Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1312

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1310 and Pf6N2E2_1311 are separated by 91 nucleotidesPf6N2E2_1311 and Pf6N2E2_1312 are separated by 52 nucleotidesPf6N2E2_1312 and Pf6N2E2_1313 are separated by 12 nucleotidesPf6N2E2_1313 and Pf6N2E2_1314 are separated by 14 nucleotides Pf6N2E2_1310 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 1,544,395 to 1,545,426 _1310 Pf6N2E2_1311 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 1,545,518 to 1,545,910 _1311 Pf6N2E2_1312 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 1,545,963 to 1,547,267 _1312 Pf6N2E2_1313 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 1,547,280 to 1,548,083 _1313 Pf6N2E2_1314 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,548,098 to 1,548,895 _1314 Position (kb) 1545 1546 1547 1548Strain fitness (log2 ratio) -1 0 1 2at 1544.965 kb on + strand, within Pf6N2E2_1310at 1544.966 kb on - strand, within Pf6N2E2_1310at 1544.966 kb on - strand, within Pf6N2E2_1310at 1545.347 kb on - strandat 1545.450 kb on - strandat 1545.450 kb on - strandat 1545.480 kb on + strandat 1545.703 kb on - strand, within Pf6N2E2_1311at 1545.703 kb on - strand, within Pf6N2E2_1311at 1546.063 kb on - strandat 1546.311 kb on + strand, within Pf6N2E2_1312at 1546.731 kb on + strand, within Pf6N2E2_1312at 1546.732 kb on - strand, within Pf6N2E2_1312at 1546.732 kb on - strand, within Pf6N2E2_1312at 1546.732 kb on - strand, within Pf6N2E2_1312at 1546.757 kb on + strand, within Pf6N2E2_1312at 1547.140 kb on - strandat 1547.190 kb on + strandat 1547.191 kb on - strandat 1547.191 kb on - strandat 1547.191 kb on - strandat 1547.191 kb on - strandat 1547.397 kb on + strand, within Pf6N2E2_1313at 1547.398 kb on - strand, within Pf6N2E2_1313at 1547.856 kb on + strand, within Pf6N2E2_1313at 1547.856 kb on + strand, within Pf6N2E2_1313at 1547.953 kb on - strand, within Pf6N2E2_1313at 1548.182 kb on + strand, within Pf6N2E2_1314

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,544,965 + Pf6N2E2_1310 0.55 +1.1
1,544,966 - Pf6N2E2_1310 0.55 +0.2
1,544,966 - Pf6N2E2_1310 0.55 -1.2
1,545,347 - -0.5
1,545,450 - +1.5
1,545,450 - -0.7
1,545,480 + +0.9
1,545,703 - Pf6N2E2_1311 0.47 -1.5
1,545,703 - Pf6N2E2_1311 0.47 -1.3
1,546,063 - -0.9
1,546,311 + Pf6N2E2_1312 0.27 +0.5
1,546,731 + Pf6N2E2_1312 0.59 -0.8
1,546,732 - Pf6N2E2_1312 0.59 -0.5
1,546,732 - Pf6N2E2_1312 0.59 -1.5
1,546,732 - Pf6N2E2_1312 0.59 -1.1
1,546,757 + Pf6N2E2_1312 0.61 +0.5
1,547,140 - -1.2
1,547,190 + -0.1
1,547,191 - -0.9
1,547,191 - -0.5
1,547,191 - +0.5
1,547,191 - +0.5
1,547,397 + Pf6N2E2_1313 0.15 -0.1
1,547,398 - Pf6N2E2_1313 0.15 +0.7
1,547,856 + Pf6N2E2_1313 0.72 +0.1
1,547,856 + Pf6N2E2_1313 0.72 -1.2
1,547,953 - Pf6N2E2_1313 0.84 +0.2
1,548,182 + Pf6N2E2_1314 0.11 +2.1

Or see this region's nucleotide sequence