Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5063

Experiment: Cobalt chloride 0.4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5061 and Pf6N2E2_5062 overlap by 4 nucleotidesPf6N2E2_5062 and Pf6N2E2_5063 are separated by 286 nucleotidesPf6N2E2_5063 and Pf6N2E2_5064 overlap by 4 nucleotides Pf6N2E2_5061 - Quinate/shikimate 5-dehydrogenase I delta (EC 1.1.1.25), at 5,836,431 to 5,837,288 _5061 Pf6N2E2_5062 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 5,837,285 to 5,837,728 _5062 Pf6N2E2_5063 - tRNA (Uracil54-C5-)-methyltransferase (EC 2.1.1.35), at 5,838,015 to 5,839,094 _5063 Pf6N2E2_5064 - Xanthine/uracil/thiamine/ascorbate permease family protein, at 5,839,091 to 5,840,386 _5064 Position (kb) 5838 5839 5840Strain fitness (log2 ratio) -1 0 1 2at 5837.180 kb on - strand, within Pf6N2E2_5061at 5837.180 kb on - strand, within Pf6N2E2_5061at 5837.180 kb on - strand, within Pf6N2E2_5061at 5837.273 kb on + strandat 5837.273 kb on + strandat 5837.288 kb on + strandat 5837.289 kb on - strandat 5837.444 kb on + strand, within Pf6N2E2_5062at 5837.445 kb on - strand, within Pf6N2E2_5062at 5837.658 kb on + strand, within Pf6N2E2_5062at 5837.658 kb on + strand, within Pf6N2E2_5062at 5837.658 kb on + strand, within Pf6N2E2_5062at 5837.659 kb on - strand, within Pf6N2E2_5062at 5837.659 kb on - strand, within Pf6N2E2_5062at 5837.795 kb on - strandat 5837.802 kb on + strandat 5837.815 kb on + strandat 5837.815 kb on + strandat 5837.816 kb on - strandat 5837.816 kb on - strandat 5837.837 kb on + strandat 5837.837 kb on + strandat 5837.837 kb on + strandat 5837.837 kb on + strandat 5837.837 kb on + strandat 5837.838 kb on - strandat 5837.838 kb on - strandat 5838.016 kb on + strandat 5838.047 kb on + strandat 5838.047 kb on + strandat 5838.048 kb on - strandat 5838.048 kb on - strandat 5838.048 kb on - strandat 5838.048 kb on - strandat 5838.089 kb on + strandat 5838.090 kb on - strandat 5838.154 kb on + strand, within Pf6N2E2_5063at 5838.154 kb on + strand, within Pf6N2E2_5063at 5838.154 kb on + strand, within Pf6N2E2_5063at 5838.154 kb on + strand, within Pf6N2E2_5063at 5838.155 kb on - strand, within Pf6N2E2_5063at 5838.155 kb on - strand, within Pf6N2E2_5063at 5838.155 kb on - strand, within Pf6N2E2_5063at 5838.155 kb on - strand, within Pf6N2E2_5063at 5838.464 kb on - strand, within Pf6N2E2_5063at 5838.464 kb on - strand, within Pf6N2E2_5063at 5838.464 kb on - strand, within Pf6N2E2_5063at 5838.506 kb on - strand, within Pf6N2E2_5063at 5838.584 kb on + strand, within Pf6N2E2_5063at 5838.584 kb on + strand, within Pf6N2E2_5063at 5838.585 kb on - strand, within Pf6N2E2_5063at 5838.616 kb on + strand, within Pf6N2E2_5063at 5838.616 kb on + strand, within Pf6N2E2_5063at 5838.616 kb on + strand, within Pf6N2E2_5063at 5838.616 kb on + strand, within Pf6N2E2_5063at 5838.617 kb on - strand, within Pf6N2E2_5063at 5838.617 kb on - strand, within Pf6N2E2_5063at 5838.617 kb on - strand, within Pf6N2E2_5063at 5838.617 kb on - strand, within Pf6N2E2_5063at 5838.617 kb on - strand, within Pf6N2E2_5063at 5838.617 kb on - strand, within Pf6N2E2_5063at 5838.617 kb on - strand, within Pf6N2E2_5063at 5838.730 kb on + strand, within Pf6N2E2_5063at 5838.730 kb on + strand, within Pf6N2E2_5063at 5838.730 kb on + strand, within Pf6N2E2_5063at 5838.731 kb on - strand, within Pf6N2E2_5063at 5838.923 kb on - strand, within Pf6N2E2_5063at 5839.072 kb on + strandat 5839.072 kb on + strandat 5839.072 kb on + strandat 5839.233 kb on + strand, within Pf6N2E2_5064at 5839.233 kb on + strand, within Pf6N2E2_5064at 5839.234 kb on - strand, within Pf6N2E2_5064at 5839.479 kb on + strand, within Pf6N2E2_5064at 5839.479 kb on + strand, within Pf6N2E2_5064at 5839.479 kb on + strand, within Pf6N2E2_5064at 5839.479 kb on + strand, within Pf6N2E2_5064at 5839.479 kb on + strand, within Pf6N2E2_5064at 5839.480 kb on - strand, within Pf6N2E2_5064at 5839.665 kb on - strand, within Pf6N2E2_5064

