Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1999 and Pf6N2E2_2000 are separated by 585 nucleotides Pf6N2E2_2000 and Pf6N2E2_2001 are separated by 576 nucleotides
Pf6N2E2_1999 - putative membrane protein, at 2,365,743 to 2,367,248
_1999
Pf6N2E2_2000 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 2,367,834 to 2,369,675
_2000
Pf6N2E2_2001 - Nuclease precursor (EC 3.1.30.2), at 2,370,252 to 2,371,277
_2001
Position (kb)
2367
2368
2369
2370 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2366.893 kb on - strand, within Pf6N2E2_1999 at 2366.893 kb on - strand, within Pf6N2E2_1999 at 2366.893 kb on - strand, within Pf6N2E2_1999 at 2367.044 kb on - strand, within Pf6N2E2_1999 at 2367.081 kb on + strand, within Pf6N2E2_1999 at 2367.081 kb on + strand, within Pf6N2E2_1999 at 2367.082 kb on - strand, within Pf6N2E2_1999 at 2367.082 kb on - strand, within Pf6N2E2_1999 at 2367.082 kb on - strand, within Pf6N2E2_1999 at 2367.082 kb on - strand, within Pf6N2E2_1999 at 2367.107 kb on - strand at 2367.110 kb on - strand at 2367.142 kb on + strand at 2367.142 kb on + strand at 2367.143 kb on - strand at 2367.176 kb on + strand at 2367.177 kb on - strand at 2367.177 kb on - strand at 2367.177 kb on - strand at 2367.182 kb on + strand at 2367.182 kb on + strand at 2367.182 kb on + strand at 2367.183 kb on - strand at 2367.185 kb on + strand at 2367.185 kb on + strand at 2367.186 kb on - strand at 2367.188 kb on + strand at 2367.188 kb on + strand at 2367.189 kb on - strand at 2367.189 kb on - strand at 2367.234 kb on - strand at 2367.242 kb on + strand at 2367.246 kb on + strand at 2367.247 kb on - strand at 2367.285 kb on + strand at 2367.286 kb on - strand at 2367.286 kb on - strand at 2367.286 kb on - strand at 2367.305 kb on + strand at 2367.305 kb on + strand at 2367.305 kb on + strand at 2367.306 kb on - strand at 2367.320 kb on + strand at 2367.320 kb on + strand at 2367.412 kb on + strand at 2367.412 kb on + strand at 2367.434 kb on - strand at 2367.448 kb on + strand at 2367.475 kb on - strand at 2367.475 kb on - strand at 2367.511 kb on - strand at 2367.544 kb on - strand at 2367.544 kb on - strand at 2367.554 kb on - strand at 2367.554 kb on - strand at 2367.610 kb on - strand at 2367.629 kb on + strand at 2367.629 kb on + strand at 2367.629 kb on + strand at 2367.629 kb on + strand at 2367.630 kb on - strand at 2367.630 kb on - strand at 2367.649 kb on - strand at 2367.684 kb on + strand at 2367.709 kb on - strand at 2367.709 kb on - strand at 2367.716 kb on - strand at 2367.730 kb on + strand at 2367.976 kb on + strand at 2367.977 kb on - strand at 2367.977 kb on - strand at 2367.977 kb on - strand at 2367.977 kb on - strand at 2367.977 kb on - strand at 2368.024 kb on - strand, within Pf6N2E2_2000 at 2368.137 kb on + strand, within Pf6N2E2_2000 at 2368.138 kb on - strand, within Pf6N2E2_2000 at 2368.145 kb on - strand, within Pf6N2E2_2000 at 2368.234 kb on - strand, within Pf6N2E2_2000 at 2368.270 kb on + strand, within Pf6N2E2_2000 at 2368.271 kb on - strand, within Pf6N2E2_2000 at 2368.288 kb on - strand, within Pf6N2E2_2000 at 2368.288 kb on - strand, within Pf6N2E2_2000 at 2368.456 kb on + strand, within Pf6N2E2_2000 at 2368.457 kb on - strand, within Pf6N2E2_2000 at 2368.571 kb on + strand, within Pf6N2E2_2000 at 2368.610 kb on + strand, within Pf6N2E2_2000 at 2368.611 kb on - strand, within Pf6N2E2_2000 at 2368.611 kb on - strand, within Pf6N2E2_2000 at 2368.716 kb on - strand, within Pf6N2E2_2000 at 2368.736 kb on - strand, within Pf6N2E2_2000 at 2368.896 kb on - strand, within Pf6N2E2_2000 at 2368.896 kb on - strand, within Pf6N2E2_2000 at 2368.976 kb on - strand, within Pf6N2E2_2000 at 2369.016 kb on - strand, within Pf6N2E2_2000 at 2369.016 kb on - strand, within Pf6N2E2_2000 at 2369.016 kb on - strand, within Pf6N2E2_2000 at 2369.016 kb on - strand, within Pf6N2E2_2000 at 2369.303 kb on + strand, within Pf6N2E2_2000 at 2369.304 kb on - strand, within Pf6N2E2_2000 at 2369.304 kb on - strand, within Pf6N2E2_2000 at 2369.333 kb on + strand, within Pf6N2E2_2000 at 2369.333 kb on + strand, within Pf6N2E2_2000 at 2369.333 kb on + strand, within Pf6N2E2_2000 at 2369.334 kb on - strand, within Pf6N2E2_2000 at 2369.334 kb on - strand, within Pf6N2E2_2000 at 2369.334 kb on - strand, within Pf6N2E2_2000 at 2369.334 kb on - strand, within Pf6N2E2_2000 at 2369.334 kb on - strand, within Pf6N2E2_2000 at 2369.334 kb on - strand, within Pf6N2E2_2000 at 2369.334 kb on - strand, within Pf6N2E2_2000 at 2369.352 kb on + strand, within Pf6N2E2_2000 at 2369.352 kb on + strand, within Pf6N2E2_2000 at 2369.353 kb on - strand, within Pf6N2E2_2000 at 2369.377 kb on + strand, within Pf6N2E2_2000 at 2369.378 kb on - strand, within Pf6N2E2_2000 at 2369.378 kb on - strand, within Pf6N2E2_2000 at 2369.382 kb on + strand, within Pf6N2E2_2000 at 2369.382 kb on + strand, within Pf6N2E2_2000 at 2369.504 kb on + strand at 2369.611 kb on - strand at 2369.716 kb on + strand at 2369.716 kb on + strand at 2369.717 kb on - strand at 2369.746 kb on - strand at 2369.746 kb on - strand at 2369.746 kb on - strand at 2369.848 kb on + strand at 2369.871 kb on + strand at 2369.935 kb on - strand at 2369.949 kb on + strand at 2369.950 kb on - strand at 2369.950 kb on - strand at 2369.950 kb on - strand at 2370.035 kb on - strand at 2370.157 kb on + strand at 2370.202 kb on + strand at 2370.202 kb on + strand at 2370.202 kb on + strand at 2370.202 kb on + strand at 2370.203 kb on - strand at 2370.203 kb on - strand at 2370.203 kb on - strand at 2370.209 kb on - strand at 2370.254 kb on - strand at 2370.345 kb on + strand at 2370.346 kb on - strand at 2370.346 kb on - strand at 2370.388 kb on + strand, within Pf6N2E2_2001 at 2370.388 kb on + strand, within Pf6N2E2_2001 at 2370.389 kb on - strand, within Pf6N2E2_2001 at 2370.457 kb on + strand, within Pf6N2E2_2001 at 2370.477 kb on - strand, within Pf6N2E2_2001 at 2370.562 kb on + strand, within Pf6N2E2_2001 at 2370.583 kb on + strand, within Pf6N2E2_2001 at 2370.583 kb on + strand, within Pf6N2E2_2001 at 2370.584 kb on - strand, within Pf6N2E2_2001 at 2370.611 kb on + strand, within Pf6N2E2_2001
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 2,366,893 - Pf6N2E2_1999 0.76 +0.5 2,366,893 - Pf6N2E2_1999 0.76 +1.5 2,366,893 - Pf6N2E2_1999 0.76 -0.4 2,367,044 - Pf6N2E2_1999 0.86 -1.3 2,367,081 + Pf6N2E2_1999 0.89 +0.3 2,367,081 + Pf6N2E2_1999 0.89 +0.9 2,367,082 - Pf6N2E2_1999 0.89 -1.5 2,367,082 - Pf6N2E2_1999 0.89 +1.3 2,367,082 - Pf6N2E2_1999 0.89 -0.4 2,367,082 - Pf6N2E2_1999 0.89 +0.4 2,367,107 - -1.0 2,367,110 - +0.9 2,367,142 + +0.9 2,367,142 + +0.6 2,367,143 - -0.1 2,367,176 + -0.5 2,367,177 - +0.2 2,367,177 - +0.3 2,367,177 - -0.7 2,367,182 + -0.8 2,367,182 + +0.3 