Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1435 and Pf6N2E2_1436 are separated by 40 nucleotides Pf6N2E2_1436 and Pf6N2E2_1437 are separated by 377 nucleotides Pf6N2E2_1437 and Pf6N2E2_1438 are separated by 527 nucleotides
Pf6N2E2_1435 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase, at 1,684,697 to 1,685,794
_1435
Pf6N2E2_1436 - FMN reductase (EC 1.5.1.29), at 1,685,835 to 1,686,398
_1436
Pf6N2E2_1437 - Acyl-CoA dehydrogenase family protein, at 1,686,776 to 1,688,020
_1437
Pf6N2E2_1438 - Methyltransferase (EC 2.1.1.-), at 1,688,548 to 1,689,132
_1438
Position (kb)
1686
1687
1688
1689 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.801 kb on + strand at 1685.802 kb on - strand at 1685.802 kb on - strand at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1686.079 kb on - strand, within Pf6N2E2_1436 at 1686.137 kb on - strand, within Pf6N2E2_1436 at 1686.137 kb on - strand, within Pf6N2E2_1436 at 1686.149 kb on - strand, within Pf6N2E2_1436 at 1686.447 kb on - strand at 1686.483 kb on - strand at 1687.254 kb on - strand, within Pf6N2E2_1437 at 1687.256 kb on + strand, within Pf6N2E2_1437 at 1687.256 kb on + strand, within Pf6N2E2_1437 at 1687.257 kb on - strand, within Pf6N2E2_1437 at 1687.270 kb on + strand, within Pf6N2E2_1437 at 1687.295 kb on + strand, within Pf6N2E2_1437 at 1687.295 kb on + strand, within Pf6N2E2_1437 at 1687.344 kb on + strand, within Pf6N2E2_1437 at 1687.662 kb on - strand, within Pf6N2E2_1437 at 1687.712 kb on + strand, within Pf6N2E2_1437 at 1687.931 kb on + strand at 1687.931 kb on + strand at 1687.931 kb on + strand at 1687.932 kb on - strand at 1687.982 kb on + strand at 1687.983 kb on - strand at 1688.022 kb on + strand at 1688.022 kb on + strand at 1688.066 kb on + strand at 1688.067 kb on - strand at 1688.215 kb on + strand at 1688.216 kb on - strand at 1688.216 kb on - strand at 1688.282 kb on - strand at 1688.282 kb on - strand at 1688.298 kb on - strand at 1688.323 kb on - strand at 1688.323 kb on - strand at 1688.331 kb on + strand at 1688.332 kb on - strand at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.396 kb on - strand at 1688.416 kb on + strand at 1688.416 kb on + strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.450 kb on + strand at 1688.507 kb on + strand at 1688.508 kb on - strand at 1688.744 kb on + strand, within Pf6N2E2_1438 at 1688.745 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.930 kb on - strand, within Pf6N2E2_1438 at 1688.988 kb on + strand, within Pf6N2E2_1438 at 1688.989 kb on - strand, within Pf6N2E2_1438
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,685,795 + +0.4 1,685,795 + -2.8 1,685,795 + +0.5 1,685,795 + +1.1 1,685,795 + -0.1 1,685,795 + -0.2 1,685,795 + +0.1 1,685,795 + +0.8 1,685,795 + +0.5 1,685,795 + -0.5 1,685,795 + -0.7 1,685,795 + +0.1 1,685,795 + -0.1 1,685,796 - +0.8 1,685,796 - +0.3 1,685,796 - -0.2 1,685,796 - -1.8 1,685,796 - +1.8 1,685,796 - -0.6 1,685,796 - +0.3 1,685,801 + +0.5 1,685,802 - -0.5 1,685,802 - +2.5 1,685,965 + Pf6N2E2_1436 0.23 -0.2 1,685,965 + Pf6N2E2_1436 0.23 -1.5 1,685,965 + Pf6N2E2_1436 0.23 +0.8 1,685,965 + Pf6N2E2_1436 0.23 -1.8 1,685,965 + Pf6N2E2_1436 0.23 +1.7 1,686,079 - Pf6N2E2_1436 0.43 -0.5 1,686,137 - Pf6N2E2_1436 0.54 -2.6 1,686,137 - Pf6N2E2_1436 0.54 +1.1 1,686,149 - Pf6N2E2_1436 0.56 +0.9 1,686,447 - -0.7 1,686,483 - +0.4 1,687,254 - Pf6N2E2_1437 0.38 +0.5 1,687,256 + Pf6N2E2_1437 0.39 -1.6 1,687,256 + Pf6N2E2_1437 0.39 +1.1 1,687,257 - Pf6N2E2_1437 0.39 -2.1 1,687,270 + Pf6N2E2_1437 0.40 -0.5 1,687,295 + Pf6N2E2_1437 0.42 -1.1 1,687,295 + Pf6N2E2_1437 0.42 -1.3 1,687,344 + Pf6N2E2_1437 0.46 +1.2 1,687,662 - Pf6N2E2_1437 0.71 -0.3 1,687,712 + Pf6N2E2_1437 0.75 -1.1 1,687,931 + -0.5 1,687,931 + -0.2 1,687,931 + -2.3 1,687,932 - +0.9 1,687,982 + -1.1 1,687,983 - -0.8 1,688,022 + +0.9 1,688,022 + +0.1 1,688,066 + +0.5 1,688,067 - +1.5 1,688,215 + +1.1 1,688,216 - +2.9 1,688,216 - +0.5 1,688,282 - -0.4 1,688,282 - -0.2 1,688,298 - -1.1 1,688,323 - +0.2 1,688,323 - +0.0 1,688,331 + +0.5 1,688,332 - -0.7 1,688,391 - -0.4 1,688,391 - -0.2 1,688,391 - -0.5 1,688,396 - +0.5 1,688,416 + +0.1 1,688,416 + -1.5 1,688,417 - +0.9 1,688,417 - +1.5 1,688,417 - +1.1 1,688,450 + +0.5 1,688,507 + +1.5 1,688,508 - -0.9 1,688,744 + Pf6N2E2_1438 0.34 +0.9 1,688,745 - Pf6N2E2_1438 0.34 +0.1 1,688,821 - Pf6N2E2_1438 0.47 +0.5 1,688,821 - Pf6N2E2_1438 0.47 +0.9 1,688,930 - Pf6N2E2_1438 0.65 -0.1 1,688,988 + Pf6N2E2_1438 0.75 -0.1 1,688,989 - Pf6N2E2_1438 0.75 +0.0
Or see this region's nucleotide sequence