Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1503 and Pf6N2E2_1504 are separated by 109 nucleotides Pf6N2E2_1504 and Pf6N2E2_1505 are separated by 28 nucleotides Pf6N2E2_1505 and Pf6N2E2_1506 are separated by 215 nucleotides
Pf6N2E2_1503 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 1,755,943 to 1,758,126
_1503
Pf6N2E2_1504 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 1,758,236 to 1,759,066
_1504
Pf6N2E2_1505 - Transporter, MFS superfamily, at 1,759,095 to 1,760,291
_1505
Pf6N2E2_1506 - Putative alkanesulfonate metabolism utilization regulator, at 1,760,507 to 1,761,238
_1506
Position (kb)
1759
1760
1761 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1758.107 kb on - strand at 1758.181 kb on + strand at 1758.181 kb on + strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.189 kb on + strand at 1758.190 kb on - strand at 1758.472 kb on - strand, within Pf6N2E2_1504 at 1758.943 kb on - strand, within Pf6N2E2_1504 at 1758.943 kb on - strand, within Pf6N2E2_1504 at 1758.963 kb on + strand, within Pf6N2E2_1504 at 1758.963 kb on + strand, within Pf6N2E2_1504 at 1759.048 kb on + strand at 1759.051 kb on + strand at 1759.051 kb on + strand at 1759.051 kb on + strand at 1759.051 kb on + strand at 1759.052 kb on - strand at 1759.315 kb on + strand, within Pf6N2E2_1505 at 1759.316 kb on - strand, within Pf6N2E2_1505 at 1759.316 kb on - strand, within Pf6N2E2_1505 at 1759.378 kb on - strand, within Pf6N2E2_1505 at 1759.462 kb on + strand, within Pf6N2E2_1505 at 1759.462 kb on + strand, within Pf6N2E2_1505 at 1759.463 kb on - strand, within Pf6N2E2_1505 at 1759.463 kb on - strand, within Pf6N2E2_1505 at 1759.463 kb on - strand, within Pf6N2E2_1505 at 1759.588 kb on + strand, within Pf6N2E2_1505 at 1759.901 kb on - strand, within Pf6N2E2_1505 at 1759.957 kb on - strand, within Pf6N2E2_1505 at 1759.957 kb on - strand, within Pf6N2E2_1505 at 1760.158 kb on + strand, within Pf6N2E2_1505 at 1760.295 kb on + strand at 1760.295 kb on + strand at 1760.296 kb on - strand at 1760.296 kb on - strand at 1760.296 kb on - strand at 1760.296 kb on - strand at 1760.650 kb on - strand, within Pf6N2E2_1506 at 1760.721 kb on + strand, within Pf6N2E2_1506 at 1760.722 kb on - strand, within Pf6N2E2_1506 at 1760.849 kb on - strand, within Pf6N2E2_1506 at 1761.105 kb on + strand, within Pf6N2E2_1506 at 1761.106 kb on - strand, within Pf6N2E2_1506 at 1761.201 kb on + strand at 1761.202 kb on - strand at 1761.246 kb on - strand at 1761.269 kb on + strand at 1761.272 kb on - strand at 1761.289 kb on + strand at 1761.289 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 1,758,107 - -3.1 1,758,181 + -0.8 1,758,181 + -0.8 1,758,182 - +0.8 1,758,182 - -0.2 1,758,182 - +1.8 1,758,182 - +0.2 1,758,182 - -0.8 1,758,182 - -0.8 1,758,189 + -0.2 1,758,190 - -0.1 1,758,472 - Pf6N2E2_1504 0.28 +2.3 1,758,943 - Pf6N2E2_1504 0.85 +0.9 1,758,943 - Pf6N2E2_1504 0.85 +0.0 1,758,963 + Pf6N2E2_1504 0.87 +0.3 1,758,963 + Pf6N2E2_1504 0.87 +0.0 1,759,048 + +0.5 1,759,051 + -0.0 1,759,051 + -0.8 1,759,051 + +1.3 1,759,051 + +0.8 1,759,052 - +0.5 1,759,315 + Pf6N2E2_1505 0.18 +0.2 1,759,316 - Pf6N2E2_1505 0.18 -0.2 1,759,316 - Pf6N2E2_1505 0.18 -0.6 1,759,378 - Pf6N2E2_1505 0.24 -0.2 1,759,462 + Pf6N2E2_1505 0.31 -0.6 1,759,462 + Pf6N2E2_1505 0.31 -0.6 1,759,463 - Pf6N2E2_1505 0.31 -1.2 1,759,463 - Pf6N2E2_1505 0.31 -2.4 1,759,463 - Pf6N2E2_1505 0.31 +0.4 1,759,588 + Pf6N2E2_1505 0.41 -0.2 1,759,901 - Pf6N2E2_1505 0.67 +1.3 1,759,957 - Pf6N2E2_1505 0.72 -0.4 1,759,957 - Pf6N2E2_1505 0.72 -1.8 1,760,158 + Pf6N2E2_1505 0.89 -0.6 1,760,295 + -0.2 1,760,295 + +0.8 1,760,296 - +1.2 1,760,296 - +0.8 1,760,296 - +0.4 1,760,296 - -0.2 1,760,650 - Pf6N2E2_1506 0.20 +1.5 1,760,721 + Pf6N2E2_1506 0.29 -1.2 1,760,722 - Pf6N2E2_1506 0.29 -0.6 1,760,849 - Pf6N2E2_1506 0.47 +1.4 1,761,105 + Pf6N2E2_1506 0.82 +0.8 1,761,106 - Pf6N2E2_1506 0.82 -1.2 1,761,201 + +1.8 1,761,202 - +0.8 1,761,246 - +1.5 1,761,269 + +0.2 1,761,272 - -2.4 1,761,289 + -0.5 1,761,289 + +1.5
Or see this region's nucleotide sequence