Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2410

Experiment: Nickel (II) chloride 1.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2409 and Pf6N2E2_2410 are separated by 33 nucleotidesPf6N2E2_2410 and Pf6N2E2_2411 are separated by 134 nucleotides Pf6N2E2_2409 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 2,860,579 to 2,862,195 _2409 Pf6N2E2_2410 - Citronellyl-CoA dehydrogenase, at 2,862,229 to 2,863,386 _2410 Pf6N2E2_2411 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 2,863,521 to 2,865,491 _2411 Position (kb) 2862 2863 2864Strain fitness (log2 ratio) -2 -1 0 1 2at 2861.314 kb on + strand, within Pf6N2E2_2409at 2861.314 kb on + strand, within Pf6N2E2_2409at 2861.315 kb on - strand, within Pf6N2E2_2409at 2861.429 kb on - strand, within Pf6N2E2_2409at 2861.444 kb on + strand, within Pf6N2E2_2409at 2861.444 kb on + strand, within Pf6N2E2_2409at 2861.479 kb on + strand, within Pf6N2E2_2409at 2861.480 kb on - strand, within Pf6N2E2_2409at 2861.480 kb on - strand, within Pf6N2E2_2409at 2861.480 kb on - strand, within Pf6N2E2_2409at 2861.842 kb on + strand, within Pf6N2E2_2409at 2861.842 kb on + strand, within Pf6N2E2_2409at 2861.842 kb on + strand, within Pf6N2E2_2409at 2862.056 kb on - strandat 2862.121 kb on + strandat 2862.301 kb on + strandat 2862.302 kb on - strandat 2862.302 kb on - strandat 2862.421 kb on + strand, within Pf6N2E2_2410at 2862.422 kb on - strand, within Pf6N2E2_2410at 2862.422 kb on - strand, within Pf6N2E2_2410at 2862.422 kb on - strand, within Pf6N2E2_2410at 2862.422 kb on - strand, within Pf6N2E2_2410at 2862.427 kb on + strand, within Pf6N2E2_2410at 2862.427 kb on + strand, within Pf6N2E2_2410at 2862.428 kb on - strand, within Pf6N2E2_2410at 2862.670 kb on + strand, within Pf6N2E2_2410at 2862.670 kb on + strand, within Pf6N2E2_2410at 2862.670 kb on + strand, within Pf6N2E2_2410at 2862.671 kb on - strand, within Pf6N2E2_2410at 2862.671 kb on - strand, within Pf6N2E2_2410at 2862.671 kb on - strand, within Pf6N2E2_2410at 2862.671 kb on - strand, within Pf6N2E2_2410at 2862.671 kb on - strand, within Pf6N2E2_2410at 2862.741 kb on + strand, within Pf6N2E2_2410at 2862.742 kb on - strand, within Pf6N2E2_2410at 2862.742 kb on - strand, within Pf6N2E2_2410at 2862.742 kb on - strand, within Pf6N2E2_2410at 2863.018 kb on + strand, within Pf6N2E2_2410at 2863.019 kb on - strand, within Pf6N2E2_2410at 2863.144 kb on + strand, within Pf6N2E2_2410at 2863.145 kb on - strand, within Pf6N2E2_2410at 2863.145 kb on - strand, within Pf6N2E2_2410at 2863.145 kb on - strand, within Pf6N2E2_2410at 2863.145 kb on - strand, within Pf6N2E2_2410at 2863.237 kb on + strand, within Pf6N2E2_2410at 2863.490 kb on - strandat 2863.704 kb on + strandat 2863.704 kb on + strandat 2863.705 kb on - strandat 2863.705 kb on - strandat 2864.328 kb on + strand, within Pf6N2E2_2411at 2864.328 kb on + strand, within Pf6N2E2_2411at 2864.328 kb on + strand, within Pf6N2E2_2411at 2864.328 kb on + strand, within Pf6N2E2_2411at 2864.328 kb on + strand, within Pf6N2E2_2411at 2864.379 kb on + strand, within Pf6N2E2_2411at 2864.379 kb on + strand, within Pf6N2E2_2411

