Experiment: L-Glutamine (C)(N); with TAPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01660 and PFLU_RS01665 are separated by 1 nucleotides PFLU_RS01665 and PFLU_RS01670 are separated by 39 nucleotides PFLU_RS01670 and PFLU_RS01675 are separated by 119 nucleotides PFLU_RS01675 and PFLU_RS01680 overlap by 2 nucleotides PFLU_RS01680 and PFLU_RS30350 are separated by 139 nucleotides PFLU_RS30350 and PFLU_RS01685 are separated by 81 nucleotides
PFLU_RS01660: PFLU_RS01660 - rhodanese-like domain-containing protein, at 371,474 to 371,887
_RS01660
PFLU_RS01665: PFLU_RS01665 - glutaredoxin 3, at 371,889 to 372,143
_RS01665
PFLU_RS01670: PFLU_RS01670 - protein-export chaperone SecB, at 372,183 to 372,662
_RS01670
PFLU_RS01675: PFLU_RS01675 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 372,782 to 373,237
_RS01675
PFLU_RS01680: PFLU_RS01680 - hypothetical protein, at 373,236 to 373,676
_RS01680
PFLU_RS30350: PFLU_RS30350 - hypothetical protein, at 373,816 to 374,127
_RS30350
PFLU_RS01685: PFLU_RS01685 - nitrogen regulation protein NR(I), at 374,209 to 375,645
_RS01685
Position (kb) 372
373
374 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 371.866 kb on + strand at 371.888 kb on + strand at 371.888 kb on + strand at 371.888 kb on + strand at 371.888 kb on + strand at 371.888 kb on + strand at 371.888 kb on + strand at 371.888 kb on + strand at 371.889 kb on - strand at 371.889 kb on - strand at 371.889 kb on - strand at 371.889 kb on - strand at 371.889 kb on - strand at 371.889 kb on - strand at 371.889 kb on - strand at 371.889 kb on - strand at 371.889 kb on - strand at 371.889 kb on - strand at 371.907 kb on + strand at 371.908 kb on - strand at 371.908 kb on - strand at 371.908 kb on - strand at 371.908 kb on - strand at 371.928 kb on + strand, within PFLU_RS01665 at 371.928 kb on + strand, within PFLU_RS01665 at 371.929 kb on - strand, within PFLU_RS01665 at 371.929 kb on - strand, within PFLU_RS01665 at 371.929 kb on - strand, within PFLU_RS01665 at 371.929 kb on - strand, within PFLU_RS01665 at 372.155 kb on + strand at 372.155 kb on + strand at 372.155 kb on + strand at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.249 kb on + strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.250 kb on - strand, within PFLU_RS01670 at 372.447 kb on - strand, within PFLU_RS01670 at 372.526 kb on - strand, within PFLU_RS01670 at 372.526 kb on - strand, within PFLU_RS01670 at 372.660 kb on + strand at 372.660 kb on + strand at 372.660 kb on + strand at 372.660 kb on + strand at 372.660 kb on + strand at 372.660 kb on + strand at 372.660 kb on + strand at 372.661 kb on - strand at 372.661 kb on - strand at 372.661 kb on - strand at 372.661 kb on - strand at 372.661 kb on - strand at 372.661 kb on - strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.721 kb on + strand at 372.775 kb on + strand at 372.776 kb on - strand at 372.783 kb on + strand at 372.783 kb on + strand at 372.783 kb on + strand at 372.783 kb on + strand at 372.784 kb on - strand at 372.784 kb on - strand at 372.784 kb on - strand at 372.784 kb on - strand at 372.784 kb on - strand at 372.813 kb on + strand at 372.813 kb on + strand at 372.813 kb on + strand at 372.813 kb on + strand at 372.813 kb on + strand at 372.813 kb on + strand at 372.813 kb on + strand at 372.814 kb on - strand at 372.814 kb on - strand at 372.814 kb on - strand at 372.814 kb on - strand at 372.908 kb on + strand, within PFLU_RS01675 at 372.908 kb on + strand, within PFLU_RS01675 at 372.909 kb on - strand, within PFLU_RS01675 at 372.909 kb on - strand, within PFLU_RS01675 at 373.078 kb on - strand, within PFLU_RS01675 at 373.087 kb on - strand, within PFLU_RS01675 at 373.183 kb on + strand, within PFLU_RS01675 at 373.271 kb on + strand at 373.300 kb on + strand, within PFLU_RS01680 at 373.300 kb on + strand, within PFLU_RS01680 at 373.300 kb on + strand, within PFLU_RS01680 at 373.300 kb on + strand, within PFLU_RS01680 at 373.300 kb on + strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.486 kb on + strand, within PFLU_RS01680 at 373.487 kb on - strand, within PFLU_RS01680 at 373.487 kb on - strand, within PFLU_RS01680 at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.730 kb on - strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.760 kb on + strand at 373.760 kb on + strand at 373.760 kb on + strand at 373.760 kb on + strand at 373.761 kb on - strand at 373.761 kb on - strand at 373.761 kb on - strand at 373.787 kb on + strand at 373.787 kb on + strand at 373.832 kb on + strand at 373.858 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 374.010 kb on + strand, within PFLU_RS30350 at 374.011 kb on - strand, within PFLU_RS30350 at 374.011 kb on - strand, within PFLU_RS30350 at 374.011 kb on - strand, within PFLU_RS30350 at 374.011 kb on - strand, within PFLU_RS30350 at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C)(N); with TAPS remove 371,866 + +0.0 371,888 + -0.3 371,888 + -0.9 371,888 + +0.1 371,888 + +1.1 371,888 + +1.7 371,888 + -2.0 371,888 + -1.8 371,889 - -2.3 371,889 - +2.1 371,889 - +1.5 371,889 - -0.3 371,889 - -0.0 371,889 - -3.0 371,889 - -1.3 371,889 - -0.0 371,889 - +1.3 371,889 - -0.0 371,907 + -1.0 371,908 - +1.3 371,908 - -0.5 371,908 - +0.4 371,908 - -1.2 371,928 + PFLU_RS01665 0.15 +0.5 371,928 + PFLU_RS01665 0.15 +0.9 371,929 - PFLU_RS01665 0.16 -0.8 371,929 - PFLU_RS01665 0.16 +0.1 371,929 - PFLU_RS01665 0.16 +0.2 371,929 - PFLU_RS01665 0.16 +1.4 372,155 + -1.2 372,155 + -1.6 372,155 + +0.3 372,249 + PFLU_RS01670 0.14 -1.0 372,249 + PFLU_RS01670 0.14 -1.2 372,249 + PFLU_RS01670 0.14 -0.7 372,249 + PFLU_RS01670 0.14 -2.5 372,249 + PFLU_RS01670 0.14 -1.5 372,249 + PFLU_RS01670 0.14 -2.3 372,249 + PFLU_RS01670 0.14 +1.3 372,249 + PFLU_RS01670 0.14 -0.5 372,249 + PFLU_RS01670 0.14 -1.0 372,249 + PFLU_RS01670 0.14 +0.5 372,249 + PFLU_RS01670 0.14 -0.5 372,249 + PFLU_RS01670 0.14 -0.4 372,249 + PFLU_RS01670 0.14 +2.1 372,249 + PFLU_RS01670 0.14 +0.4 372,249 + PFLU_RS01670 0.14 +0.1 372,249 + PFLU_RS01670 0.14 +0.3 372,249 + PFLU_RS01670 0.14 -2.9 372,249 + PFLU_RS01670 0.14 -0.7 372,249 + PFLU_RS01670 0.14 -0.6 372,249 + PFLU_RS01670 0.14 -1.3 372,249 + PFLU_RS01670 0.14 -1.6 372,249 + PFLU_RS01670 0.14 -2.3 372,249 + PFLU_RS01670 0.14 -0.7 372,250 - PFLU_RS01670 0.14 -0.6 372,250 - PFLU_RS01670 0.14 -1.6 372,250 - PFLU_RS01670 0.14 -1.5 372,250 - PFLU_RS01670 0.14 -1.0 372,250 - PFLU_RS01670 0.14 -0.3 372,250 - PFLU_RS01670 0.14 +0.5 372,250 - PFLU_RS01670 0.14 -0.9 372,250 - PFLU_RS01670 0.14 -1.0 372,250 - PFLU_RS01670 0.14 +1.5 372,250 - PFLU_RS01670 0.14 +1.4 372,250 - PFLU_RS01670 0.14 -1.6 372,250 - PFLU_RS01670 0.14 -1.0 372,250 - PFLU_RS01670 0.14 -0.8 372,250 - PFLU_RS01670 0.14 -1.0 372,250 - PFLU_RS01670 0.14 +3.1 372,250 - PFLU_RS01670 0.14 -0.7 372,250 - PFLU_RS01670 0.14 -0.2 372,250 - PFLU_RS01670 0.14 +0.9 372,250 - PFLU_RS01670 0.14 +0.4 372,250 - PFLU_RS01670 0.14 -2.5 372,250 - PFLU_RS01670 0.14 +0.0 372,250 - PFLU_RS01670 0.14 -1.7 372,447 - PFLU_RS01670 0.55 -0.7 372,526 - PFLU_RS01670 0.71 +3.1 372,526 - PFLU_RS01670 0.71 -0.2 372,660 + -1.9 372,660 + -0.7 372,660 + +1.3 372,660 + +0.3 372,660 + -0.0 372,660 + -0.1 372,660 + +1.1 372,661 - +0.5 372,661 - +0.5 372,661 - -1.5 372,661 - -1.4 372,661 - -2.9 372,661 - +0.4 372,721 + +0.8 372,721 + -2.9 372,721 + -0.3 372,721 + +0.0 372,721 + +0.8 372,721 + -0.0 372,721 + +2.1 372,721 + -1.4 372,721 + -1.3 372,721 + -1.1 372,721 + +0.3 372,721 + +3.1 372,721 + -0.2 372,721 + -1.0 372,721 + -0.9 372,721 + -0.6 372,721 + -0.5 372,721 + -1.5 372,721 + -1.9 372,721 + -0.0 372,721 + +1.7 372,721 + -0.3 372,721 + +0.2 372,721 + -0.5 372,721 + +1.3 372,721 + -1.2 372,721 + -0.2 372,721 + +1.1 372,721 + +1.1 372,721 + -1.5 372,721 + -0.5 372,721 + +0.0 372,721 + -1.4 372,721 + -1.2 372,721 + -1.2 372,721 + +0.5 372,721 + -0.6 372,721 + -0.9 372,721 + +0.3 372,721 + -0.6 372,721 + +0.7 372,721 + -0.9 372,721 + -0.1 372,721 + +1.1 372,721 + +0.3 372,775 + -1.0 372,776 - -0.5 372,783 + -0.1 372,783 + +1.8 372,783 + -0.6 372,783 + +0.2 372,784 - +1.1 372,784 - +1.4 372,784 - -0.6 372,784 - +0.5 372,784 - -0.1 372,813 + -0.5 372,813 + +1.3 372,813 + -0.8 372,813 + +0.0 372,813 + -0.2 372,813 + +0.3 372,813 + -1.0 372,814 - +0.9 372,814 - +1.4 372,814 - +0.3 372,814 - -1.2 372,908 + PFLU_RS01675 0.28 +1.3 372,908 + PFLU_RS01675 0.28 -0.9 372,909 - PFLU_RS01675 0.28 -0.4 372,909 - PFLU_RS01675 0.28 +0.8 373,078 - PFLU_RS01675 0.65 +0.4 373,087 - PFLU_RS01675 0.67 +1.2 373,183 + PFLU_RS01675 0.88 -0.4 373,271 + +0.9 373,300 + PFLU_RS01680 0.15 -0.0 373,300 + PFLU_RS01680 0.15 -0.2 373,300 + PFLU_RS01680 0.15 -2.5 373,300 + PFLU_RS01680 0.15 -0.2 373,300 + PFLU_RS01680 0.15 +0.5 373,301 - PFLU_RS01680 0.15 +0.4 373,301 - PFLU_RS01680 0.15 +0.4 373,301 - PFLU_RS01680 0.15 +1.1 373,301 - PFLU_RS01680 0.15 -0.1 373,301 - PFLU_RS01680 0.15 -0.0 373,301 - PFLU_RS01680 0.15 +1.8 373,301 - PFLU_RS01680 0.15 +0.5 373,301 - PFLU_RS01680 0.15 +1.2 373,486 + PFLU_RS01680 0.57 -0.4 373,487 - PFLU_RS01680 0.57 -0.0 373,487 - PFLU_RS01680 0.57 -0.3 373,674 + -0.1 373,674 + +0.5 373,674 + -0.8 373,674 + -0.1 373,674 + -0.9 373,674 + +0.7 373,674 + -0.9 373,674 + +0.7 373,675 - -1.3 373,675 - -0.3 373,675 - -1.3 373,675 - -1.0 373,675 - +0.0 373,675 - +2.1 373,675 - +0.5 373,730 - +0.4 373,747 + -1.1 373,747 + +1.5 373,747 + -0.6 373,747 + -0.1 373,747 + +0.8 373,747 + -0.2 373,747 + +0.5 373,747 + +3.5 373,747 + +2.9 373,747 + +0.7 373,747 + -1.2 373,747 + -0.5 373,747 + -0.8 373,747 + +0.4 373,748 - -0.5 373,748 - +0.9 373,748 - -0.9 373,748 - +0.2 373,748 - -1.5 373,748 - +1.2 373,748 - +0.8 373,748 - +0.8 373,748 - +0.5 373,748 - +1.5 373,748 - -1.5 373,748 - +0.5 373,748 - +0.2 373,748 - +1.3 373,748 - +0.1 373,760 + -0.9 373,760 + -0.0 373,760 + -0.3 373,760 + -0.1 373,761 - +0.1 373,761 - +0.6 373,761 - +2.7 373,787 + +0.9 373,787 + -0.6 373,832 + -0.1 373,858 + PFLU_RS30350 0.13 -0.3 373,883 + PFLU_RS30350 0.21 +0.1 373,883 + PFLU_RS30350 0.21 -1.6 373,883 + PFLU_RS30350 0.21 +0.3 373,883 + PFLU_RS30350 0.21 -0.3 373,883 + PFLU_RS30350 0.21 -1.3 373,883 + PFLU_RS30350 0.21 +1.0 373,883 + PFLU_RS30350 0.21 +0.5 373,884 - PFLU_RS30350 0.22 -1.3 373,884 - PFLU_RS30350 0.22 -2.0 373,884 - PFLU_RS30350 0.22 +1.3 373,884 - PFLU_RS30350 0.22 +0.5 373,884 - PFLU_RS30350 0.22 +0.3 373,884 - PFLU_RS30350 0.22 +0.9 373,884 - PFLU_RS30350 0.22 +1.5 373,884 - PFLU_RS30350 0.22 +0.4 373,884 - PFLU_RS30350 0.22 +1.2 373,884 - PFLU_RS30350 0.22 -0.6 373,884 - PFLU_RS30350 0.22 +0.8 374,010 + PFLU_RS30350 0.62 -0.0 374,011 - PFLU_RS30350 0.62 -0.6 374,011 - PFLU_RS30350 0.62 -0.2 374,011 - PFLU_RS30350 0.62 +0.2 374,011 - PFLU_RS30350 0.62 -0.3 374,125 + -1.3 374,125 + -0.2 374,125 + -0.5 374,125 + -1.9 374,125 + -1.5 374,125 + +0.3 374,125 + -0.0 374,125 + +0.5 374,125 + +1.6 374,125 + -0.3 374,153 - +0.8 374,153 - -0.2 374,153 - +2.1 374,153 - +0.6 374,153 - -0.8 374,153 - +0.1 374,153 - -0.3 374,153 - -0.0 374,153 - -1.3 374,153 - -0.8 374,153 - -1.2 374,155 - -0.0 374,155 - -0.5 374,155 - -0.5 374,155 - +1.4 374,155 - +0.4 374,155 - -0.3 374,155 - -0.8 374,155 - +0.4
Or see this region's nucleotide sequence