Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2855 and Pf6N2E2_2856 are separated by 67 nucleotides Pf6N2E2_2856 and Pf6N2E2_2857 are separated by 140 nucleotides Pf6N2E2_2857 and Pf6N2E2_2858 are separated by 32 nucleotides Pf6N2E2_2858 and Pf6N2E2_2859 are separated by 11 nucleotides
Pf6N2E2_2855 - DGPFAETKE, at 3,347,764 to 3,348,108
_2855
Pf6N2E2_2856 - Ketosteroid isomerase homolog, at 3,348,176 to 3,348,610
_2856
Pf6N2E2_2857 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,348,751 to 3,349,236
_2857
Pf6N2E2_2858 - Phosphohydrolase (MutT/nudix family protein), at 3,349,269 to 3,349,877
_2858
Pf6N2E2_2859 - Bacterial transcription activator, effector binding protein, at 3,349,889 to 3,350,377
_2859
Position (kb)
3348
3349
3350 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3347.754 kb on + strand at 3347.754 kb on + strand at 3347.754 kb on + strand at 3347.754 kb on + strand at 3347.755 kb on - strand at 3347.755 kb on - strand at 3347.755 kb on - strand at 3347.755 kb on - strand at 3347.758 kb on + strand at 3347.758 kb on + strand at 3347.874 kb on - strand, within Pf6N2E2_2855 at 3347.874 kb on - strand, within Pf6N2E2_2855 at 3347.874 kb on - strand, within Pf6N2E2_2855 at 3347.874 kb on - strand, within Pf6N2E2_2855 at 3348.023 kb on + strand, within Pf6N2E2_2855 at 3348.084 kb on + strand at 3348.084 kb on + strand at 3348.084 kb on + strand at 3348.085 kb on - strand at 3348.085 kb on - strand at 3348.085 kb on - strand at 3348.085 kb on - strand at 3348.086 kb on + strand at 3348.086 kb on + strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.102 kb on - strand at 3348.102 kb on - strand at 3348.102 kb on - strand at 3348.102 kb on - strand at 3348.137 kb on + strand at 3348.137 kb on + strand at 3348.137 kb on + strand at 3348.138 kb on - strand at 3348.138 kb on - strand at 3348.138 kb on - strand at 3348.138 kb on - strand at 3348.146 kb on + strand at 3348.146 kb on + strand at 3348.146 kb on + strand at 3348.147 kb on - strand at 3348.185 kb on + strand at 3348.185 kb on + strand at 3348.185 kb on + strand at 3348.186 kb on - strand at 3348.186 kb on - strand at 3348.337 kb on - strand, within Pf6N2E2_2856 at 3348.337 kb on - strand, within Pf6N2E2_2856 at 3348.513 kb on + strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.561 kb on + strand, within Pf6N2E2_2856 at 3348.562 kb on - strand, within Pf6N2E2_2856 at 3348.604 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.723 kb on - strand at 3349.042 kb on + strand, within Pf6N2E2_2857 at 3349.153 kb on + strand, within Pf6N2E2_2857 at 3349.153 kb on + strand, within Pf6N2E2_2857 at 3349.154 kb on - strand, within Pf6N2E2_2857 at 3349.154 kb on - strand, within Pf6N2E2_2857 at 3349.190 kb on - strand at 3349.190 kb on - strand at 3349.252 kb on - strand at 3349.305 kb on + strand at 3349.330 kb on + strand, within Pf6N2E2_2858 at 3349.330 kb on + strand, within Pf6N2E2_2858 at 3349.362 kb on + strand, within Pf6N2E2_2858 at 3349.363 kb on - strand, within Pf6N2E2_2858 at 3349.363 kb on - strand, within Pf6N2E2_2858 at 3349.428 kb on - strand, within Pf6N2E2_2858 at 3349.833 kb on + strand at 3349.833 kb on + strand at 3349.834 kb on - strand at 3349.834 kb on - strand at 3349.873 kb on - strand at 3349.873 kb on - strand at 3349.959 kb on + strand, within Pf6N2E2_2859 at 3349.990 kb on + strand, within Pf6N2E2_2859 at 3349.991 kb on - strand, within Pf6N2E2_2859 at 3349.991 kb on - strand, within Pf6N2E2_2859 at 3349.991 kb on - strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.043 kb on - strand, within Pf6N2E2_2859 at 3350.080 kb on - strand, within Pf6N2E2_2859 at 3350.082 kb on + strand, within Pf6N2E2_2859 