Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4306 and Pf6N2E2_4307 are separated by 5 nucleotides Pf6N2E2_4307 and Pf6N2E2_4308 overlap by 4 nucleotides Pf6N2E2_4308 and Pf6N2E2_4309 are separated by 5 nucleotides Pf6N2E2_4309 and Pf6N2E2_4310 are separated by 13 nucleotides
Pf6N2E2_4306 - Uncharacterized protein ImpI/VasC, at 5,022,187 to 5,023,032
_4306
Pf6N2E2_4307 - Type VI secretion lipoprotein/VasD, at 5,023,038 to 5,023,541
_4307
Pf6N2E2_4308 - Uncharacterized protein ImpJ/VasE, at 5,023,538 to 5,024,869
_4308
Pf6N2E2_4309 - Outer membrane protein ImpK/VasF, OmpA/MotB domain, at 5,024,875 to 5,025,738
_4309
Pf6N2E2_4310 - IcmF-related protein, at 5,025,752 to 5,029,195
_4310
Position (kb)
5023
5024
5025 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5022.691 kb on + strand, within Pf6N2E2_4306 at 5022.691 kb on + strand, within Pf6N2E2_4306 at 5022.699 kb on + strand, within Pf6N2E2_4306 at 5022.699 kb on + strand, within Pf6N2E2_4306 at 5022.979 kb on + strand at 5022.980 kb on - strand at 5022.980 kb on - strand at 5022.980 kb on - strand at 5022.980 kb on - strand at 5022.980 kb on - strand at 5022.980 kb on - strand at 5023.272 kb on + strand, within Pf6N2E2_4307 at 5023.365 kb on - strand, within Pf6N2E2_4307 at 5023.393 kb on + strand, within Pf6N2E2_4307 at 5023.405 kb on - strand, within Pf6N2E2_4307 at 5023.434 kb on + strand, within Pf6N2E2_4307 at 5023.528 kb on + strand at 5023.528 kb on + strand at 5023.528 kb on + strand at 5023.529 kb on - strand at 5023.529 kb on - strand at 5023.529 kb on - strand at 5023.529 kb on - strand at 5023.529 kb on - strand at 5023.610 kb on + strand at 5023.611 kb on - strand at 5023.611 kb on - strand at 5023.655 kb on + strand at 5023.655 kb on + strand at 5023.656 kb on - strand at 5023.656 kb on - strand at 5023.798 kb on - strand, within Pf6N2E2_4308 at 5023.798 kb on - strand, within Pf6N2E2_4308 at 5023.807 kb on + strand, within Pf6N2E2_4308 at 5023.807 kb on + strand, within Pf6N2E2_4308 at 5023.826 kb on + strand, within Pf6N2E2_4308 at 5023.826 kb on + strand, within Pf6N2E2_4308 at 5023.901 kb on + strand, within Pf6N2E2_4308 at 5023.902 kb on - strand, within Pf6N2E2_4308 at 5023.910 kb on + strand, within Pf6N2E2_4308 at 5023.910 kb on + strand, within Pf6N2E2_4308 at 5023.910 kb on + strand, within Pf6N2E2_4308 at 5023.910 kb on + strand, within Pf6N2E2_4308 at 5023.910 kb on + strand, within Pf6N2E2_4308 at 5023.910 kb on + strand, within Pf6N2E2_4308 at 5023.911 kb on - strand, within Pf6N2E2_4308 at 5023.911 kb on - strand, within Pf6N2E2_4308 at 5023.911 kb on - strand, within Pf6N2E2_4308 at 5024.127 kb on - strand, within Pf6N2E2_4308 at 5024.127 kb on - strand, within