Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1153 and Pf6N2E2_1154 are separated by 77 nucleotides Pf6N2E2_1154 and Pf6N2E2_1155 are separated by 294 nucleotides Pf6N2E2_1155 and Pf6N2E2_1156 overlap by 29 nucleotides Pf6N2E2_1156 and Pf6N2E2_1157 are separated by 39 nucleotides
Pf6N2E2_1153 - small molecule metabolism; degradation; fatty acid, at 1,350,924 to 1,351,739
_1153
Pf6N2E2_1154 - FIG00978082: hypothetical protein, at 1,351,817 to 1,352,404
_1154
Pf6N2E2_1155 - Cyn operon transcriptional activator, at 1,352,699 to 1,353,706
_1155
Pf6N2E2_1156 - hypothetical protein, at 1,353,678 to 1,354,211
_1156
Pf6N2E2_1157 - hypothetical protein possibly connected to lactam utilization and allophanate hydrolase, at 1,354,251 to 1,355,042
_1157
Position (kb)
1352
1353
1354 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1351.718 kb on + strand at 1351.743 kb on + strand at 1351.743 kb on + strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.747 kb on + strand at 1351.748 kb on - strand at 1351.815 kb on - strand at 1351.904 kb on + strand, within Pf6N2E2_1154 at 1351.905 kb on - strand, within Pf6N2E2_1154 at 1351.957 kb on - strand, within Pf6N2E2_1154 at 1351.957 kb on - strand, within Pf6N2E2_1154 at 1351.957 kb on - strand, within Pf6N2E2_1154 at 1352.001 kb on + strand, within Pf6N2E2_1154 at 1352.001 kb on + strand, within Pf6N2E2_1154 at 1352.001 kb on + strand, within Pf6N2E2_1154 at 1352.002 kb on - strand, within Pf6N2E2_1154 at 1352.052 kb on + strand, within Pf6N2E2_1154 at 1352.053 kb on - strand, within Pf6N2E2_1154 at 1352.122 kb on - strand, within Pf6N2E2_1154 at 1352.122 kb on - strand, within Pf6N2E2_1154 at 1352.122 kb on - strand, within Pf6N2E2_1154 at 1352.293 kb on + strand, within Pf6N2E2_1154 at 1352.294 kb on - strand, within Pf6N2E2_1154 at 1352.495 kb on + strand at 1352.495 kb on + strand at 1352.495 kb on + strand at 1352.495 kb on + strand at 1352.496 kb on - strand at 1352.496 kb on - strand at 1352.496 kb on - strand at 1352.496 kb on - strand at 1352.564 kb on - strand at 1352.655 kb on + strand at 1352.655 kb on + strand at 1352.655 kb on + strand at 1352.655 kb on + strand at 1352.656 kb on - strand at 1352.656 kb on - strand at 1352.656 kb on - strand at 1352.672 kb on + strand at 1352.672 kb on + strand at 1352.672 kb on + strand at 1352.672 kb on + strand at 1353.053 kb on + strand, within Pf6N2E2_1155 at 1353.087 kb on + strand, within Pf6N2E2_1155 at 1353.088 kb on - strand, within Pf6N2E2_1155 at 1353.158 kb on + strand, within Pf6N2E2_1155 at 1353.158 kb on + strand, within Pf6N2E2_1155 at 1353.158 kb on + strand, within Pf6N2E2_1155 at 1353.158 kb on + strand, within Pf6N2E2_1155 at 1353.159 kb on - strand, within Pf6N2E2_1155 at 1353.159 kb on - strand, within Pf6N2E2_1155 at 1353.159 kb on - strand, within Pf6N2E2_1155 at 1353.159 kb on - strand, within Pf6N2E2_1155 at 1353.159 kb