Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20219 and SM_b22002 are separated by 91 nucleotides SM_b22002 and SM_b20221 overlap by 124 nucleotides SM_b20221 and SM_b20222 are separated by 9 nucleotides
SM_b20219: SM_b20219 - response regulator protein, at 227,578 to 228,315
_b20219
SM_b22002: SM_b22002 - epoxide hydrolase, at 228,407 to 228,829
_b22002
SM_b20221: SM_b20221 - epoxide hydrolase, at 228,706 to 229,740
_b20221
SM_b20222: SM_b20222 - hypothetical protein, at 229,750 to 230,172
_b20222
Position (kb) 228
229
230 Strain fitness (log2 ratio) -2
-1
0
1
2 at 227.839 kb on - strand, within SM_b20219 at 228.006 kb on + strand, within SM_b20219 at 228.006 kb on + strand, within SM_b20219 at 228.007 kb on - strand, within SM_b20219 at 228.009 kb on - strand, within SM_b20219 at 228.174 kb on + strand at 228.249 kb on - strand at 228.249 kb on - strand at 228.373 kb on + strand at 228.373 kb on + strand at 228.374 kb on - strand at 228.501 kb on + strand, within SM_b22002 at 228.504 kb on + strand at 228.552 kb on + strand, within SM_b22002 at 228.553 kb on - strand, within SM_b22002 at 228.672 kb on + strand, within SM_b22002 at 228.710 kb on + strand at 228.759 kb on + strand at 228.759 kb on + strand at 228.960 kb on + strand, within SM_b20221 at 228.961 kb on - strand, within SM_b20221 at 229.063 kb on + strand, within SM_b20221 at 229.356 kb on + strand, within SM_b20221 at 229.356 kb on + strand, within SM_b20221 at 229.412 kb on + strand, within SM_b20221 at 229.791 kb on + strand at 229.791 kb on + strand at 229.792 kb on - strand at 229.792 kb on - strand at 229.792 kb on - strand at 229.792 kb on - strand at 229.793 kb on + strand, within SM_b20222 at 229.793 kb on + strand, within SM_b20222 at 229.793 kb on + strand, within SM_b20222 at 229.794 kb on - strand, within SM_b20222 at 229.794 kb on - strand, within SM_b20222 at 229.937 kb on + strand, within SM_b20222 at 229.937 kb on + strand, within SM_b20222 at 229.937 kb on + strand, within SM_b20222 at 229.938 kb on - strand, within SM_b20222 at 229.938 kb on - strand, within SM_b20222 at 229.938 kb on - strand, within SM_b20222 at 230.152 kb on - strand at 230.152 kb on - strand at 230.205 kb on + strand at 230.223 kb on + strand at 230.223 kb on + strand at 230.224 kb on - strand at 230.224 kb on - strand at 230.541 kb on + strand at 230.541 kb on + strand at 230.542 kb on - strand at 230.686 kb on + strand at 230.687 kb on - strand at 230.725 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 227,839 - SM_b20219 0.35 -2.1 228,006 + SM_b20219 0.58 -2.6 228,006 + SM_b20219 0.58 +0.4 228,007 - SM_b20219 0.58 +0.0 228,009 - SM_b20219 0.58 +0.1 228,174 + -1.1 228,249 - -0.6 228,249 - +0.5 228,373 + -0.7 228,373 + -0.5 228,374 - +0.5 228,501 + SM_b22002 0.22 +0.2 228,504 + +0.0 228,552 + SM_b22002 0.34 -0.3 228,553 - SM_b22002 0.35 +0.6 228,672 + SM_b22002 0.63 -0.3 228,710 + +0.6 228,759 + +0.3 228,759 + +1.4 228,960 + SM_b20221 0.25 +0.9 228,961 - SM_b20221 0.25 +0.6 229,063 + SM_b20221 0.34 +0.3 229,356 + SM_b20221 0.63 +2.2 229,356 + SM_b20221 0.63 +0.5 229,412 + SM_b20221 0.68 +0.6 229,791 + +0.6 229,791 + -1.5 229,792 - -2.0 229,792 - -0.7 229,792 - -1.4 229,792 - +1.0 229,793 + SM_b20222 0.10 +0.3 229,793 + SM_b20222 0.10 -2.5 229,793 + SM_b20222 0.10 +0.2 229,794 - SM_b20222 0.10 -1.1 229,794 - SM_b20222 0.10 -2.0 229,937 + SM_b20222 0.44 -0.7 229,937 + SM_b20222 0.44 +0.4 229,937 + SM_b20222 0.44 +0.3 229,938 - SM_b20222 0.44 -0.5 229,938 - SM_b20222 0.44 -1.0 229,938 - SM_b20222 0.44 -0.3 230,152 - +1.2 230,152 - +0.8 230,205 + -0.2 230,223 + -2.0 230,223 + -0.4 230,224 - -0.2 230,224 - -0.2 230,541 + +0.0 230,541 + -1.1 230,542 - +0.1 230,686 + -0.6 230,687 - -2.7 230,725 + -1.5
Or see this region's nucleotide sequence