Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3362

Experiment: Cobalt chloride 0.4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3360 and Pf6N2E2_3361 are separated by 106 nucleotidesPf6N2E2_3361 and Pf6N2E2_3362 are separated by 10 nucleotidesPf6N2E2_3362 and Pf6N2E2_3363 are separated by 28 nucleotidesPf6N2E2_3363 and Pf6N2E2_3364 overlap by 4 nucleotides Pf6N2E2_3360 - Heat shock protein FtsJ/RrmJ @ Ribosomal RNA large subunit methyltransferase E (EC 2.1.1.-), at 3,891,748 to 3,892,398 _3360 Pf6N2E2_3361 - FIG004454: RNA binding protein, at 3,892,505 to 3,892,813 _3361 Pf6N2E2_3362 - hypothetical protein, at 3,892,824 to 3,893,228 _3362 Pf6N2E2_3363 - Transcription elongation factor GreA, at 3,893,257 to 3,893,739 _3363 Pf6N2E2_3364 - Carbamoyl-phosphate synthase large chain (EC 6.3.5.5), at 3,893,736 to 3,896,957 _3364 Position (kb) 3892 3893 3894Strain fitness (log2 ratio) -2 -1 0 1at 3892.910 kb on - strand, within Pf6N2E2_3362at 3892.910 kb on - strand, within Pf6N2E2_3362at 3893.020 kb on - strand, within Pf6N2E2_3362at 3893.020 kb on - strand, within Pf6N2E2_3362at 3893.020 kb on - strand, within Pf6N2E2_3362at 3893.020 kb on - strand, within Pf6N2E2_3362at 3893.020 kb on - strand, within Pf6N2E2_3362at 3893.421 kb on - strand, within Pf6N2E2_3363at 3893.758 kb on - strandat 3893.758 kb on - strandat 3893.758 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM
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3,892,910 - Pf6N2E2_3362 0.21 -0.7
3,892,910 - Pf6N2E2_3362 0.21 -0.9
3,893,020 - Pf6N2E2_3362 0.48 +0.9
3,893,020 - Pf6N2E2_3362 0.48 -1.8
3,893,020 - Pf6N2E2_3362 0.48 -1.0
3,893,020 - Pf6N2E2_3362 0.48 -0.7
3,893,020 - Pf6N2E2_3362 0.48 -0.7
3,893,421 - Pf6N2E2_3363 0.34 -0.7
3,893,758 - +0.4
3,893,758 - -0.3
3,893,758 - -0.2

Or see this region's nucleotide sequence