Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2699 and Pf6N2E2_2700 are separated by 431 nucleotides Pf6N2E2_2700 and Pf6N2E2_2701 are separated by 147 nucleotides
Pf6N2E2_2699 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO, at 3,192,596 to 3,193,534
_2699
Pf6N2E2_2700 - Cysteine synthase (EC 2.5.1.47), at 3,193,966 to 3,194,940
_2700
Pf6N2E2_2701 - FIG00954689: hypothetical protein, at 3,195,088 to 3,196,155
_2701
Position (kb)
3193
3194
3195 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3193.189 kb on - strand, within Pf6N2E2_2699 at 3193.261 kb on - strand, within Pf6N2E2_2699 at 3193.261 kb on - strand, within Pf6N2E2_2699 at 3193.261 kb on - strand, within Pf6N2E2_2699 at 3193.353 kb on + strand, within Pf6N2E2_2699 at 3193.353 kb on + strand, within Pf6N2E2_2699 at 3193.353 kb on + strand, within Pf6N2E2_2699 at 3193.383 kb on + strand, within Pf6N2E2_2699 at 3193.384 kb on - strand, within Pf6N2E2_2699 at 3193.396 kb on + strand, within Pf6N2E2_2699 at 3193.397 kb on - strand, within Pf6N2E2_2699 at 3193.399 kb on - strand, within Pf6N2E2_2699 at 3193.399 kb on - strand, within Pf6N2E2_2699 at 3193.399 kb on - strand, within Pf6N2E2_2699 at 3193.473 kb on + strand at 3193.473 kb on + strand at 3193.575 kb on - strand at 3193.586 kb on + strand at 3193.675 kb on + strand at 3193.675 kb on + strand at 3193.725 kb on + strand at 3193.725 kb on + strand at 3193.726 kb on - strand at 3193.726 kb on - strand at 3193.726 kb on - strand at 3193.726 kb on - strand at 3193.889 kb on + strand at 3193.889 kb on + strand at 3193.890 kb on - strand at 3193.890 kb on - strand at 3194.021 kb on + strand at 3194.021 kb on + strand at 3194.022 kb on - strand at 3194.189 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.523 kb on - strand, within Pf6N2E2_2700 at 3194.523 kb on - strand, within Pf6N2E2_2700 at 3194.678 kb on + strand, within Pf6N2E2_2700 at 3194.678 kb on + strand, within Pf6N2E2_2700 at 3194.679 kb on - strand, within Pf6N2E2_2700 at 3194.679 kb on - strand, within Pf6N2E2_2700 at 3194.898 kb on + strand at 3194.898 kb on + strand at 3194.899 kb on - strand at 3194.899 kb on - strand at 3194.899 kb on - strand at 3194.899 kb on - strand at 3194.929 kb on + strand at 3194.930 kb on - strand at 3194.931 kb on + strand at 3194.931 kb on + strand at 3194.931 kb on + strand at 3194.931 kb on + strand at 3194.931 kb on + strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.968 kb on + strand at 3194.969 kb on - strand at 3194.982 kb on + strand at 3194.982 kb on + strand at 3194.983 kb on - strand at 3195.048 kb on + strand at 3195.048 kb on + strand at 3195.048 kb on + strand at 3195.049 kb on - strand at 3195.049 kb on - strand at 3195.049 kb on - strand at 