Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6029 and Pf6N2E2_6030 overlap by 10 nucleotides Pf6N2E2_6030 and Pf6N2E2_6031 are separated by 185 nucleotides Pf6N2E2_6031 and Pf6N2E2_6032 are separated by 161 nucleotides Pf6N2E2_6032 and Pf6N2E2_6033 are separated by 88 nucleotides
Pf6N2E2_6029 - hypothetical protein, at 6,846,836 to 6,847,201
_6029
Pf6N2E2_6030 - Diaminopimelate epimerase (EC 5.1.1.7), at 6,847,192 to 6,847,995
_6030
Pf6N2E2_6031 - hypothetical protein, at 6,848,181 to 6,848,705
_6031
Pf6N2E2_6032 - acetyltransferase, GNAT family, at 6,848,867 to 6,849,340
_6032
Pf6N2E2_6033 - Sterol desaturase, at 6,849,429 to 6,850,526
_6033
Position (kb)
6848
6849 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6847.510 kb on - strand, within Pf6N2E2_6030 at 6847.654 kb on + strand, within Pf6N2E2_6030 at 6847.654 kb on + strand, within Pf6N2E2_6030 at 6847.654 kb on + strand, within Pf6N2E2_6030 at 6847.655 kb on - strand, within Pf6N2E2_6030 at 6847.773 kb on + strand, within Pf6N2E2_6030 at 6847.774 kb on - strand, within Pf6N2E2_6030 at 6848.083 kb on + strand at 6848.192 kb on + strand at 6848.193 kb on - strand at 6848.274 kb on - strand, within Pf6N2E2_6031 at 6848.317 kb on + strand, within Pf6N2E2_6031 at 6848.318 kb on - strand, within Pf6N2E2_6031 at 6848.318 kb on - strand, within Pf6N2E2_6031 at 6848.318 kb on - strand, within Pf6N2E2_6031 at 6848.369 kb on - strand, within Pf6N2E2_6031 at 6848.369 kb on - strand, within Pf6N2E2_6031 at 6848.551 kb on + strand, within Pf6N2E2_6031 at 6848.551 kb on + strand, within Pf6N2E2_6031 at 6848.741 kb on + strand at 6848.741 kb on + strand at 6848.742 kb on - strand at 6848.742 kb on - strand at 6848.820 kb on + strand at 6848.820 kb on + strand at 6848.821 kb on - strand at 6848.821 kb on - strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.824 kb on + strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.825 kb on - strand at 6848.847 kb on + strand at 6848.847 kb on + strand at 6848.847 kb on + strand at 6848.893 kb on + strand at 6848.893 kb on + strand at 6848.926 kb on + strand, within Pf6N2E2_6032 at 6848.926 kb on + strand, within Pf6N2E2_6032 at 6848.926 kb on + strand, within Pf6N2E2_6032 at 6848.927 kb on - strand, within Pf6N2E2_6032 at 6848.927 kb on - strand, within Pf6N2E2_6032 at 6848.927 kb on - strand, within Pf6N2E2_6032 at 6849.037 kb on + strand, within Pf6N2E2_6032 at 6849.038 kb on - strand, within Pf6N2E2_6032 at 6849.038 kb on - strand, within Pf6N2E2_6032 at 6849.187 kb on - strand, within Pf6N2E2_6032 at 6849.278 kb on - strand, within Pf6N2E2_6032 at 6849.278 kb on - strand, within Pf6N2E2_6032 at 6849.283 kb on + strand, within Pf6N2E2_6032 at 6849.283 kb on + strand, within