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM
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2,861,314 + Pf6N2E2_2409 0.45 +0.3
2,861,314 + Pf6N2E2_2409 0.45 -1.2
2,861,315 - Pf6N2E2_2409 0.46 -0.9
2,861,429 - Pf6N2E2_2409 0.53 -1.7
2,861,444 + Pf6N2E2_2409 0.53 -0.8
2,861,444 + Pf6N2E2_2409 0.53 -1.1
2,861,479 + Pf6N2E2_2409 0.56 -2.5
2,861,480 - Pf6N2E2_2409 0.56 +0.3
2,861,480 - Pf6N2E2_2409 0.56 -0.8
2,861,480 - Pf6N2E2_2409 0.56 +2.3
2,861,842 + Pf6N2E2_2409 0.78 +0.1
2,861,842 + Pf6N2E2_2409 0.78 +0.4
2,861,842 + Pf6N2E2_2409 0.78 -0.4
2,862,056 - -0.9
2,862,121 + +1.4
2,862,301 + +0.3
2,862,302 - +1.4
2,862,302 - -0.1
2,862,421 + Pf6N2E2_2410 0.17 +2.0
2,862,422 - Pf6N2E2_2410 0.17 -0.0
2,862,422 - Pf6N2E2_2410 0.17 -0.2
2,862,422 - Pf6N2E2_2410 0.17 -0.4
2,862,422 - Pf6N2E2_2410 0.17 -0.3
2,862,427 + Pf6N2E2_2410 0.17 -1.1
2,862,427 + Pf6N2E2_2410 0.17 -0.2
2,862,428 - Pf6N2E2_2410 0.17 -0.3
2,862,670 + Pf6N2E2_2410 0.38 -0.6
2,862,670 + Pf6N2E2_2410 0.38 -1.6
2,862,670 + Pf6N2E2_2410 0.38 -1.0
2,862,671 - Pf6N2E2_2410 0.38 -0.7
2,862,671 - Pf6N2E2_2410 0.38 -0.8
2,862,671 - Pf6N2E2_2410 0.38 -1.0
2,862,671 - Pf6N2E2_2410 0.38 -0.2
2,862,671 - Pf6N2E2_2410 0.38 -0.6
2,862,741 + Pf6N2E2_2410 0.44 +0.2
2,862,742 - Pf6N2E2_2410 0.44 +0.6
2,862,742 - Pf6N2E2_2410 0.44 +0.2
2,862,742 - Pf6N2E2_2410 0.44 -0.2
2,863,018 + Pf6N2E2_2410 0.68 +0.9
2,863,019 - Pf6N2E2_2410 0.68 -1.3
2,863,144 + Pf6N2E2_2410 0.79 -0.4
2,863,145 - Pf6N2E2_2410 0.79 -0.7
2,863,145 - Pf6N2E2_2410 0.79 -0.2
2,863,145 - Pf6N2E2_2410 0.79 +1.0
2,863,145 - Pf6N2E2_2410 0.79 -1.2
2,863,237 + Pf6N2E2_2410 0.87 -0.3
2,863,490 - -2.6
2,863,704 + +0.7
2,863,704 + -0.3
2,863,705 - +1.0
2,863,705 - -0.4
2,864,328 + Pf6N2E2_2411 0.41 -0.5
2,864,328 + Pf6N2E2_2411 0.41 -1.2
2,864,328 + Pf6N2E2_2411 0.41 -1.6
2,864,328 + Pf6N2E2_2411 0.41 +1.0
2,864,328 + Pf6N2E2_2411 0.41 -1.0
2,864,379 + Pf6N2E2_2411 0.44 -0.7
2,864,379 + Pf6N2E2_2411 0.44 +0.4

Or see this region's nucleotide sequence