at 3350.157 kb on + strand, within Pf6N2E2_2859 at 3350.157 kb on + strand, within Pf6N2E2_2859 at 3350.158 kb on - strand, within Pf6N2E2_2859 at 3350.199 kb on + strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 3,347,754 + +0.2 3,347,754 + -0.5 3,347,754 + -0.7 3,347,754 + +0.4 3,347,755 - +2.5 3,347,755 - +0.8 3,347,755 - +2.1 3,347,755 - +0.0 3,347,758 + -0.2 3,347,758 + -0.3 3,347,874 - Pf6N2E2_2855 0.32 +1.2 3,347,874 - Pf6N2E2_2855 0.32 +0.3 3,347,874 - Pf6N2E2_2855 0.32 -0.1 3,347,874 - Pf6N2E2_2855 0.32 +0.6 3,348,023 + Pf6N2E2_2855 0.75 +0.9 3,348,084 + +0.3 3,348,084 + +1.3 3,348,084 + -0.9 3,348,085 - +0.3 3,348,085 - +0.8 3,348,085 - -0.4 3,348,085 - -1.9 3,348,086 + -0.5 3,348,086 + -1.0 3,348,087 - +0.4 3,348,087 - +2.7 3,348,087 - +0.5 3,348,087 - -0.2 3,348,087 - -0.1 3,348,087 - -0.2 3,348,087 - +2.4 3,348,087 - -0.3 3,348,087 - +0.7 3,348,097 + +0.4 3,348,097 + +1.5 3,348,097 + +0.2 3,348,097 + -0.9 3,348,097 + +0.7 3,348,097 + +0.1 3,348,097 + -0.2 3,348,098 - +1.1 3,348,098 - +0.7 3,348,098 - -0.5 3,348,098 - -0.1 3,348,098 - -2.2 3,348,098 - +0.3 3,348,098 - +0.3 3,348,098 - +0.9 3,348,098 - -0.2 3,348,098 - +0.4 3,348,098 - +0.0 3,348,098 - +0.1 3,348,098 - -1.1 3,348,098 - -0.8 3,348,098 - +0.8 3,348,098 - -0.5 3,348,101 + -0.4 3,348,101 + -0.4 3,348,101 + -0.4 3,348,101 + -0.2 3,348,101 + +0.1 3,348,101 + -0.5 3,348,102 - +0.8 3,348,102 - +0.2 3,348,102 - -0.0 3,348,102 - -1.4 3,348,137 + -1.9 3,348,137 + -0.0 3,348,137 + +0.8 3,348,138 - +0.5 3,348,138 - -0.2 3,348,138 - +0.4 3,348,138 - -0.0 3,348,146 + +0.1 3,348,146 + -1.0 3,348,146 + -1.3 3,348,147 - -0.7 3,348,185 + -0.7 3,348,185 + +1.1 3,348,185 + -1.2 3,348,186 - -1.7 3,348,186 - +0.8 3,348,337 - Pf6N2E2_2856 0.37 -0.0 3,348,337 - Pf6N2E2_2856 0.37 +0.2 3,348,513 + Pf6N2E2_2856 0.77 -0.1 3,348,514 - Pf6N2E2_2856 0.78 -0.6 3,348,514 - Pf6N2E2_2856 0.78 +0.6 3,348,514 - Pf6N2E2_2856 0.78 +1.4 3,348,514 - Pf6N2E2_2856 0.78 -0.5 3,348,514 - Pf6N2E2_2856 0.78 -0.4 3,348,561 + Pf6N2E2_2856 0.89 -0.8 3,348,562 - Pf6N2E2_2856 0.89 +0.5 3,348,604 + +0.6 3,348,722 + +0.3 3,348,722 + +0.6 3,348,722 + +0.1 3,348,722 + +0.1 3,348,722 + +1.5 3,348,723 - -0.7 3,349,042 + Pf6N2E2_2857 0.60 -0.8 3,349,153 + Pf6N2E2_2857 0.83 +0.3 3,349,153 + Pf6N2E2_2857 0.83 -2.7 3,349,154 - Pf6N2E2_2857 0.83 -0.3 3,349,154 - Pf6N2E2_2857 0.83 -1.3 3,349,190 - -2.0 3,349,190 - +0.1 3,349,252 - +1.3 3,349,305 + +0.5 3,349,330 + Pf6N2E2_2858 0.10 -0.8 3,349,330 + Pf6N2E2_2858 0.10 +0.5 3,349,362 + Pf6N2E2_2858 0.15 -0.3 3,349,363 - Pf6N2E2_2858 0.15 -0.6 3,349,363 - Pf6N2E2_2858 0.15 +0.5 3,349,428 - Pf6N2E2_2858 0.26 -0.2 3,349,833 + +0.7 3,349,833 + -1.1 3,349,834 - -1.4 3,349,834 - +2.1 3,349,873 - +1.8 3,349,873 - -0.7 3,349,959 + Pf6N2E2_2859 0.14 -1.1 3,349,990 + Pf6N2E2_2859 0.21 -0.4 3,349,991 - Pf6N2E2_2859 0.21 -1.7 3,349,991 - Pf6N2E2_2859 0.21 +0.3 3,349,991 - Pf6N2E2_2859 0.21 -0.1 3,350,025 + Pf6N2E2_2859 0.28 -0.8 3,350,025 + Pf6N2E2_2859 0.28 -1.2 3,350,025 + Pf6N2E2_2859 0.28 -0.5 3,350,025 + Pf6N2E2_2859 0.28 +0.7 3,350,026 - Pf6N2E2_2859 0.28 -2.4 3,350,026 - Pf6N2E2_2859 0.28 +0.2 3,350,026 - Pf6N2E2_2859 0.28 +0.3 3,350,026 - Pf6N2E2_2859 0.28 +0.2 3,350,026 - Pf6N2E2_2859 0.28 -0.9 3,350,026 - Pf6N2E2_2859 0.28 -0.5 3,350,043 - Pf6N2E2_2859 0.31 +1.1 3,350,080 - Pf6N2E2_2859 0.39 -0.5 3,350,082 + Pf6N2E2_2859 0.39 +0.1 3,350,157 + Pf6N2E2_2859 0.55 +0.1 3,350,157 + Pf6N2E2_2859 0.55 +0.2 3,350,158 - Pf6N2E2_2859 0.55 +0.4 3,350,199 + Pf6N2E2_2859 0.63 +1.1 3,350,200 - Pf6N2E2_2859 0.64 +0.3 3,350,200 - Pf6N2E2_2859 0.64 +0.9 3,350,200 - Pf6N2E2_2859 0.64 +0.1 3,350,200 - Pf6N2E2_2859 0.64 -0.4
Or see this region's nucleotide sequence