Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2206 and Pf6N2E2_2207 are separated by 126 nucleotides Pf6N2E2_2207 and Pf6N2E2_2208 are separated by 6 nucleotides
Pf6N2E2_2206 - VgrG protein, at 2,612,696 to 2,614,102
_2206
Pf6N2E2_2207 - cytochrome b(561), at 2,614,229 to 2,614,777
_2207
Pf6N2E2_2208 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 2,614,784 to 2,615,692
_2208
Position (kb)
2614
2615 Strain fitness (log2 ratio)
-1
0
1
2 at 2613.323 kb on + strand, within Pf6N2E2_2206 at 2613.324 kb on - strand, within Pf6N2E2_2206 at 2613.324 kb on - strand, within Pf6N2E2_2206 at 2613.535 kb on + strand, within Pf6N2E2_2206 at 2613.536 kb on - strand, within Pf6N2E2_2206 at 2613.567 kb on + strand, within Pf6N2E2_2206 at 2613.567 kb on + strand, within Pf6N2E2_2206 at 2613.789 kb on + strand, within Pf6N2E2_2206 at 2613.789 kb on + strand, within Pf6N2E2_2206 at 2613.790 kb on - strand, within Pf6N2E2_2206 at 2613.790 kb on - strand, within Pf6N2E2_2206 at 2613.790 kb on - strand, within Pf6N2E2_2206 at 2613.849 kb on + strand, within Pf6N2E2_2206 at 2614.111 kb on + strand at 2614.111 kb on + strand at 2614.112 kb on - strand at 2614.167 kb on + strand at 2614.192 kb on - strand at 2614.192 kb on - strand at 2614.192 kb on - strand at 2614.256 kb on + strand at 2614.256 kb on + strand at 2614.261 kb on + strand at 2614.422 kb on + strand, within Pf6N2E2_2207 at 2614.423 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.592 kb on + strand, within Pf6N2E2_2207 at 2614.593 kb on - strand, within Pf6N2E2_2207 at 2614.727 kb on + strand at 2614.727 kb on + strand at 2614.728 kb on - strand at 2614.728 kb on - strand at 2614.728 kb on - strand at 2614.902 kb on + strand, within Pf6N2E2_2208 at 2614.903 kb on - strand, within Pf6N2E2_2208 at 2615.001 kb on + strand, within Pf6N2E2_2208 at 2615.001 kb on + strand, within Pf6N2E2_2208 at 2615.001 kb on + strand, within Pf6N2E2_2208 at 2615.340 kb on + strand, within Pf6N2E2_2208 at 2615.481 kb on + strand, within Pf6N2E2_2208 at 2615.481 kb on + strand, within Pf6N2E2_2208 at 2615.726 kb on + strand at 2615.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 2,613,323 + Pf6N2E2_2206 0.45 -0.6 2,613,324 - Pf6N2E2_2206 0.45 -0.3 2,613,324 - Pf6N2E2_2206 0.45 +0.2 2,613,535 + Pf6N2E2_2206 0.60 -0.5 2,613,536 - Pf6N2E2_2206 0.60 -0.9 2,613,567 + Pf6N2E2_2206 0.62 +0.9 2,613,567 + Pf6N2E2_2206 0.62 -1.1 2,613,789 + Pf6N2E2_2206 0.78 +1.2 2,613,789 + Pf6N2E2_2206 0.78 -0.2 2,613,790 - Pf6N2E2_2206 0.78 +0.3 2,613,790 - Pf6N2E2_2206 0.78 -0.4 2,613,790 - Pf6N2E2_2206 0.78 +0.5 2,613,849 + Pf6N2E2_2206 0.82 +0.1 2,614,111 + -1.4 2,614,111 + +1.9 2,614,112 - +0.4 2,614,167 + -0.6 2,614,192 - +0.6 2,614,192 - -0.7 2,614,192 - +0.3 2,614,256 + -0.1 2,614,256 + -0.4 2,614,261 + -0.4 2,614,422 + Pf6N2E2_2207 0.35 -0.2 2,614,423 - Pf6N2E2_2207 0.35 -0.9 2,614,521 - Pf6N2E2_2207 0.53 -0.6 2,614,521 - Pf6N2E2_2207 0.53 -0.6 2,614,521 - Pf6N2E2_2207 0.53 -1.1 2,614,521 - Pf6N2E2_2207 0.53 -0.1 2,614,521 - Pf6N2E2_2207 0.53 -1.3 2,614,592 + Pf6N2E2_2207 0.66 -0.9 2,614,593 - Pf6N2E2_2207 0.66 +0.4 2,614,727 + -0.1 2,614,727 + +0.2 2,614,728 - -0.1 2,614,728 - +0.4 2,614,728 - -1.6 2,614,902 + Pf6N2E2_2208 0.13 -0.6 2,614,903 - Pf6N2E2_2208 0.13 +0.5 2,615,001 + Pf6N2E2_2208 0.24 +1.0 2,615,001 + Pf6N2E2_2208 0.24 +0.1 2,615,001 + Pf6N2E2_2208 0.24 +2.5 2,615,340 + Pf6N2E2_2208 0.61 +0.4 2,615,481 + Pf6N2E2_2208 0.77 -0.4 2,615,481 + Pf6N2E2_2208 0.77 +0.3 2,615,726 + +0.1 2,615,727 - -0.2
Or see this region's nucleotide sequence