Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01404 and SMc01493 are separated by 113 nucleotides SMc01493 and SMc01405 are separated by 164 nucleotides
SMc01404: SMc01404 - aspartate racemase, at 2,245,222 to 2,245,926
SMc01404
SMc01493: SMc01493 - hypothetical protein, at 2,246,040 to 2,246,510
SMc01493
SMc01405: SMc01405 - hypothetical protein, at 2,246,675 to 2,247,562
SMc01405
Position (kb)
2246
2247 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2245.042 kb on + strand at 2245.094 kb on + strand at 2245.149 kb on - strand at 2245.153 kb on + strand at 2245.154 kb on - strand at 2245.279 kb on + strand at 2245.279 kb on + strand at 2245.279 kb on + strand at 2245.279 kb on + strand at 2245.279 kb on + strand at 2245.279 kb on + strand at 2245.280 kb on - strand at 2245.280 kb on - strand at 2245.280 kb on - strand at 2245.280 kb on - strand at 2245.325 kb on + strand at 2245.326 kb on - strand, within SMc01404 at 2245.606 kb on + strand, within SMc01404 at 2245.627 kb on + strand, within SMc01404 at 2245.628 kb on - strand, within SMc01404 at 2245.628 kb on - strand, within SMc01404 at 2245.654 kb on - strand, within SMc01404 at 2245.667 kb on + strand, within SMc01404 at 2245.925 kb on - strand at 2245.925 kb on - strand at 2245.949 kb on + strand at 2245.951 kb on + strand at 2245.952 kb on - strand at 2245.952 kb on - strand at 2245.952 kb on - strand at 2245.952 kb on - strand at 2245.952 kb on - strand at 2245.952 kb on - strand at 2246.123 kb on - strand, within SMc01493 at 2246.145 kb on - strand, within SMc01493 at 2246.258 kb on + strand, within SMc01493 at 2246.270 kb on - strand, within SMc01493 at 2246.399 kb on + strand, within SMc01493 at 2246.399 kb on + strand, within SMc01493 at 2246.399 kb on + strand, within SMc01493 at 2246.400 kb on - strand, within SMc01493 at 2246.400 kb on - strand, within SMc01493 at 2246.408 kb on + strand, within SMc01493 at 2246.555 kb on + strand at 2246.564 kb on + strand at 2246.771 kb on - strand, within SMc01405 at 2246.773 kb on + strand, within SMc01405 at 2246.774 kb on - strand, within SMc01405 at 2246.774 kb on - strand, within SMc01405 at 2246.810 kb on + strand, within SMc01405 at 2246.811 kb on - strand, within SMc01405 at 2246.895 kb on + strand, within SMc01405 at 2247.000 kb on - strand, within SMc01405 at 2247.001 kb on + strand, within SMc01405 at 2247.001 kb on + strand, within SMc01405 at 2247.001 kb on + strand, within SMc01405 at 2247.002 kb on - strand, within SMc01405 at 2247.002 kb on - strand, within SMc01405 at 2247.092 kb on + strand, within SMc01405 at 2247.120 kb on + strand, within SMc01405 at 2247.194 kb on - strand, within SMc01405 at 2247.272 kb on + strand, within SMc01405 at 2247.272 kb on + strand, within SMc01405 at 2247.272 kb on + strand, within SMc01405 at 2247.273 kb on - strand, within SMc01405 at 2247.273 kb on - strand, within SMc01405 at 2247.273 kb on - strand, within SMc01405 at 2247.273 kb on - strand, within SMc01405 at 2247.273 kb on - strand, within SMc01405 at 2247.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 2,245,042 + +0.3 2,245,094 + -0.4 2,245,149 - +0.2 2,245,153 + -0.4 2,245,154 - -0.2 2,245,279 + -0.4 2,245,279 + -2.5 2,245,279 + -1.1 2,245,279 + -0.7 2,245,279 + +0.4 2,245,279 + +0.6 2,245,280 - -1.5 2,245,280 - -1.6 2,245,280 - -0.1 2,245,280 - -1.2 2,245,325 + -1.0 2,245,326 - SMc01404 0.15 -1.5 2,245,606 + SMc01404 0.54 -0.6 2,245,627 + SMc01404 0.57 +1.3 2,245,628 - SMc01404 0.58 -0.7 2,245,628 - SMc01404 0.58 -0.9 2,245,654 - SMc01404 0.61 -0.8 2,245,667 + SMc01404 0.63 -0.2 2,245,925 - -3.0 2,245,925 - -1.6 2,245,949 + +1.7 2,245,951 + -1.7 2,245,952 - -0.5 2,245,952 - +0.6 2,245,952 - +1.8 2,245,952 - -1.7 2,245,952 - +1.9 2,245,952 - -1.2 2,246,123 - SMc01493 0.18 -0.2 2,246,145 - SMc01493 0.22 -1.2 2,246,258 + SMc01493 0.46 +0.5 2,246,270 - SMc01493 0.49 +1.2 2,246,399 + SMc01493 0.76 +1.5 2,246,399 + SMc01493 0.76 +0.1 2,246,399 + SMc01493 0.76 +1.1 2,246,400 - SMc01493 0.76 -0.1 2,246,400 - SMc01493 0.76 -0.3 2,246,408 + SMc01493 0.78 +1.5 2,246,555 + +0.3 2,246,564 + -0.2 2,246,771 - SMc01405 0.11 -0.0 2,246,773 + SMc01405 0.11 -0.5 2,246,774 - SMc01405 0.11 -0.7 2,246,774 - SMc01405 0.11 -0.6 2,246,810 + SMc01405 0.15 -0.2 2,246,811 - SMc01405 0.15 -0.6 2,246,895 + SMc01405 0.25 +0.3 2,247,000 - SMc01405 0.37 +0.0 2,247,001 + SMc01405 0.37 -0.8 2,247,001 + SMc01405 0.37 -1.5 2,247,001 + SMc01405 0.37 -2.4 2,247,002 - SMc01405 0.37 -1.7 2,247,002 - SMc01405 0.37 +0.2 2,247,092 + SMc01405 0.47 -1.4 2,247,120 + SMc01405 0.50 -0.8 2,247,194 - SMc01405 0.58 +0.0 2,247,272 + SMc01405 0.67 -0.5 2,247,272 + SMc01405 0.67 +0.9 2,247,272 + SMc01405 0.67 -0.3 2,247,273 - SMc01405 0.67 -1.0 2,247,273 - SMc01405 0.67 +0.7 2,247,273 - SMc01405 0.67 -0.9 2,247,273 - SMc01405 0.67 -1.4 2,247,273 - SMc01405 0.67 +2.1 2,247,475 - -0.8
Or see this region's nucleotide sequence