Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00430 and HSERO_RS00435 are separated by 38 nucleotides HSERO_RS00435 and HSERO_RS00440 are separated by 26 nucleotides HSERO_RS00440 and HSERO_RS00445 are separated by 53 nucleotides
HSERO_RS00430: HSERO_RS00430 - glutamate--cysteine ligase, at 101,286 to 102,587
_RS00430
HSERO_RS00435: HSERO_RS00435 - glutathione synthetase, at 102,626 to 103,576
_RS00435
HSERO_RS00440: HSERO_RS00440 - PTS fructose transporter subunit IIA, at 103,603 to 104,079
_RS00440
HSERO_RS00445: HSERO_RS00445 - phosphocarrier protein HPr, at 104,133 to 104,402
_RS00445
Position (kb) 102
103
104 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 101.739 kb on - strand, within HSERO_RS00430 at 101.773 kb on + strand, within HSERO_RS00430 at 102.126 kb on + strand, within HSERO_RS00430 at 102.256 kb on + strand, within HSERO_RS00430 at 102.559 kb on + strand at 102.608 kb on + strand at 102.613 kb on - strand at 102.615 kb on + strand at 102.616 kb on - strand at 102.661 kb on + strand at 102.843 kb on + strand, within HSERO_RS00435 at 102.843 kb on + strand, within HSERO_RS00435 at 103.248 kb on + strand, within HSERO_RS00435 at 103.249 kb on - strand, within HSERO_RS00435 at 103.455 kb on + strand, within HSERO_RS00435 at 103.580 kb on - strand at 103.605 kb on + strand at 104.335 kb on + strand, within HSERO_RS00445 at 104.460 kb on - strand at 104.529 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 101,739 - HSERO_RS00430 0.35 +0.8 101,773 + HSERO_RS00430 0.37 +0.8 102,126 + HSERO_RS00430 0.65 -0.5 102,256 + HSERO_RS00430 0.75 +0.8 102,559 + +0.8 102,608 + +0.3 102,613 - -0.2 102,615 + -0.3 102,616 - -0.2 102,661 + -0.0 102,843 + HSERO_RS00435 0.23 -2.5 102,843 + HSERO_RS00435 0.23 -1.4 103,248 + HSERO_RS00435 0.65 -2.3 103,249 - HSERO_RS00435 0.66 -3.0 103,455 + HSERO_RS00435 0.87 -0.2 103,580 - +0.3 103,605 + +0.2 104,335 + HSERO_RS00445 0.75 +1.3 104,460 - -1.0 104,529 + +0.7
Or see this region's nucleotide sequence