Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1302 and SMa1303 are separated by 10 nucleotides SMa1303 and SMa1306 are separated by 9 nucleotides SMa1306 and SMa1308 overlap by 1 nucleotides
SMa1302: SMa1302 - VirB11-like ATPase, at 711,238 to 712,227
SMa1302
SMa1303: SMa1303 - VirB10-like transmembrane secretion protein, at 712,238 to 713,410
SMa1303
SMa1306: SMa1306 - VirB9 type IV secretion protein, at 713,420 to 714,274
SMa1306
SMa1308: SMa1308 - VirB8 type IV secretion protein, at 714,274 to 714,945
SMa1308
Position (kb) 712
713
714 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 711.252 kb on - strand at 711.301 kb on + strand at 711.301 kb on + strand at 711.313 kb on + strand at 711.314 kb on - strand at 711.426 kb on + strand, within SMa1302 at 711.427 kb on - strand, within SMa1302 at 711.431 kb on - strand, within SMa1302 at 711.431 kb on - strand, within SMa1302 at 711.468 kb on - strand, within SMa1302 at 711.470 kb on + strand, within SMa1302 at 711.470 kb on + strand, within SMa1302 at 711.471 kb on - strand, within SMa1302 at 711.471 kb on - strand, within SMa1302 at 711.588 kb on - strand, within SMa1302 at 711.592 kb on + strand, within SMa1302 at 711.593 kb on - strand, within SMa1302 at 711.593 kb on - strand, within SMa1302 at 711.619 kb on - strand, within SMa1302 at 711.789 kb on - strand, within SMa1302 at 711.789 kb on - strand, within SMa1302 at 712.085 kb on + strand, within SMa1302 at 712.086 kb on - strand, within SMa1302 at 712.135 kb on + strand at 712.135 kb on + strand at 712.148 kb on + strand at 712.240 kb on - strand at 712.240 kb on - strand at 712.329 kb on + strand at 712.329 kb on + strand at 712.330 kb on - strand at 712.330 kb on - strand at 712.330 kb on - strand at 712.506 kb on + strand, within SMa1303 at 712.506 kb on + strand, within SMa1303 at 712.507 kb on - strand, within SMa1303 at 712.578 kb on + strand, within SMa1303 at 712.578 kb on + strand, within SMa1303 at 712.711 kb on - strand, within SMa1303 at 712.838 kb on - strand, within SMa1303 at 713.062 kb on + strand, within SMa1303 at 713.064 kb on + strand, within SMa1303 at 713.065 kb on - strand, within SMa1303 at 713.199 kb on - strand, within SMa1303 at 713.422 kb on - strand at 713.422 kb on - strand at 713.479 kb on + strand at 713.483 kb on - strand at 713.508 kb on + strand, within SMa1306 at 713.518 kb on + strand, within SMa1306 at 713.576 kb on + strand, within SMa1306 at 713.643 kb on + strand, within SMa1306 at 713.643 kb on + strand, within SMa1306 at 713.643 kb on + strand, within SMa1306 at 713.644 kb on - strand, within SMa1306 at 713.644 kb on - strand, within SMa1306 at 713.703 kb on + strand, within SMa1306 at 713.766 kb on + strand, within SMa1306 at 713.766 kb on + strand, within SMa1306 at 713.874 kb on + strand, within SMa1306 at 713.884 kb on - strand, within SMa1306 at 713.889 kb on + strand, within SMa1306 at 713.944 kb on - strand, within SMa1306 at 713.947 kb on + strand, within SMa1306 at 713.987 kb on - strand, within SMa1306 at 714.153 kb on + strand, within SMa1306 at 714.154 kb on - strand, within SMa1306 at 714.162 kb on + strand, within SMa1306 at 714.162 kb on + strand, within SMa1306 at 714.163 kb on - strand, within SMa1306 at 714.163 kb on - strand, within SMa1306 at 714.266 kb on - strand at 714.368 kb on + strand, within SMa1308
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 711,252 - -0.9 711,301 + -1.1 711,301 + -2.6 711,313 + -0.1 711,314 - +0.3 711,426 + SMa1302 0.19 -1.1 711,427 - SMa1302 0.19 -1.0 711,431 - SMa1302 0.19 -1.8 711,431 - SMa1302 0.19 +0.3 711,468 - SMa1302 0.23 +0.9 711,470 + SMa1302 0.23 -0.7 711,470 + SMa1302 0.23 +0.7 711,471 - SMa1302 0.24 +1.0 711,471 - SMa1302 0.24 -0.2 711,588 - SMa1302 0.35 -0.7 711,592 + SMa1302 0.36 -1.6 711,593 - SMa1302 0.36 +1.9 711,593 - SMa1302 0.36 +0.8 711,619 - SMa1302 0.38 -0.9 711,789 - SMa1302 0.56 -0.1 711,789 - SMa1302 0.56 +0.2 712,085 + SMa1302 0.86 -0.3 712,086 - SMa1302 0.86 +0.7 712,135 + +0.6 712,135 + +1.2 712,148 + -2.2 712,240 - +1.3 712,240 - +1.5 712,329 + +0.0 712,329 + -2.2 712,330 - +0.8 712,330 - +0.0 712,330 - +1.6 712,506 + SMa1303 0.23 +0.2 712,506 + SMa1303 0.23 -1.5 712,507 - SMa1303 0.23 -1.4 712,578 + SMa1303 0.29 +0.3 712,578 + SMa1303 0.29 -2.9 712,711 - SMa1303 0.40 -1.1 712,838 - SMa1303 0.51 -1.1 713,062 + SMa1303 0.70 +0.4 713,064 + SMa1303 0.70 -1.2 713,065 - SMa1303 0.71 -0.8 713,199 - SMa1303 0.82 -0.8 713,422 - -0.8 713,422 - -0.0 713,479 + -2.8 713,483 - -1.2 713,508 + SMa1306 0.10 -0.3 713,518 + SMa1306 0.11 +0.8 713,576 + SMa1306 0.18 +0.7 713,643 + SMa1306 0.26 -2.7 713,643 + SMa1306 0.26 +0.9 713,643 + SMa1306 0.26 -0.7 713,644 - SMa1306 0.26 +1.3 713,644 - SMa1306 0.26 +0.9 713,703 + SMa1306 0.33 -0.0 713,766 + SMa1306 0.40 +0.6 713,766 + SMa1306 0.40 +0.6 713,874 + SMa1306 0.53 +0.7 713,884 - SMa1306 0.54 -0.1 713,889 + SMa1306 0.55 -0.3 713,944 - SMa1306 0.61 -0.2 713,947 + SMa1306 0.62 +0.7 713,987 - SMa1306 0.66 -0.3 714,153 + SMa1306 0.86 +0.0 714,154 - SMa1306 0.86 +1.3 714,162 + SMa1306 0.87 +1.9 714,162 + SMa1306 0.87 +1.0 714,163 - SMa1306 0.87 -0.3 714,163 - SMa1306 0.87 -1.0 714,266 - -0.4 714,368 + SMa1308 0.14 -0.2
Or see this region's nucleotide sequence