Pf6N2E2_1493 at 1747.977 kb on + strand at 1748.008 kb on + strand at 1748.009 kb on - strand at 1748.031 kb on + strand at 1748.062 kb on - strand, within Pf6N2E2_1494 at 1748.062 kb on - strand, within Pf6N2E2_1494
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,745,534 + Pf6N2E2_1491 0.66 -0.5 1,745,534 + Pf6N2E2_1491 0.66 +0.5 1,745,534 + Pf6N2E2_1491 0.66 -0.2 1,745,535 - Pf6N2E2_1491 0.66 +1.0 1,745,535 - Pf6N2E2_1491 0.66 -0.1 1,745,840 + Pf6N2E2_1491 0.77 +0.5 1,745,840 + Pf6N2E2_1491 0.77 +0.9 1,745,840 + Pf6N2E2_1491 0.77 -0.2 1,745,840 + Pf6N2E2_1491 0.77 +1.3 1,745,841 - Pf6N2E2_1491 0.77 +0.5 1,745,841 - Pf6N2E2_1491 0.77 +0.4 1,745,841 - Pf6N2E2_1491 0.77 -0.5 1,746,118 + Pf6N2E2_1491 0.87 -0.7 1,746,119 - Pf6N2E2_1491 0.87 -0.2 1,746,119 - Pf6N2E2_1491 0.87 +0.2 1,746,346 - +0.5 1,746,463 + -3.6 1,746,464 - -0.9 1,746,534 - +1.0 1,746,569 + -0.1 1,746,570 - -0.6 1,746,636 - Pf6N2E2_1492 0.19 -0.2 1,746,715 - Pf6N2E2_1492 0.32 +0.5 1,746,715 - Pf6N2E2_1492 0.32 -0.8 1,746,758 + Pf6N2E2_1492 0.39 -1.0 1,746,792 - Pf6N2E2_1492 0.45 -0.9 1,747,117 - -1.8 1,747,191 - +0.5 1,747,191 - -2.7 1,747,191 - -0.8 1,747,243 + Pf6N2E2_1493 0.11 -0.2 1,747,332 - Pf6N2E2_1493 0.22 +1.1 1,747,382 - Pf6N2E2_1493 0.28 +1.1 1,747,694 + Pf6N2E2_1493 0.65 +1.0 1,747,694 + Pf6N2E2_1493 0.65 +0.9 1,747,694 + Pf6N2E2_1493 0.65 +1.5 1,747,694 + Pf6N2E2_1493 0.65 +1.4 1,747,695 - Pf6N2E2_1493 0.65 -0.2 1,747,695 - Pf6N2E2_1493 0.65 -0.3 1,747,977 + -0.5 1,748,008 + +0.1 1,748,009 - -2.5 1,748,031 + -0.4 1,748,062 - Pf6N2E2_1494 0.11 +0.5 1,748,062 - Pf6N2E2_1494 0.11 -1.4
Or see this region's nucleotide sequence