Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4819 and Pf6N2E2_4820 are separated by 78 nucleotides Pf6N2E2_4820 and Pf6N2E2_4821 overlap by 4 nucleotides Pf6N2E2_4821 and Pf6N2E2_4822 are separated by 70 nucleotides
Pf6N2E2_4819 - Alcohol dehydrogenase (EC 1.1.1.1), at 5,572,498 to 5,573,646
_4819
Pf6N2E2_4820 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 5,573,725 to 5,574,399
_4820
Pf6N2E2_4821 - Phosphoglycolate phosphatase (EC 3.1.3.18), at 5,574,396 to 5,575,214
_4821
Pf6N2E2_4822 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 5,575,285 to 5,576,766
_4822
Position (kb)
5574
5575
5576 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5573.475 kb on + strand, within Pf6N2E2_4819 at 5573.475 kb on + strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.476 kb on - strand, within Pf6N2E2_4819 at 5573.499 kb on - strand, within Pf6N2E2_4819 at 5573.570 kb on + strand at 5573.630 kb on - strand at 5573.641 kb on + strand at 5573.642 kb on - strand at 5573.681 kb on + strand at 5573.681 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.683 kb on + strand at 5573.684 kb on - strand at 5573.684 kb on - strand at 5573.684 kb on - strand at 5573.684 kb on - strand at 5573.684 kb on - strand at 5574.550 kb on + strand, within Pf6N2E2_4821 at 5574.764 kb on + strand, within Pf6N2E2_4821 at 5574.764 kb on + strand, within Pf6N2E2_4821 at 5574.765 kb on - strand, within Pf6N2E2_4821 at 5574.765 kb on - strand, within Pf6N2E2_4821 at 5574.848 kb on + strand, within Pf6N2E2_4821 at 5574.999 kb on + strand, within Pf6N2E2_4821 at 5575.006 kb on - strand, within Pf6N2E2_4821 at 5575.069 kb on + strand, within Pf6N2E2_4821 at 5575.069 kb on + strand, within Pf6N2E2_4821 at 5575.069 kb on + strand, within Pf6N2E2_4821 at 5575.070 kb on - strand, within Pf6N2E2_4821 at 5575.102 kb on + strand, within Pf6N2E2_4821 at 5575.102 kb on + strand, within Pf6N2E2_4821 at 5575.102 kb on + strand, within Pf6N2E2_4821 at 5575.103 kb on - strand, within Pf6N2E2_4821 at 5575.103 kb on - strand, within Pf6N2E2_4821 at 5575.103 kb on - strand, within Pf6N2E2_4821 at 5575.103 kb on - strand, within Pf6N2E2_4821 at 5575.103 kb on - strand, within Pf6N2E2_4821 at 5575.248 kb on + strand at 5575.248 kb on + strand at 5575.249 kb on - strand at 5575.249 kb on - strand at 5575.324 kb on + strand at 5575.325 kb on - strand at 5575.385 kb on - strand at 5575.385 kb on - strand at 5575.414 kb on + strand at 5575.414 kb on + strand at 5575.414 kb on + strand at 5575.414 kb on + strand at 5575.414 kb on + strand at 5575.414 kb on + strand at 5575.414 kb on + strand at 5575.414 kb on + strand at 5575.415 kb on - strand at 5575.415 kb on - strand at 5575.456 kb on + strand, within Pf6N2E2_4822 at 5575.594 kb on + strand, within Pf6N2E2_4822 at 5575.594 kb on + strand, within Pf6N2E2_4822 at 5575.648 kb on + strand, within Pf6N2E2_4822 at 5575.673 kb on - strand, within Pf6N2E2_4822 at 5575.673 kb on - strand, within Pf6N2E2_4822 at 5575.729 kb on - strand, within Pf6N2E2_4822 at 5575.826 kb on - strand, within Pf6N2E2_4822 at 5575.826 kb on - strand, within Pf6N2E2_4822 at 5575.868 kb on + strand, within