Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_70 and Pf6N2E2_71 are separated by 63 nucleotides Pf6N2E2_71 and Pf6N2E2_72 are separated by 252 nucleotides Pf6N2E2_72 and Pf6N2E2_73 overlap by 4 nucleotides
Pf6N2E2_70 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 112,610 to 113,464
_70
Pf6N2E2_71 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 113,528 to 114,160
_71
Pf6N2E2_72 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 114,413 to 115,333
_72
Pf6N2E2_73 - Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17), at 115,330 to 116,637
_73
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-1
0
1 at 113.417 kb on + strand at 113.417 kb on + strand at 113.624 kb on + strand, within Pf6N2E2_71 at 113.745 kb on + strand, within Pf6N2E2_71 at 113.745 kb on + strand, within Pf6N2E2_71 at 113.825 kb on + strand, within Pf6N2E2_71 at 113.826 kb on - strand, within Pf6N2E2_71 at 113.915 kb on + strand, within Pf6N2E2_71 at 113.915 kb on + strand, within Pf6N2E2_71 at 113.915 kb on + strand, within Pf6N2E2_71 at 113.916 kb on - strand, within Pf6N2E2_71 at 113.916 kb on - strand, within Pf6N2E2_71 at 114.053 kb on + strand, within Pf6N2E2_71 at 114.053 kb on + strand, within Pf6N2E2_71 at 114.196 kb on + strand at 114.196 kb on + strand at 115.329 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 113,417 + +1.5 113,417 + -0.3 113,624 + Pf6N2E2_71 0.15 -1.7 113,745 + Pf6N2E2_71 0.34 -0.3 113,745 + Pf6N2E2_71 0.34 +0.1 113,825 + Pf6N2E2_71 0.47 -0.4 113,826 - Pf6N2E2_71 0.47 +0.0 113,915 + Pf6N2E2_71 0.61 +1.2 113,915 + Pf6N2E2_71 0.61 +0.3 113,915 + Pf6N2E2_71 0.61 -0.6 113,916 - Pf6N2E2_71 0.61 +0.8 113,916 - Pf6N2E2_71 0.61 +1.0 114,053 + Pf6N2E2_71 0.83 -0.6 114,053 + Pf6N2E2_71 0.83 +0.3 114,196 + +0.7 114,196 + -0.3 115,329 + +0.7
Or see this region's nucleotide sequence