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM
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5,837,180 - Pf6N2E2_5061 0.87 +0.0
5,837,180 - Pf6N2E2_5061 0.87 +0.4
5,837,180 - Pf6N2E2_5061 0.87 +1.1
5,837,273 + -1.0
5,837,273 + +0.2
5,837,288 + +0.4
5,837,289 - +0.2
5,837,444 + Pf6N2E2_5062 0.36 +0.1
5,837,445 - Pf6N2E2_5062 0.36 +0.8
5,837,658 + Pf6N2E2_5062 0.84 +0.5
5,837,658 + Pf6N2E2_5062 0.84 -0.6
5,837,658 + Pf6N2E2_5062 0.84 -1.5
5,837,659 - Pf6N2E2_5062 0.84 -0.8
5,837,659 - Pf6N2E2_5062 0.84 -0.4
5,837,795 - +0.1
5,837,802 + -0.3
5,837,815 + +0.4
5,837,815 + -0.1
5,837,816 - +1.4
5,837,816 - -0.3
5,837,837 + -0.8
5,837,837 + -0.6
5,837,837 + +0.1
5,837,837 + -0.4
5,837,837 + +0.4
5,837,838 - +0.6
5,837,838 - -0.1
5,838,016 + +0.2
5,838,047 + +0.6
5,838,047 + +1.0
5,838,048 - +1.4
5,838,048 - +2.3
5,838,048 - -0.3
5,838,048 - +1.6
5,838,089 + +1.3
5,838,090 - +1.1
5,838,154 + Pf6N2E2_5063 0.13 +1.2
5,838,154 + Pf6N2E2_5063 0.13 +1.2
5,838,154 + Pf6N2E2_5063 0.13 +2.0
5,838,154 + Pf6N2E2_5063 0.13 +2.5
5,838,155 - Pf6N2E2_5063 0.13 +1.6
5,838,155 - Pf6N2E2_5063 0.13 +0.8
5,838,155 - Pf6N2E2_5063 0.13 +2.1
5,838,155 - Pf6N2E2_5063 0.13 +1.4
5,838,464 - Pf6N2E2_5063 0.42 +2.7
5,838,464 - Pf6N2E2_5063 0.42 +1.2
5,838,464 - Pf6N2E2_5063 0.42 +1.4
5,838,506 - Pf6N2E2_5063 0.45 +1.4
5,838,584 + Pf6N2E2_5063 0.53 +2.2
5,838,584 + Pf6N2E2_5063 0.53 +1.4
5,838,585 - Pf6N2E2_5063 0.53 +0.9
5,838,616 + Pf6N2E2_5063 0.56 +1.3
5,838,616 + Pf6N2E2_5063 0.56 +1.5
5,838,616 + Pf6N2E2_5063 0.56 +2.4
5,838,616 + Pf6N2E2_5063 0.56 +0.4
5,838,617 - Pf6N2E2_5063 0.56 +1.0
5,838,617 - Pf6N2E2_5063 0.56 +1.6
5,838,617 - Pf6N2E2_5063 0.56 +1.4
5,838,617 - Pf6N2E2_5063 0.56 +0.9
5,838,617 - Pf6N2E2_5063 0.56 +0.4
5,838,617 - Pf6N2E2_5063 0.56 +1.4
5,838,617 - Pf6N2E2_5063 0.56 -0.2
5,838,730 + Pf6N2E2_5063 0.66 +1.0
5,838,730 + Pf6N2E2_5063 0.66 +1.7
5,838,730 + Pf6N2E2_5063 0.66 +1.5
5,838,731 - Pf6N2E2_5063 0.66 +2.0
5,838,923 - Pf6N2E2_5063 0.84 +2.6
5,839,072 + +1.7
5,839,072 + +0.6
5,839,072 + +1.0
5,839,233 + Pf6N2E2_5064 0.11 +0.3
5,839,233 + Pf6N2E2_5064 0.11 +0.4
5,839,234 - Pf6N2E2_5064 0.11 +0.6
5,839,479 + Pf6N2E2_5064 0.30 +0.4
5,839,479 + Pf6N2E2_5064 0.30 +2.1
5,839,479 + Pf6N2E2_5064 0.30 +1.4
5,839,479 + Pf6N2E2_5064 0.30 +0.1
5,839,479 + Pf6N2E2_5064 0.30 +2.0
5,839,480 - Pf6N2E2_5064 0.30 -0.6
5,839,665 - Pf6N2E2_5064 0.44 +0.5

Or see this region's nucleotide sequence