on - strand, within Pf6N2E2_1155 at 1353.420 kb on + strand, within Pf6N2E2_1155 at 1353.421 kb on - strand, within Pf6N2E2_1155 at 1353.889 kb on + strand, within Pf6N2E2_1156 at 1353.890 kb on - strand, within Pf6N2E2_1156 at 1353.890 kb on - strand, within Pf6N2E2_1156 at 1354.003 kb on + strand, within Pf6N2E2_1156 at 1354.130 kb on + strand, within Pf6N2E2_1156 at 1354.130 kb on + strand, within Pf6N2E2_1156 at 1354.180 kb on + strand at 1354.180 kb on + strand at 1354.180 kb on + strand at 1354.181 kb on - strand at 1354.253 kb on - strand at 1354.270 kb on - strand at 1354.350 kb on + strand, within Pf6N2E2_1157 at 1354.350 kb on + strand, within Pf6N2E2_1157 at 1354.408 kb on + strand, within Pf6N2E2_1157 at 1354.461 kb on + strand, within Pf6N2E2_1157
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 1,351,718 + +0.7 1,351,743 + -0.1 1,351,743 + -0.3 1,351,744 - -1.3 1,351,744 - -0.3 1,351,744 - +0.1 1,351,744 - +1.7 1,351,744 - +0.2 1,351,747 + +0.7 1,351,748 - -0.9 1,351,815 - +0.3 1,351,904 + Pf6N2E2_1154 0.15 -0.3 1,351,905 - Pf6N2E2_1154 0.15 -0.3 1,351,957 - Pf6N2E2_1154 0.24 +1.7 1,351,957 - Pf6N2E2_1154 0.24 +1.7 1,351,957 - Pf6N2E2_1154 0.24 +1.3 1,352,001 + Pf6N2E2_1154 0.31 -1.1 1,352,001 + Pf6N2E2_1154 0.31 +0.4 1,352,001 + Pf6N2E2_1154 0.31 +0.3 1,352,002 - Pf6N2E2_1154 0.31 +0.1 1,352,052 + Pf6N2E2_1154 0.40 +0.1 1,352,053 - Pf6N2E2_1154 0.40 -0.7 1,352,122 - Pf6N2E2_1154 0.52 +0.7 1,352,122 - Pf6N2E2_1154 0.52 -0.9 1,352,122 - Pf6N2E2_1154 0.52 +0.7 1,352,293 + Pf6N2E2_1154 0.81 -0.6 1,352,294 - Pf6N2E2_1154 0.81 +0.7 1,352,495 + +0.7 1,352,495 + +1.3 1,352,495 + -0.9 1,352,495 + +0.6 1,352,496 - -1.2 1,352,496 - -1.2 1,352,496 - +2.1 1,352,496 - +0.7 1,352,564 - -0.1 1,352,655 + -0.3 1,352,655 + +0.3 1,352,655 + -0.6 1,352,655 + +0.3 1,352,656 - +0.8 1,352,656 - -0.1 1,352,656 - +2.3 1,352,672 + -1.5 1,352,672 + +0.2 1,352,672 + -0.2 1,352,672 + -0.3 1,353,053 + Pf6N2E2_1155 0.35 -2.1 1,353,087 + Pf6N2E2_1155 0.38 -1.1 1,353,088 - Pf6N2E2_1155 0.39 -0.3 1,353,158 + Pf6N2E2_1155 0.46 +0.4 1,353,158 + Pf6N2E2_1155 0.46 +0.7 1,353,158 + Pf6N2E2_1155 0.46 -0.3 1,353,158 + Pf6N2E2_1155 0.46 -2.3 1,353,159 - Pf6N2E2_1155 0.46 -2.3 1,353,159 - Pf6N2E2_1155 0.46 -0.9 1,353,159 - Pf6N2E2_1155 0.46 +0.3 1,353,159 - Pf6N2E2_1155 0.46 -0.3 1,353,159 - Pf6N2E2_1155 0.46 +1.7 1,353,420 + Pf6N2E2_1155 0.72 -1.5 1,353,421 - Pf6N2E2_1155 0.72 +0.7 1,353,889 + Pf6N2E2_1156 0.40 -0.9 1,353,890 - Pf6N2E2_1156 0.40 +0.3 1,353,890 - Pf6N2E2_1156 0.40 -0.9 1,354,003 + Pf6N2E2_1156 0.61 -0.9 1,354,130 + Pf6N2E2_1156 0.85 +1.3 1,354,130 + Pf6N2E2_1156 0.85 -0.5 1,354,180 + +0.5 1,354,180 + -0.6 1,354,180 + +0.0 1,354,181 - -0.3 1,354,253 - -0.5 1,354,270 - +0.7 1,354,350 + Pf6N2E2_1157 0.12 -1.9 1,354,350 + Pf6N2E2_1157 0.12 +0.9 1,354,408 + Pf6N2E2_1157 0.20 -1.1 1,354,461 + Pf6N2E2_1157 0.27 -0.8
Or see this region's nucleotide sequence