3195.169 kb on + strand at 3195.170 kb on - strand at 3195.370 kb on + strand, within Pf6N2E2_2701 at 3195.610 kb on + strand, within Pf6N2E2_2701 at 3195.611 kb on - strand, within Pf6N2E2_2701 at 3195.611 kb on - strand, within Pf6N2E2_2701 at 3195.802 kb on + strand, within Pf6N2E2_2701 at 3195.880 kb on + strand, within Pf6N2E2_2701 at 3195.880 kb on + strand, within Pf6N2E2_2701 at 3195.881 kb on - strand, within Pf6N2E2_2701
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 3,193,189 - Pf6N2E2_2699 0.63 +1.1 3,193,261 - Pf6N2E2_2699 0.71 +0.5 3,193,261 - Pf6N2E2_2699 0.71 -0.9 3,193,261 - Pf6N2E2_2699 0.71 -1.3 3,193,353 + Pf6N2E2_2699 0.81 +0.9 3,193,353 + Pf6N2E2_2699 0.81 +0.0 3,193,353 + Pf6N2E2_2699 0.81 -0.1 3,193,383 + Pf6N2E2_2699 0.84 +1.3 3,193,384 - Pf6N2E2_2699 0.84 +0.3 3,193,396 + Pf6N2E2_2699 0.85 +0.1 3,193,397 - Pf6N2E2_2699 0.85 +0.5 3,193,399 - Pf6N2E2_2699 0.86 -0.4 3,193,399 - Pf6N2E2_2699 0.86 -0.8 3,193,399 - Pf6N2E2_2699 0.86 -0.1 3,193,473 + -0.1 3,193,473 + +0.2 3,193,575 - +0.1 3,193,586 + -1.1 3,193,675 + +0.2 3,193,675 + +1.0 3,193,725 + -0.7 3,193,725 + +0.6 3,193,726 - +0.8 3,193,726 - -1.4 3,193,726 - -0.1 3,193,726 - +0.2 3,193,889 + -0.7 3,193,889 + -1.3 3,193,890 - -0.8 3,193,890 - -0.6 3,194,021 + -0.8 3,194,021 + -1.6 3,194,022 - -0.8 3,194,189 - Pf6N2E2_2700 0.23 -0.6 3,194,373 - Pf6N2E2_2700 0.42 -0.3 3,194,373 - Pf6N2E2_2700 0.42 -1.3 3,194,373 - Pf6N2E2_2700 0.42 +0.1 3,194,373 - Pf6N2E2_2700 0.42 -0.9 3,194,522 + Pf6N2E2_2700 0.57 -1.8 3,194,522 + Pf6N2E2_2700 0.57 -0.6 3,194,522 + Pf6N2E2_2700 0.57 -1.4 3,194,522 + Pf6N2E2_2700 0.57 -1.5 3,194,522 + Pf6N2E2_2700 0.57 -0.6 3,194,523 - Pf6N2E2_2700 0.57 -1.1 3,194,523 - Pf6N2E2_2700 0.57 -1.9 3,194,678 + Pf6N2E2_2700 0.73 +1.9 3,194,678 + Pf6N2E2_2700 0.73 +0.5 3,194,679 - Pf6N2E2_2700 0.73 -1.3 3,194,679 - Pf6N2E2_2700 0.73 -0.5 3,194,898 + -0.3 3,194,898 + -0.8 3,194,899 - -1.1 3,194,899 - -0.7 3,194,899 - -0.7 3,194,899 - -1.4 3,194,929 + -2.4 3,194,930 - -0.4 3,194,931 + -1.3 3,194,931 + -1.0 3,194,931 + -0.4 3,194,931 + -0.1 3,194,931 + -0.2 3,194,932 - -0.1 3,194,932 - -1.9 3,194,932 - +0.4 3,194,932 - -0.7 3,194,932 - +0.0 3,194,932 - -0.3 3,194,932 - -2.6 3,194,968 + -0.8 3,194,969 - -0.7 3,194,982 + -2.7 3,194,982 + +0.2 3,194,983 - -0.4 3,195,048 + -0.3 3,195,048 + -1.9 3,195,048 + -1.0 3,195,049 - +0.9 3,195,049 - +0.2 3,195,049 - +1.4 3,195,169 + +0.5 3,195,170 - +2.0 3,195,370 + Pf6N2E2_2701 0.26 +0.3 3,195,610 + Pf6N2E2_2701 0.49 -0.7 3,195,611 - Pf6N2E2_2701 0.49 +0.9 3,195,611 - Pf6N2E2_2701 0.49 -0.3 3,195,802 + Pf6N2E2_2701 0.67 +0.6 3,195,880 + Pf6N2E2_2701 0.74 +1.2 3,195,880 + Pf6N2E2_2701 0.74 -1.3 3,195,881 - Pf6N2E2_2701 0.74 +0.0
Or see this region's nucleotide sequence