Pf6N2E2_4308 at 5024.127 kb on - strand, within Pf6N2E2_4308 at 5024.254 kb on + strand, within Pf6N2E2_4308 at 5024.255 kb on - strand, within Pf6N2E2_4308 at 5024.255 kb on - strand, within Pf6N2E2_4308 at 5024.286 kb on - strand, within Pf6N2E2_4308 at 5024.286 kb on - strand, within Pf6N2E2_4308 at 5024.304 kb on + strand, within Pf6N2E2_4308 at 5024.304 kb on + strand, within Pf6N2E2_4308 at 5024.304 kb on + strand, within Pf6N2E2_4308 at 5024.304 kb on + strand, within Pf6N2E2_4308 at 5024.304 kb on + strand, within Pf6N2E2_4308 at 5024.304 kb on + strand, within Pf6N2E2_4308 at 5024.304 kb on + strand, within Pf6N2E2_4308 at 5024.305 kb on - strand, within Pf6N2E2_4308 at 5024.305 kb on - strand, within Pf6N2E2_4308 at 5024.305 kb on - strand, within Pf6N2E2_4308 at 5024.320 kb on - strand, within Pf6N2E2_4308 at 5024.322 kb on - strand, within Pf6N2E2_4308 at 5024.331 kb on + strand, within Pf6N2E2_4308 at 5024.332 kb on - strand, within Pf6N2E2_4308 at 5024.408 kb on - strand, within Pf6N2E2_4308 at 5024.408 kb on - strand, within Pf6N2E2_4308 at 5024.747 kb on + strand at 5024.747 kb on + strand at 5024.747 kb on + strand at 5024.747 kb on + strand at 5024.748 kb on - strand at 5024.788 kb on + strand at 5024.987 kb on + strand, within Pf6N2E2_4309 at 5024.987 kb on + strand, within Pf6N2E2_4309 at 5024.988 kb on - strand, within Pf6N2E2_4309 at 5025.004 kb on + strand, within Pf6N2E2_4309 at 5025.223 kb on + strand, within Pf6N2E2_4309 at 5025.224 kb on - strand, within Pf6N2E2_4309 at 5025.296 kb on + strand, within Pf6N2E2_4309 at 5025.297 kb on - strand, within Pf6N2E2_4309 at 5025.297 kb on - strand, within Pf6N2E2_4309 at 5025.297 kb on - strand, within Pf6N2E2_4309 at 5025.409 kb on + strand, within Pf6N2E2_4309 at 5025.409 kb on + strand, within Pf6N2E2_4309 at 5025.409 kb on + strand, within Pf6N2E2_4309 at 5025.409 kb on + strand, within Pf6N2E2_4309 at 5025.410 kb on - strand, within Pf6N2E2_4309 at 5025.410 kb on - strand, within Pf6N2E2_4309 at 5025.410 kb on - strand, within Pf6N2E2_4309 at 5025.497 kb on - strand, within Pf6N2E2_4309 at 5025.559 kb on + strand, within Pf6N2E2_4309 at 5025.560 kb on - strand, within Pf6N2E2_4309 at 5025.643 kb on + strand, within Pf6N2E2_4309 at 5025.697 kb on + strand at 5025.736 kb on + strand at 5025.