Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_358

Experiment: Cobalt chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_356 and Pf6N2E2_357 are separated by 156 nucleotidesPf6N2E2_357 and Pf6N2E2_358 are separated by 21 nucleotidesPf6N2E2_358 and Pf6N2E2_359 are separated by 36 nucleotides Pf6N2E2_356 - hypothetical protein, at 421,875 to 422,822 _356 Pf6N2E2_357 - hypothetical protein, at 422,979 to 423,353 _357 Pf6N2E2_358 - Haloacid dehalogenase, type II, at 423,375 to 424,043 _358 Pf6N2E2_359 - Opine oxidase subunit B, at 424,080 to 425,354 _359 Position (kb) 423 424 425Strain fitness (log2 ratio) -2 -1 0 1 2 3at 423.018 kb on + strand, within Pf6N2E2_357at 423.023 kb on + strand, within Pf6N2E2_357at 423.023 kb on + strand, within Pf6N2E2_357at 423.023 kb on + strand, within Pf6N2E2_357at 423.137 kb on + strand, within Pf6N2E2_357at 423.137 kb on + strand, within Pf6N2E2_357at 423.138 kb on - strand, within Pf6N2E2_357at 423.138 kb on - strand, within Pf6N2E2_357at 423.282 kb on + strand, within Pf6N2E2_357at 423.282 kb on + strand, within Pf6N2E2_357at 423.283 kb on - strand, within Pf6N2E2_357at 423.283 kb on - strand, within Pf6N2E2_357at 423.396 kb on + strandat 423.396 kb on + strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.556 kb on - strand, within Pf6N2E2_358at 423.633 kb on + strand, within Pf6N2E2_358at 423.633 kb on + strand, within Pf6N2E2_358at 423.781 kb on - strand, within Pf6N2E2_358at 423.813 kb on + strand, within Pf6N2E2_358at 424.095 kb on + strandat 424.360 kb on - strand, within Pf6N2E2_359at 424.363 kb on - strand, within Pf6N2E2_359at 424.371 kb on + strand, within Pf6N2E2_359at 424.477 kb on - strandat 424.861 kb on + strand, within Pf6N2E2_359at 424.862 kb on - strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.935 kb on + strand, within Pf6N2E2_359at 424.936 kb on - strand, within Pf6N2E2_359

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM
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423,018 + Pf6N2E2_357 0.10 +0.3
423,023 + Pf6N2E2_357 0.12 +0.9
423,023 + Pf6N2E2_357 0.12 +0.6
423,023 + Pf6N2E2_357 0.12 +1.6
423,137 + Pf6N2E2_357 0.42 +0.6
423,137 + Pf6N2E2_357 0.42 -0.3
423,138 - Pf6N2E2_357 0.42 +0.2
423,138 - Pf6N2E2_357 0.42 +2.8
423,282 + Pf6N2E2_357 0.81 +0.3
423,282 + Pf6N2E2_357 0.81 +1.6
423,283 - Pf6N2E2_357 0.81 +0.3
423,283 - Pf6N2E2_357 0.81 +0.3
423,396 + +0.6
423,396 + -0.1
423,397 - -0.7
423,397 - -0.1
423,397 - -0.4
423,397 - +0.2
423,397 - +0.2
423,397 - -1.0
423,556 - Pf6N2E2_358 0.27 -2.0
423,633 + Pf6N2E2_358 0.39 -0.6
423,633 + Pf6N2E2_358 0.39 -0.5
423,781 - Pf6N2E2_358 0.61 -0.9
423,813 + Pf6N2E2_358 0.65 -1.2
424,095 + -1.2
424,360 - Pf6N2E2_359 0.22 +0.6
424,363 - Pf6N2E2_359 0.22 -0.4
424,371 + Pf6N2E2_359 0.23 +1.6
424,477 - +1.6
424,861 + Pf6N2E2_359 0.61 -0.4
424,862 - Pf6N2E2_359 0.61 -0.8
424,908 + Pf6N2E2_359 0.65 -0.3
424,908 + Pf6N2E2_359 0.65 +0.2
424,908 + Pf6N2E2_359 0.65 -0.4
424,908 + Pf6N2E2_359 0.65 -0.4
424,908 + Pf6N2E2_359 0.65 +0.0
424,908 + Pf6N2E2_359 0.65 -0.4
424,908 + Pf6N2E2_359 0.65 -1.3
424,908 + Pf6N2E2_359 0.65 +0.6
424,908 + Pf6N2E2_359 0.65 -0.1
424,909 - Pf6N2E2_359 0.65 -0.1
424,909 - Pf6N2E2_359 0.65 -0.1
424,909 - Pf6N2E2_359 0.65 +0.9
424,909 - Pf6N2E2_359 0.65 -0.1
424,909 - Pf6N2E2_359 0.65 +1.9
424,909 - Pf6N2E2_359 0.65 +0.2
424,909 - Pf6N2E2_359 0.65 +0.1
424,935 + Pf6N2E2_359 0.67 -0.1
424,936 - Pf6N2E2_359 0.67 -0.2

Or see this region's nucleotide sequence