Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22575 and HSERO_RS22580 are separated by 136 nucleotides HSERO_RS22580 and HSERO_RS22585 are separated by 13 nucleotides HSERO_RS22585 and HSERO_RS22590 are separated by 305 nucleotides
HSERO_RS22575: HSERO_RS22575 - arylesterase, at 5,177,244 to 5,178,071
_RS22575
HSERO_RS22580: HSERO_RS22580 - LysR family transcriptional regulator, at 5,178,208 to 5,178,630
_RS22580
HSERO_RS22585: HSERO_RS22585 - amidohydrolase, at 5,178,644 to 5,180,527
_RS22585
HSERO_RS22590: HSERO_RS22590 - hydrolase, at 5,180,833 to 5,181,522
_RS22590
Position (kb) 5178
5179
5180
5181 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 5177.909 kb on + strand, within HSERO_RS22575 at 5177.917 kb on - strand, within HSERO_RS22575 at 5178.066 kb on + strand at 5178.211 kb on + strand at 5178.341 kb on + strand, within HSERO_RS22580 at 5178.471 kb on - strand, within HSERO_RS22580 at 5178.510 kb on - strand, within HSERO_RS22580 at 5178.535 kb on - strand, within HSERO_RS22580 at 5178.914 kb on - strand, within HSERO_RS22585 at 5178.960 kb on - strand, within HSERO_RS22585 at 5179.043 kb on - strand, within HSERO_RS22585 at 5179.124 kb on - strand, within HSERO_RS22585 at 5179.158 kb on + strand, within HSERO_RS22585 at 5179.163 kb on + strand, within HSERO_RS22585 at 5179.163 kb on + strand, within HSERO_RS22585 at 5179.166 kb on - strand, within HSERO_RS22585 at 5179.166 kb on - strand, within HSERO_RS22585 at 5179.173 kb on + strand, within HSERO_RS22585 at 5179.173 kb on + strand, within HSERO_RS22585 at 5179.173 kb on + strand, within HSERO_RS22585 at 5179.188 kb on - strand at 5179.474 kb on + strand, within HSERO_RS22585 at 5179.707 kb on + strand, within HSERO_RS22585 at 5179.734 kb on - strand, within HSERO_RS22585 at 5179.745 kb on + strand, within HSERO_RS22585 at 5179.799 kb on + strand, within HSERO_RS22585 at 5179.847 kb on + strand, within HSERO_RS22585 at 5179.890 kb on - strand, within HSERO_RS22585 at 5179.918 kb on + strand, within HSERO_RS22585 at 5179.938 kb on - strand, within HSERO_RS22585 at 5180.028 kb on - strand, within HSERO_RS22585 at 5180.161 kb on - strand, within HSERO_RS22585 at 5180.219 kb on - strand, within HSERO_RS22585 at 5180.277 kb on - strand, within HSERO_RS22585 at 5180.469 kb on + strand at 5180.505 kb on - strand at 5180.695 kb on + strand at 5181.042 kb on - strand, within HSERO_RS22590 at 5181.052 kb on - strand, within HSERO_RS22590 at 5181.102 kb on + strand, within HSERO_RS22590 at 5181.113 kb on + strand, within HSERO_RS22590 at 5181.266 kb on + strand, within HSERO_RS22590 at 5181.277 kb on + strand, within HSERO_RS22590 at 5181.399 kb on - strand, within HSERO_RS22590 at 5181.433 kb on + strand at 5181.433 kb on + strand, within HSERO_RS22590 at 5181.459 kb on + strand at 5181.467 kb on - strand at 5181.467 kb on - strand at 5181.472 kb on - strand at 5181.472 kb on - strand at 5181.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,177,909 + HSERO_RS22575 0.80 +0.1 5,177,917 - HSERO_RS22575 0.81 -0.1 5,178,066 + +0.7 5,178,211 + -0.6 5,178,341 + HSERO_RS22580 0.31 +0.8 5,178,471 - HSERO_RS22580 0.62 -1.2 5,178,510 - HSERO_RS22580 0.71 +0.3 5,178,535 - HSERO_RS22580 0.77 +0.0 5,178,914 - HSERO_RS22585 0.14 +0.5 5,178,960 - HSERO_RS22585 0.17 -0.2 5,179,043 - HSERO_RS22585 0.21 +0.1 5,179,124 - HSERO_RS22585 0.25 -0.5 5,179,158 + HSERO_RS22585 0.27 +0.2 5,179,163 + HSERO_RS22585 0.28 -0.3 5,179,163 + HSERO_RS22585 0.28 -0.4 5,179,166 - HSERO_RS22585 0.28 +0.8 5,179,166 - HSERO_RS22585 0.28 +0.9 5,179,173 + HSERO_RS22585 0.28 +0.4 5,179,173 + HSERO_RS22585 0.28 +0.1 5,179,173 + HSERO_RS22585 0.28 +0.4 5,179,188 - +1.3 5,179,474 + HSERO_RS22585 0.44 -0.8 5,179,707 + HSERO_RS22585 0.56 -2.0 5,179,734 - HSERO_RS22585 0.58 +0.2 5,179,745 + HSERO_RS22585 0.58 +0.7 5,179,799 + HSERO_RS22585 0.61 +0.4 5,179,847 + HSERO_RS22585 0.64 -1.7 5,179,890 - HSERO_RS22585 0.66 +0.0 5,179,918 + HSERO_RS22585 0.68 +0.1 5,179,938 - HSERO_RS22585 0.69 -0.1 5,180,028 - HSERO_RS22585 0.73 -0.2 5,180,161 - HSERO_RS22585 0.81 +6.0 5,180,219 - HSERO_RS22585 0.84 +0.4 5,180,277 - HSERO_RS22585 0.87 +0.0 5,180,469 + -2.1 5,180,505 - -0.2 5,180,695 + -0.1 5,181,042 - HSERO_RS22590 0.30 -0.4 5,181,052 - HSERO_RS22590 0.32 +0.3 5,181,102 + HSERO_RS22590 0.39 +0.4 5,181,113 + HSERO_RS22590 0.41 -0.3 5,181,266 + HSERO_RS22590 0.63 +0.2 5,181,277 + HSERO_RS22590 0.64 +0.9 5,181,399 - HSERO_RS22590 0.82 -0.8 5,181,433 + +0.3 5,181,433 + HSERO_RS22590 0.87 +0.9 5,181,459 + -0.1 5,181,467 - +0.1 5,181,467 - -2.0 5,181,472 - -0.8 5,181,472 - -1.2 5,181,497 - +1.2
Or see this region's nucleotide sequence