Pf6N2E2_4822 at 5575.960 kb on + strand, within Pf6N2E2_4822 at 5575.960 kb on + strand, within Pf6N2E2_4822 at 5575.990 kb on + strand, within Pf6N2E2_4822 at 5575.990 kb on + strand, within Pf6N2E2_4822 at 5575.990 kb on + strand, within Pf6N2E2_4822 at 5575.990 kb on + strand, within Pf6N2E2_4822 at 5575.990 kb on + strand, within Pf6N2E2_4822 at 5575.990 kb on + strand, within Pf6N2E2_4822 at 5575.990 kb on + strand, within Pf6N2E2_4822 at 5575.990 kb on + strand, within Pf6N2E2_4822 at 5575.990 kb on + strand, within Pf6N2E2_4822 at 5575.991 kb on - strand, within Pf6N2E2_4822 at 5575.991 kb on - strand, within Pf6N2E2_4822 at 5575.991 kb on - strand, within Pf6N2E2_4822 at 5576.018 kb on + strand, within Pf6N2E2_4822 at 5576.018 kb on + strand, within Pf6N2E2_4822 at 5576.019 kb on - strand, within Pf6N2E2_4822 at 5576.019 kb on - strand, within Pf6N2E2_4822 at 5576.104 kb on + strand, within Pf6N2E2_4822 at 5576.104 kb on + strand, within Pf6N2E2_4822 at 5576.104 kb on + strand, within Pf6N2E2_4822 at 5576.104 kb on + strand, within Pf6N2E2_4822 at 5576.104 kb on + strand, within Pf6N2E2_4822 at 5576.104 kb on + strand, within Pf6N2E2_4822 at 5576.105 kb on - strand, within Pf6N2E2_4822 at 5576.105 kb on - strand, within Pf6N2E2_4822 at 5576.105 kb on - strand, within Pf6N2E2_4822 at 5576.105 kb on - strand, within Pf6N2E2_4822 at 5576.105 kb on - strand, within Pf6N2E2_4822 at 5576.105 kb on - strand, within Pf6N2E2_4822 at 5576.105 kb on - strand, within Pf6N2E2_4822 at 5576.105 kb on - strand, within Pf6N2E2_4822 at 5576.105 kb on - strand, within Pf6N2E2_4822 at 5576.110 kb on + strand, within Pf6N2E2_4822 at 5576.110 kb on + strand, within Pf6N2E2_4822 at 5576.110 kb on + strand, within Pf6N2E2_4822 at 5576.110 kb on + strand, within Pf6N2E2_4822 at 5576.110 kb on + strand, within Pf6N2E2_4822 at 5576.110 kb on + strand, within Pf6N2E2_4822 at 5576.110 kb on + strand, within Pf6N2E2_4822 at 5576.110 kb on + strand, within Pf6N2E2_4822 at 5576.110 kb on + strand, within Pf6N2E2_4822 at 5576.111 kb on - strand, within Pf6N2E2_4822 at 5576.111 kb on - strand, within Pf6N2E2_4822 at 5576.111 kb on - strand, within Pf6N2E2_4822 at 5576.111 kb on - strand, within Pf6N2E2_4822 at 5576.111 kb on - strand, within Pf6N2E2_4822 at 5576.111 kb on - strand, within Pf6N2E2_4822 at 5576.111 kb on - strand, within Pf6N2E2_4822 at 5576.111 kb on - strand, within Pf6N2E2_4822 at 5576.111 kb on - strand, within Pf6N2E2_4822
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 5,573,475 + Pf6N2E2_4819 0.85 +1.2 5,573,475 + Pf6N2E2_4819 0.85 +0.6 5,573,476 - Pf6N2E2_4819 0.85 +0.4 5,573,476 - Pf6N2E2_4819 0.85 -0.3 5,573,476 - Pf6N2E2_4819 0.85 -1.4 5,573,476 - Pf6N2E2_4819 0.85 +0.0 5,573,476 - Pf6N2E2_4819 0.85 +1.5 5,573,499 - Pf6N2E2_4819 0.87 -0.0 5,573,570 + -1.1 5,573,630 - -1.4 5,573,641 + +0.0 5,573,642 - -0.3 5,573,681 + +1.7 5,573,681 + -0.5 5,573,683 + -1.4 5,573,683 + +1.5 5,573,683 + +0.2 5,573,683 + +0.4 5,573,683 + +0.6 5,573,683 + -0.6 5,573,683 + -0.4 5,573,683 + -0.2 5,573,683 + -0.2 5,573,684 - +1.0 5,573,684 - -0.1 5,573,684 - -0.4 5,573,684 - -0.2 5,573,684 - +0.6 5,574,550 + Pf6N2E2_4821 