Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04075 and HSERO_RS04080 overlap by 20 nucleotides HSERO_RS04080 and HSERO_RS04085 are separated by 228 nucleotides HSERO_RS04085 and HSERO_RS04090 are separated by 162 nucleotides HSERO_RS04090 and HSERO_RS04095 are separated by 51 nucleotides
HSERO_RS04075: HSERO_RS04075 - type IV prepilin PilV protein, at 891,356 to 892,759
_RS04075
HSERO_RS04080: HSERO_RS04080 - invasion protein, at 892,740 to 893,237
_RS04080
HSERO_RS04085: HSERO_RS04085 - twitching motility protein PilT, at 893,466 to 894,563
_RS04085
HSERO_RS04090: HSERO_RS04090 - molecular chaperone Tir, at 894,726 to 895,127
_RS04090
HSERO_RS04095: HSERO_RS04095 - hypothetical protein, at 895,179 to 896,624
_RS04095
Position (kb) 893
894
895 Strain fitness (log2 ratio) -2
-1
0
1 at 892.747 kb on + strand at 892.770 kb on - strand at 892.823 kb on + strand, within HSERO_RS04080 at 892.857 kb on + strand, within HSERO_RS04080 at 892.867 kb on + strand, within HSERO_RS04080 at 892.910 kb on + strand, within HSERO_RS04080 at 892.918 kb on - strand, within HSERO_RS04080 at 892.954 kb on - strand, within HSERO_RS04080 at 892.954 kb on - strand, within HSERO_RS04080 at 892.954 kb on - strand, within HSERO_RS04080 at 893.121 kb on + strand, within HSERO_RS04080 at 893.161 kb on + strand, within HSERO_RS04080 at 893.161 kb on + strand, within HSERO_RS04080 at 893.169 kb on - strand, within HSERO_RS04080 at 893.169 kb on - strand, within HSERO_RS04080 at 893.242 kb on - strand at 893.392 kb on + strand at 894.656 kb on - strand at 894.742 kb on + strand at 894.801 kb on + strand, within HSERO_RS04090 at 894.994 kb on + strand, within HSERO_RS04090 at 895.000 kb on - strand, within HSERO_RS04090 at 895.007 kb on - strand, within HSERO_RS04090 at 895.069 kb on + strand, within HSERO_RS04090 at 895.398 kb on + strand, within HSERO_RS04095 at 895.443 kb on + strand, within HSERO_RS04095
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 892,747 + -2.4 892,770 - +1.2 892,823 + HSERO_RS04080 0.17 +0.2 892,857 + HSERO_RS04080 0.23 +0.3 892,867 + HSERO_RS04080 0.26 +0.5 892,910 + HSERO_RS04080 0.34 -0.3 892,918 - HSERO_RS04080 0.36 -0.4 892,954 - HSERO_RS04080 0.43 -0.1 892,954 - HSERO_RS04080 0.43 +0.4 892,954 - HSERO_RS04080 0.43 +0.3 893,121 + HSERO_RS04080 0.77 +1.2 893,161 + HSERO_RS04080 0.85 +0.3 893,161 + HSERO_RS04080 0.85 +0.3 893,169 - HSERO_RS04080 0.86 -0.3 893,169 - HSERO_RS04080 0.86 -0.2 893,242 - -0.5 893,392 + -0.1 894,656 - +0.1 894,742 + +0.9 894,801 + HSERO_RS04090 0.19 +0.5 894,994 + HSERO_RS04090 0.67 +0.4 895,000 - HSERO_RS04090 0.68 -0.7 895,007 - HSERO_RS04090 0.70 -0.2 895,069 + HSERO_RS04090 0.85 +0.8 895,398 + HSERO_RS04095 0.15 +1.5 895,443 + HSERO_RS04095 0.18 -0.1
Or see this region's nucleotide sequence