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 5,022,691 + Pf6N2E2_4306 0.60 -0.2 5,022,691 + Pf6N2E2_4306 0.60 +1.8 5,022,699 + Pf6N2E2_4306 0.61 +0.8 5,022,699 + Pf6N2E2_4306 0.61 +0.7 5,022,979 + -0.3 5,022,980 - +1.2 5,022,980 - +0.1 5,022,980 - -1.4 5,022,980 - +0.1 5,022,980 - -0.9 5,022,980 - -0.8 5,023,272 + Pf6N2E2_4307 0.46 +0.8 5,023,365 - Pf6N2E2_4307 0.65 +0.2 5,023,393 + Pf6N2E2_4307 0.70 -1.7 5,023,405 - Pf6N2E2_4307 0.73 -0.7 5,023,434 + Pf6N2E2_4307 0.79 -1.2 5,023,528 + -0.3 5,023,528 + +1.2 5,023,528 + +0.4 5,023,529 - -0.1 5,023,529 - -0.8 5,023,529 - -0.4 5,023,529 - +0.6 5,023,529 - -0.1 5,023,610 + +0.7 5,023,611 - -0.3 5,023,611 - -0.1 5,023,655 + +0.1 5,023,655 + -0.3 5,023,656 - -0.9 5,023,656 - +0.1 5,023,798 - Pf6N2E2_4308 0.20 -0.4 5,023,798 - Pf6N2E2_4308 0.20 -0.4 5,023,807 + Pf6N2E2_4308 0.20 -0.5 5,023,807 + Pf6N2E2_4308 0.20 -1.6 5,023,826 + Pf6N2E2_4308 0.22 -0.3 5,023,826 + Pf6N2E2_4308 0.22 +1.0 5,023,901 + Pf6N2E2_4308 0.27 -1.2 5,023,902 - Pf6N2E2_4308 0.27 -2.3 5,023,910 + Pf6N2E2_4308 0.28 -1.1 5,023,910 + Pf6N2E2_4308 0.28 -1.6 5,023,910 + Pf6N2E2_4308 0.28 +0.4 5,023,910 + Pf6N2E2_4308 0.28 -0.4 5,023,910 + Pf6N2E2_4308 0.28 -0.4 5,023,910 + Pf6N2E2_4308 0.28 -1.0 5,023,911 - Pf6N2E2_4308 0.28 -0.6 5,023,911 - Pf6N2E2_4308 0.28 -1.2 5,023,911 - Pf6N2E2_4308 0.28 -1.8 5,024,127 - Pf6N2E2_4308 0.44 -0.1 5,024,127 - Pf6N2E2_4308 0.44 +0.1 5,024,127 - Pf6N2E2_4308 0.44 +1.4 5,024,254 + Pf6N2E2_4308 0.54 -2.1 5,024,255 - Pf6N2E2_4308 0.54 +0.4 5,024,255 - Pf6N2E2_4308 0.54 -0.5 5,024,286 - Pf6N2E2_4308 0.56 +1.4 5,024,286 - Pf6N2E2_4308 0.56 +0.2 5,024,304 + Pf6N2E2_4308 0.58 -0.9 5,024,304 + Pf6N2E2_4308 0.58 -0.2 5,024,304 + Pf6N2E2_4308 0.58 +0.0 5,024,304 + Pf6N2E2_4308 0.58 +0.2 5,024,304 + Pf6N2E2_4308 0.58 +0.1 5,024,304 + Pf6N2E2_4308 0.58 +1.1 5,024,304 + Pf6N2E2_4308 0.58 -0.7 5,024,305 - Pf6N2E2_4308 0.58 -1.3 5,024,305 - Pf6N2E2_4308 0.58 +0.3 5,024,305 - Pf6N2E2_4308 0.58 +0.6 5,024,320 - Pf6N2E2_4308 0.59 -0.2 5,024,322 - Pf6N2E2_4308 0.59 -0.3 5,024,331 + Pf6N2E2_4308 0.60 +1.4 5,024,332 - Pf6N2E2_4308 0.60 -0.5 5,024,408 - Pf6N2E2_4308 0.65 +0.5 5,024,408 - Pf6N2E2_4308 0.65 +0.6 5,024,747 + +0.8 5,024,747 + +0.6 5,024,747 + +2.4 5,024,747 + -0.0 5,024,748 - -0.4 5,024,788 + +1.5 5,024,987 + Pf6N2E2_4309 0.13 +0.5 5,024,987 + Pf6N2E2_4309 0.13 +1.1 5,024,988 - Pf6N2E2_4309 0.13 +1.4 5,025,004 + Pf6N2E2_4309 0.15 -0.7 5,025,223 + Pf6N2E2_4309 0.40 -0.4 5,025,224 - Pf6N2E2_4309 0.40 +1.1 5,025,296 + Pf6N2E2_4309 0.49 +2.4 5,025,297 - Pf6N2E2_4309 0.49 -1.2 5,025,297 - Pf6N2E2_4309 0.49 +1.1 5,025,297 - Pf6N2E2_4309 0.49 -1.2 5,025,409 + Pf6N2E2_4309 0.62 -0.1 5,025,409 + Pf6N2E2_4309 0.62 +3.7 5,025,409 + Pf6N2E2_4309 0.62 -0.7 5,025,409 + Pf6N2E2_4309 0.62 -0.0 5,025,410 - Pf6N2E2_4309 0.62 +0.6 5,025,410 - Pf6N2E2_4309 0.62 -0.4 5,025,410 - Pf6N2E2_4309 0.62 -1.2 5,025,497 - Pf6N2E2_4309 0.72 +0.4 5,025,559 + Pf6N2E2_4309 0.79 -0.1 5,025,560 - Pf6N2E2_4309 0.79 -0.7 5,025,643 + Pf6N2E2_4309 0.89 +0.6 5,025,697 + -0.0 5,025,736 + +0.7 5,025,737 - +0.9
Or see this region's nucleotide sequence