0.19 -0.8 5,574,764 + Pf6N2E2_4821 0.45 +0.6 5,574,764 + Pf6N2E2_4821 0.45 +0.1 5,574,765 - Pf6N2E2_4821 0.45 +1.6 5,574,765 - Pf6N2E2_4821 0.45 +3.2 5,574,848 + Pf6N2E2_4821 0.55 -0.8 5,574,999 + Pf6N2E2_4821 0.74 +2.2 5,575,006 - Pf6N2E2_4821 0.74 +0.5 5,575,069 + Pf6N2E2_4821 0.82 +0.6 5,575,069 + Pf6N2E2_4821 0.82 -0.8 5,575,069 + Pf6N2E2_4821 0.82 +0.9 5,575,070 - Pf6N2E2_4821 0.82 +1.8 5,575,102 + Pf6N2E2_4821 0.86 +1.5 5,575,102 + Pf6N2E2_4821 0.86 +1.0 5,575,102 + Pf6N2E2_4821 0.86 -0.4 5,575,103 - Pf6N2E2_4821 0.86 +1.8 5,575,103 - Pf6N2E2_4821 0.86 +1.5 5,575,103 - Pf6N2E2_4821 0.86 +0.7 5,575,103 - Pf6N2E2_4821 0.86 +0.7 5,575,103 - Pf6N2E2_4821 0.86 +0.1 5,575,248 + -0.4 5,575,248 + -0.4 5,575,249 - +2.5 5,575,249 - +0.1 5,575,324 + -0.8 5,575,325 - +0.9 5,575,385 - +0.3 5,575,385 - +0.1 5,575,414 + +0.6 5,575,414 + -0.3 5,575,414 + +0.4 5,575,414 + -0.0 5,575,414 + +1.1 5,575,414 + +0.1 5,575,414 + +0.8 5,575,414 + +0.2 5,575,415 - +0.1 5,575,415 - -0.5 5,575,456 + Pf6N2E2_4822 0.12 +1.0 5,575,594 + Pf6N2E2_4822 0.21 +0.5 5,575,594 + Pf6N2E2_4822 0.21 +1.2 5,575,648 + Pf6N2E2_4822 0.24 -2.6 5,575,673 - Pf6N2E2_4822 0.26 -1.0 5,575,673 - Pf6N2E2_4822 0.26 -0.3 5,575,729 - Pf6N2E2_4822 0.30 +0.4 5,575,826 - Pf6N2E2_4822 0.37 -0.8 5,575,826 - Pf6N2E2_4822 0.37 -0.9 5,575,868 + Pf6N2E2_4822 0.39 -0.1 5,575,960 + Pf6N2E2_4822 0.46 +0.2 5,575,960 + Pf6N2E2_4822 0.46 -0.7 5,575,990 + Pf6N2E2_4822 0.48 +1.2 5,575,990 + Pf6N2E2_4822 0.48 -1.3 5,575,990 + Pf6N2E2_4822 0.48 -0.8 5,575,990 + Pf6N2E2_4822 0.48 -1.1 5,575,990 + Pf6N2E2_4822 0.48 +0.2 5,575,990 + Pf6N2E2_4822 0.48 -0.8 5,575,990 + Pf6N2E2_4822 0.48 +1.2 5,575,990 + Pf6N2E2_4822 0.48 -0.3 5,575,990 + Pf6N2E2_4822 0.48 +0.4 5,575,991 - Pf6N2E2_4822 0.48 +0.0 5,575,991 - Pf6N2E2_4822 0.48 -0.1 5,575,991 - Pf6N2E2_4822 0.48 -1.1 5,576,018 + Pf6N2E2_4822 0.49 +0.1 5,576,018 + Pf6N2E2_4822 0.49 +0.3 5,576,019 - Pf6N2E2_4822 0.50 -0.4 5,576,019 - Pf6N2E2_4822 0.50 -2.0 5,576,104 + Pf6N2E2_4822 0.55 +0.3 5,576,104 + Pf6N2E2_4822 0.55 -0.5 5,576,104 + Pf6N2E2_4822 0.55 -0.7 5,576,104 + Pf6N2E2_4822 0.55 +2.5 5,576,104 + Pf6N2E2_4822 0.55 +0.9 5,576,104 + Pf6N2E2_4822 0.55 -0.0 5,576,105 - Pf6N2E2_4822 0.55 -0.2 5,576,105 - Pf6N2E2_4822 0.55 +0.3 5,576,105 - Pf6N2E2_4822 0.55 +1.2 5,576,105 - Pf6N2E2_4822 0.55 -0.0 5,576,105 - Pf6N2E2_4822 0.55 +0.0 5,576,105 - Pf6N2E2_4822 0.55 +0.3 5,576,105 - Pf6N2E2_4822 0.55 +0.5 5,576,105 - Pf6N2E2_4822 0.55 -0.5 5,576,105 - Pf6N2E2_4822 0.55 -0.2 5,576,110 + Pf6N2E2_4822 0.56 +0.4 5,576,110 + Pf6N2E2_4822 0.56 -2.2 5,576,110 + Pf6N2E2_4822 0.56 +0.2 5,576,110 + Pf6N2E2_4822 0.56 -0.3 5,576,110 + Pf6N2E2_4822 0.56 -0.5 5,576,110 + Pf6N2E2_4822 0.56 +0.9 5,576,110 + Pf6N2E2_4822 0.56 +0.8 5,576,110 + Pf6N2E2_4822 0.56 -1.2 5,576,110 + Pf6N2E2_4822 0.56 -0.1 5,576,111 - Pf6N2E2_4822 0.56 +0.7 5,576,111 - Pf6N2E2_4822 0.56 +0.4 5,576,111 - Pf6N2E2_4822 0.56 -0.9 5,576,111 - Pf6N2E2_4822 0.56 +0.4 5,576,111 - Pf6N2E2_4822 0.56 +1.0 5,576,111 - Pf6N2E2_4822 0.56 +0.5 5,576,111 - Pf6N2E2_4822 0.56 +2.2 5,576,111 - Pf6N2E2_4822 0.56 +1.2 5,576,111 - Pf6N2E2_4822 0.56 -0.3
Or see this region's nucleotide sequence