2,367,182 + +0.9 2,367,183 - +0.4 2,367,185 + +1.1 2,367,185 + +0.3 2,367,186 - -0.1 2,367,188 + +0.9 2,367,188 + +1.5 2,367,189 - -0.7 2,367,189 - -0.6 2,367,234 - +1.7 2,367,242 + +1.9 2,367,246 + -0.9 2,367,247 - +0.9 2,367,285 + -0.9 2,367,286 - -0.9 2,367,286 - -1.1 2,367,286 - +2.1 2,367,305 + +0.3 2,367,305 + +0.6 2,367,305 + -0.2 2,367,306 - -0.7 2,367,320 + +0.2 2,367,320 + -0.6 2,367,412 + -0.7 2,367,412 + -0.1 2,367,434 - +0.9 2,367,448 + -0.1 2,367,475 - -0.1 2,367,475 - +0.6 2,367,511 - -0.1 2,367,544 - -0.1 2,367,544 - -0.3 2,367,554 - +0.5 2,367,554 - +0.5 2,367,610 - +0.6 2,367,629 + -0.1 2,367,629 + -0.4 2,367,629 + +0.2 2,367,629 + -1.1 2,367,630 - +1.5 2,367,630 - -1.4 2,367,649 - -0.3 2,367,684 + +0.6 2,367,709 - +0.5 2,367,709 - +0.9 2,367,716 - +1.9 2,367,730 + +0.9 2,367,976 + -0.1 2,367,977 - +0.8 2,367,977 - +1.0 2,367,977 - -0.7 2,367,977 - -0.9 2,367,977 - -1.7 2,368,024 - Pf6N2E2_2000 0.10 -0.4 2,368,137 + Pf6N2E2_2000 0.16 -0.7 2,368,138 - Pf6N2E2_2000 0.17 +0.5 2,368,145 - Pf6N2E2_2000 0.17 +0.9 2,368,234 - Pf6N2E2_2000 0.22 +0.7 2,368,270 + Pf6N2E2_2000 0.24 +0.9 2,368,271 - Pf6N2E2_2000 0.24 +0.6 2,368,288 - Pf6N2E2_2000 0.25 +0.5 2,368,288 - Pf6N2E2_2000 0.25 -1.3 2,368,456 + Pf6N2E2_2000 0.34 +1.9 2,368,457 - Pf6N2E2_2000 0.34 -0.8 2,368,571 + Pf6N2E2_2000 0.40 -1.7 2,368,610 + Pf6N2E2_2000 0.42 -1.7 2,368,611 - Pf6N2E2_2000 0.42 -1.4 2,368,611 - Pf6N2E2_2000 0.42 +0.5 2,368,716 - Pf6N2E2_2000 0.48 -0.4 2,368,736 - Pf6N2E2_2000 0.49 +0.9 2,368,896 - Pf6N2E2_2000 0.58 -0.9 2,368,896 - Pf6N2E2_2000 0.58 -1.9 2,368,976 - Pf6N2E2_2000 0.62 +0.9 2,369,016 - Pf6N2E2_2000 0.64 +0.9 2,369,016 - Pf6N2E2_2000 0.64 +0.3 2,369,016 - Pf6N2E2_2000 0.64 +0.2 2,369,016 - Pf6N2E2_2000 0.64 -0.7 2,369,303 + Pf6N2E2_2000 0.80 -2.7 2,369,304 - Pf6N2E2_2000 0.80 +0.3 2,369,304 - Pf6N2E2_2000 0.80 -0.1 2,369,333 + Pf6N2E2_2000 0.81 -0.1 2,369,333 + Pf6N2E2_2000 0.81 +0.9 2,369,333 + Pf6N2E2_2000 0.81 +0.9 2,369,334 - Pf6N2E2_2000 0.81 -0.7 2,369,334 - Pf6N2E2_2000 0.81 +0.9 2,369,334 - Pf6N2E2_2000 0.81 +1.9 2,369,334 - Pf6N2E2_2000 0.81 -1.5 2,369,334 - Pf6N2E2_2000 0.81 -0.1 2,369,334 - Pf6N2E2_2000 0.81 +0.2 2,369,334 - Pf6N2E2_2000 0.81 -1.4 2,369,352 + Pf6N2E2_2000 0.82 -0.4 2,369,352 + Pf6N2E2_2000 0.82 +1.3 2,369,353 - Pf6N2E2_2000 0.82 -0.1 2,369,377 + Pf6N2E2_2000 0.84 -1.2 2,369,378 - Pf6N2E2_2000 0.84 -0.7 2,369,378 - Pf6N2E2_2000 0.84 -0.7 2,369,382 + Pf6N2E2_2000 0.84 +1.5 2,369,382 + Pf6N2E2_2000 0.84 +0.4 2,369,504 + -1.1 2,369,611 - +1.2 2,369,716 + -0.1 2,369,716 + -0.1 2,369,717 - -0.1 2,369,746 - +0.7 2,369,746 - -0.7 2,369,746 - +0.2 2,369,848 + -0.8 2,369,871 + -0.7 2,369,935 - +1.9 2,369,949 + -0.5 2,369,950 - +0.0 2,369,950 - -0.2 2,369,950 - +0.3 2,370,035 - +0.2 2,370,157 + -0.2 2,370,202 + -2.2 2,370,202 + +1.5 2,370,202 + -1.0 2,370,202 + +0.2 2,370,203 - +0.8 2,370,203 - -0.1 2,370,203 - +0.4 2,370,209 - +0.1 2,370,254 - -0.4 2,370,345 + +0.5 2,370,346 - -1.4 2,370,346 - -0.2 2,370,388 + Pf6N2E2_2001 0.13 -0.7 2,370,388 + Pf6N2E2_2001 0.13 -1.9 2,370,389 - Pf6N2E2_2001 0.13 +0.6 2,370,457 + Pf6N2E2_2001 0.20 +0.2 2,370,477 - Pf6N2E2_2001 0.22 -0.6 2,370,562 + Pf6N2E2_2001 0.30 +0.3 2,370,583 + Pf6N2E2_2001 0.32 +0.4 2,370,583 + Pf6N2E2_2001 0.32 +1.3 2,370,584 - Pf6N2E2_2001 0.32 -0.3 2,370,611 + Pf6N2E2_2001 0.35 +0.3
Or see this region's nucleotide sequence