Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05240 and HSERO_RS05245 are separated by 7 nucleotides HSERO_RS05245 and HSERO_RS05250 are separated by 158 nucleotides HSERO_RS05250 and HSERO_RS05255 are separated by 37 nucleotides
HSERO_RS05240: HSERO_RS05240 - short-chain dehydrogenase, at 1,160,755 to 1,161,531
_RS05240
HSERO_RS05245: HSERO_RS05245 - hypothetical protein, at 1,161,539 to 1,161,901
_RS05245
HSERO_RS05250: HSERO_RS05250 - ABC transporter for L-fucose, ATPase component (from data) , at 1,162,060 to 1,163,622
_RS05250
HSERO_RS05255: HSERO_RS05255 - ABC transporter for L-fucose, permease component (from data) , at 1,163,660 to 1,164,703
_RS05255
Position (kb)
1162
1163
1164 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1161.115 kb on + strand, within HSERO_RS05240 at 1161.234 kb on + strand, within HSERO_RS05240 at 1161.245 kb on + strand, within HSERO_RS05240 at 1161.411 kb on - strand, within HSERO_RS05240 at 1161.719 kb on + strand, within HSERO_RS05245 at 1161.782 kb on + strand, within HSERO_RS05245 at 1161.850 kb on - strand, within HSERO_RS05245 at 1161.976 kb on + strand at 1162.054 kb on - strand at 1162.445 kb on - strand, within HSERO_RS05250 at 1162.586 kb on + strand, within HSERO_RS05250 at 1162.613 kb on - strand, within HSERO_RS05250 at 1162.694 kb on - strand, within HSERO_RS05250 at 1162.698 kb on + strand, within HSERO_RS05250 at 1162.715 kb on + strand, within HSERO_RS05250 at 1162.889 kb on + strand, within HSERO_RS05250 at 1162.960 kb on + strand, within HSERO_RS05250 at 1163.049 kb on - strand, within HSERO_RS05250 at 1163.171 kb on + strand, within HSERO_RS05250 at 1163.174 kb on - strand, within HSERO_RS05250 at 1163.459 kb on - strand, within HSERO_RS05250 at 1163.459 kb on - strand, within HSERO_RS05250 at 1163.474 kb on + strand at 1163.721 kb on + strand at 1163.729 kb on - strand at 1163.776 kb on - strand, within HSERO_RS05255 at 1163.834 kb on + strand, within HSERO_RS05255 at 1163.944 kb on - strand, within HSERO_RS05255 at 1164.084 kb on - strand, within HSERO_RS05255 at 1164.121 kb on + strand at 1164.129 kb on - strand, within HSERO_RS05255 at 1164.213 kb on + strand, within HSERO_RS05255 at 1164.334 kb on - strand, within HSERO_RS05255 at 1164.336 kb on + strand, within HSERO_RS05255 at 1164.582 kb on - strand, within HSERO_RS05255
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 1,161,115 + HSERO_RS05240 0.46 -0.6 1,161,234 + HSERO_RS05240 0.62 -0.1 1,161,245 + HSERO_RS05240 0.63 -0.5 1,161,411 - HSERO_RS05240 0.84 -0.4 1,161,719 + HSERO_RS05245 0.50 +1.1 1,161,782 + HSERO_RS05245 0.67 -0.6 1,161,850 - HSERO_RS05245 0.86 -0.1 1,161,976 + -0.0 1,162,054 - -0.0 1,162,445 - HSERO_RS05250 0.25 +0.2 1,162,586 + HSERO_RS05250 0.34 +1.2 1,162,613 - HSERO_RS05250 0.35 +0.3 1,162,694 - HSERO_RS05250 0.41 +1.2 1,162,698 + HSERO_RS05250 0.41 +0.1 1,162,715 + HSERO_RS05250 0.42 -0.2 1,162,889 + HSERO_RS05250 0.53 +2.1 1,162,960 + HSERO_RS05250 0.58 +1.2 1,163,049 - HSERO_RS05250 0.63 -0.4 1,163,171 + HSERO_RS05250 0.71 -0.1 1,163,174 - HSERO_RS05250 0.71 -0.5 1,163,459 - HSERO_RS05250 0.90 -0.1 1,163,459 - HSERO_RS05250 0.90 +2.4 1,163,474 + +0.4 1,163,721 + -1.5 1,163,729 - -1.4 1,163,776 - HSERO_RS05255 0.11 +3.3 1,163,834 + HSERO_RS05255 0.17 -0.0 1,163,944 - HSERO_RS05255 0.27 -0.3 1,164,084 - HSERO_RS05255 0.41 -0.2 1,164,121 + +0.8 1,164,129 - HSERO_RS05255 0.45 -0.0 1,164,213 + HSERO_RS05255 0.53 -0.7 1,164,334 - HSERO_RS05255 0.65 -0.2 1,164,336 + HSERO_RS05255 0.65 +0.4 1,164,582 - HSERO_RS05255 0.88 +0.0
Or see this region's nucleotide sequence