Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_72

Experiment: Cobalt chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_70 and Pf6N2E2_71 are separated by 63 nucleotidesPf6N2E2_71 and Pf6N2E2_72 are separated by 252 nucleotidesPf6N2E2_72 and Pf6N2E2_73 overlap by 4 nucleotides Pf6N2E2_70 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 112,610 to 113,464 _70 Pf6N2E2_71 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 113,528 to 114,160 _71 Pf6N2E2_72 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 114,413 to 115,333 _72 Pf6N2E2_73 - Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17), at 115,330 to 116,637 _73 Position (kb) 114 115 116Strain fitness (log2 ratio) -1 0 1at 113.417 kb on + strandat 113.417 kb on + strandat 113.624 kb on + strand, within Pf6N2E2_71at 113.745 kb on + strand, within Pf6N2E2_71at 113.745 kb on + strand, within Pf6N2E2_71at 113.825 kb on + strand, within Pf6N2E2_71at 113.826 kb on - strand, within Pf6N2E2_71at 113.915 kb on + strand, within Pf6N2E2_71at 113.915 kb on + strand, within Pf6N2E2_71at 113.915 kb on + strand, within Pf6N2E2_71at 113.916 kb on - strand, within Pf6N2E2_71at 113.916 kb on - strand, within Pf6N2E2_71at 114.053 kb on + strand, within Pf6N2E2_71at 114.053 kb on + strand, within Pf6N2E2_71at 114.196 kb on + strandat 114.196 kb on + strandat 115.329 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM
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113,417 + +1.5
113,417 + -0.3
113,624 + Pf6N2E2_71 0.15 -1.7
113,745 + Pf6N2E2_71 0.34 -0.3
113,745 + Pf6N2E2_71 0.34 +0.1
113,825 + Pf6N2E2_71 0.47 -0.4
113,826 - Pf6N2E2_71 0.47 +0.0
113,915 + Pf6N2E2_71 0.61 +1.2
113,915 + Pf6N2E2_71 0.61 +0.3
113,915 + Pf6N2E2_71 0.61 -0.6
113,916 - Pf6N2E2_71 0.61 +0.8
113,916 - Pf6N2E2_71 0.61 +1.0
114,053 + Pf6N2E2_71 0.83 -0.6
114,053 + Pf6N2E2_71 0.83 +0.3
114,196 + +0.7
114,196 + -0.3
115,329 + +0.7

Or see this region's nucleotide sequence