Pf6N2E2_6032 at 6849.313 kb on + strand at 6849.313 kb on + strand at 6849.313 kb on + strand at 6849.394 kb on + strand at 6849.450 kb on + strand at 6849.450 kb on + strand at 6849.451 kb on - strand at 6849.451 kb on - strand at 6849.451 kb on - strand at 6849.451 kb on - strand at 6849.552 kb on + strand, within Pf6N2E2_6033 at 6849.553 kb on - strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.681 kb on + strand, within Pf6N2E2_6033 at 6849.681 kb on + strand, within Pf6N2E2_6033
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 6,847,510 - Pf6N2E2_6030 0.40 -1.2 6,847,654 + Pf6N2E2_6030 0.57 -0.1 6,847,654 + Pf6N2E2_6030 0.57 +0.5 6,847,654 + Pf6N2E2_6030 0.57 -0.1 6,847,655 - Pf6N2E2_6030 0.58 +1.1 6,847,773 + Pf6N2E2_6030 0.72 -0.2 6,847,774 - Pf6N2E2_6030 0.72 +3.1 6,848,083 + -0.7 6,848,192 + +1.1 6,848,193 - +1.4 6,848,274 - Pf6N2E2_6031 0.18 +0.1 6,848,317 + Pf6N2E2_6031 0.26 -0.5 6,848,318 - Pf6N2E2_6031 0.26 -0.7 6,848,318 - Pf6N2E2_6031 0.26 -0.5 6,848,318 - Pf6N2E2_6031 0.26 +0.8 6,848,369 - Pf6N2E2_6031 0.36 +0.5 6,848,369 - Pf6N2E2_6031 0.36 -0.5 6,848,551 + Pf6N2E2_6031 0.70 +0.1 6,848,551 + Pf6N2E2_6031 0.70 +0.1 6,848,741 + +1.5 6,848,741 + -1.0 6,848,742 - +1.1 6,848,742 - +0.1 6,848,820 + -0.9 6,848,820 + -1.8 6,848,821 - +1.1 6,848,821 - +0.5 6,848,824 + -1.2 6,848,824 + +0.9 6,848,824 + -0.9 6,848,824 + -1.1 6,848,824 + -0.9 6,848,824 + +0.3 6,848,824 + -1.7 6,848,824 + -0.5 6,848,824 + +0.8 6,848,824 + -0.4 6,848,825 - +0.8 6,848,825 - +0.9 6,848,825 - -0.5 6,848,825 - -0.3 6,848,825 - -0.5 6,848,825 - +1.1 6,848,825 - +0.3 6,848,825 - -1.2 6,848,825 - -0.5 6,848,825 - +0.4 6,848,825 - +1.3 6,848,847 + +0.3 6,848,847 + +0.1 6,848,847 + -0.5 6,848,893 + -1.7 6,848,893 + -0.2 6,848,926 + Pf6N2E2_6032 0.12 +0.1 6,848,926 + Pf6N2E2_6032 0.12 +0.2 6,848,926 + Pf6N2E2_6032 0.12 +0.1 6,848,927 - Pf6N2E2_6032 0.13 +1.8 6,848,927 - Pf6N2E2_6032 0.13 +0.1 6,848,927 - Pf6N2E2_6032 0.13 +0.1 6,849,037 + Pf6N2E2_6032 0.36 -0.3 6,849,038 - Pf6N2E2_6032 0.36 +0.5 6,849,038 - Pf6N2E2_6032 0.36 -0.4 6,849,187 - Pf6N2E2_6032 0.68 -0.8 6,849,278 - Pf6N2E2_6032 0.87 +0.5 6,849,278 - Pf6N2E2_6032 0.87 -1.5 6,849,283 + Pf6N2E2_6032 0.88 -0.5 6,849,283 + Pf6N2E2_6032 0.88 +0.1 6,849,313 + +2.1 6,849,313 + +1.5 6,849,313 + -0.5 6,849,394 + +0.1 6,849,450 + -0.4 6,849,450 + +2.1 6,849,451 - +1.1 6,849,451 - -1.5 6,849,451 - +0.1 6,849,451 - -0.1 6,849,552 + Pf6N2E2_6033 0.11 -0.9 6,849,553 - Pf6N2E2_6033 0.11 +1.1 6,849,678 + Pf6N2E2_6033 0.23 +0.5 6,849,678 + Pf6N2E2_6033 0.23 -0.3 6,849,678 + Pf6N2E2_6033 0.23 -1.1 6,849,678 + Pf6N2E2_6033 0.23 +0.3 6,849,681 + Pf6N2E2_6033 0.23 +0.4 6,849,681 + Pf6N2E2_6033 0.23 -0.1
Or see this region's nucleotide sequence