Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12115 and HSERO_RS12120 overlap by 4 nucleotides HSERO_RS12120 and HSERO_RS12125 are separated by 10 nucleotides HSERO_RS12125 and HSERO_RS12130 overlap by 4 nucleotides
HSERO_RS12115: HSERO_RS12115 - ATPase, at 2,751,737 to 2,752,876
_RS12115
HSERO_RS12120: HSERO_RS12120 - ABC transporter ATP-binding protein, at 2,752,873 to 2,753,673
_RS12120
HSERO_RS12125: HSERO_RS12125 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data) , at 2,753,684 to 2,755,657
_RS12125
HSERO_RS12130: HSERO_RS12130 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (from data) , at 2,755,654 to 2,757,531
_RS12130
Position (kb)
2753
2754
2755
2756 Strain fitness (log2 ratio)
-2
-1
0
1 at 2752.770 kb on + strand at 2752.894 kb on + strand at 2752.923 kb on - strand at 2752.928 kb on - strand at 2753.022 kb on + strand, within HSERO_RS12120 at 2753.040 kb on + strand, within HSERO_RS12120 at 2753.076 kb on - strand, within HSERO_RS12120 at 2753.117 kb on - strand, within HSERO_RS12120 at 2753.291 kb on + strand, within HSERO_RS12120 at 2753.506 kb on - strand, within HSERO_RS12120 at 2753.534 kb on + strand, within HSERO_RS12120 at 2753.534 kb on + strand, within HSERO_RS12120 at 2753.542 kb on - strand, within HSERO_RS12120 at 2753.816 kb on + strand at 2753.986 kb on - strand, within HSERO_RS12125 at 2754.034 kb on + strand, within HSERO_RS12125 at 2754.126 kb on + strand at 2754.133 kb on + strand, within HSERO_RS12125 at 2754.183 kb on + strand, within HSERO_RS12125 at 2754.248 kb on + strand, within HSERO_RS12125 at 2754.395 kb on + strand, within HSERO_RS12125 at 2754.448 kb on + strand, within HSERO_RS12125 at 2754.476 kb on + strand, within HSERO_RS12125 at 2754.484 kb on - strand, within HSERO_RS12125 at 2754.552 kb on - strand, within HSERO_RS12125 at 2754.624 kb on + strand, within HSERO_RS12125 at 2754.648 kb on - strand, within HSERO_RS12125 at 2754.695 kb on - strand, within HSERO_RS12125 at 2755.192 kb on - strand, within HSERO_RS12125 at 2755.192 kb on - strand, within HSERO_RS12125 at 2755.192 kb on - strand, within HSERO_RS12125 at 2755.301 kb on - strand, within HSERO_RS12125 at 2755.323 kb on + strand, within HSERO_RS12125 at 2756.188 kb on - strand, within HSERO_RS12130 at 2756.402 kb on - strand, within HSERO_RS12130 at 2756.479 kb on + strand, within HSERO_RS12130 at 2756.612 kb on + strand, within HSERO_RS12130
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 2,752,770 + +0.8 2,752,894 + -2.2 2,752,923 - -0.1 2,752,928 - -0.2 2,753,022 + HSERO_RS12120 0.19 +0.1 2,753,040 + HSERO_RS12120 0.21 -0.2 2,753,076 - HSERO_RS12120 0.25 +0.2 2,753,117 - HSERO_RS12120 0.30 +0.4 2,753,291 + HSERO_RS12120 0.52 -1.1 2,753,506 - HSERO_RS12120 0.79 -1.5 2,753,534 + HSERO_RS12120 0.83 -0.4 2,753,534 + HSERO_RS12120 0.83 -1.1 2,753,542 - HSERO_RS12120 0.84 +1.1 2,753,816 + +0.1 2,753,986 - HSERO_RS12125 0.15 -0.1 2,754,034 + HSERO_RS12125 0.18 +0.2 2,754,126 + -0.1 2,754,133 + HSERO_RS12125 0.23 -0.5 2,754,183 + HSERO_RS12125 0.25 -0.1 2,754,248 + HSERO_RS12125 0.29 +0.1 2,754,395 + HSERO_RS12125 0.36 +0.3 2,754,448 + HSERO_RS12125 0.39 +0.6 2,754,476 + HSERO_RS12125 0.40 -0.1 2,754,484 - HSERO_RS12125 0.41 -0.3 2,754,552 - HSERO_RS12125 0.44 +0.5 2,754,624 + HSERO_RS12125 0.48 +0.3 2,754,648 - HSERO_RS12125 0.49 -0.5 2,754,695 - HSERO_RS12125 0.51 +0.2 2,755,192 - HSERO_RS12125 0.76 -0.5 2,755,192 - HSERO_RS12125 0.76 -0.6 2,755,192 - HSERO_RS12125 0.76 -0.1 2,755,301 - HSERO_RS12125 0.82 -0.4 2,755,323 + HSERO_RS12125 0.83 +0.1 2,756,188 - HSERO_RS12130 0.28 -0.2 2,756,402 - HSERO_RS12130 0.40 -0.9 2,756,479 + HSERO_RS12130 0.44 +0.5 2,756,612 + HSERO_RS12130 0.51 -1.5
Or see this region's nucleotide sequence