Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1899 and Pf6N2E2_1900 are separated by 361 nucleotides Pf6N2E2_1900 and Pf6N2E2_1901 are separated by 105 nucleotides
Pf6N2E2_1899 - Dienelactone hydrolase and related enzymes, at 2,250,537 to 2,251,511
_1899
Pf6N2E2_1900 - hypothetical protein, at 2,251,873 to 2,252,337
_1900
Pf6N2E2_1901 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140), at 2,252,443 to 2,253,186
_1901
Position (kb)
2250
2251
2252 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2249.560 kb on + strand at 2249.561 kb on - strand at 2249.632 kb on + strand at 2249.632 kb on + strand at 2249.633 kb on - strand at 2249.633 kb on - strand at 2249.634 kb on + strand at 2249.634 kb on + strand at 2249.634 kb on + strand at 2249.634 kb on + strand at 2249.635 kb on - strand at 2249.635 kb on - strand at 2249.635 kb on - strand at 2249.635 kb on - strand at 2249.635 kb on - strand at 2249.635 kb on - strand at 2249.635 kb on - strand at 2249.660 kb on + strand at 2249.688 kb on + strand at 2249.699 kb on + strand at 2249.702 kb on - strand at 2249.702 kb on - strand at 2249.774 kb on - strand at 2249.796 kb on + strand at 2249.796 kb on + strand at 2249.891 kb on - strand at 2249.955 kb on + strand at 2249.956 kb on - strand at 2250.058 kb on - strand at 2250.077 kb on + strand at 2250.077 kb on + strand at 2250.077 kb on + strand at 2250.077 kb on + strand at 2250.078 kb on - strand at 2250.078 kb on - strand at 2250.162 kb on + strand at 2250.162 kb on + strand at 2250.163 kb on - strand at 2250.163 kb on - strand at 2250.164 kb on + strand at 2250.164 kb on + strand at 2250.164 kb on + strand at 2250.164 kb on + strand at 2250.164 kb on + strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.165 kb on - strand at 2250.196 kb on + strand at 2250.196 kb on + strand at 2250.197 kb on - strand at 2250.197 kb on - strand at 2250.197 kb on - strand at 2250.207 kb on - strand at 2250.245 kb on + strand at 2250.245 kb on + strand at 2250.245 kb on + strand at 2250.246 kb on - strand at 2250.246 kb on - strand at 2250.246 kb on - strand at 2250.246 kb on - strand at 2250.279 kb on - strand at 2250.319 kb on + strand at 2250.335 kb on + strand at 2250.335 kb on + strand at 2250.335 kb on + strand at 2250.335 kb on + strand at 2250.335 kb on + strand at 2250.336 kb on - strand at 2250.380 kb on + strand at 2250.380 kb on + strand at 2250.380 kb on + strand at 2250.380 kb on + strand at 2250.380 kb on + strand at 2250.380 kb on + strand at 2250.381 kb on - strand at 2250.381 kb on - strand at 2250.381 kb on - strand at 2250.381 kb on - strand at 2250.385 kb on + strand at 2250.385 kb on + strand at 2250.385 kb on + strand at 2250.385 kb on + strand at 2250.385 kb on + strand at 2250.385 kb on + strand at 2250.385 kb on + strand at 2250.385 kb on + strand at 2250.385 kb on + strand at 2250.386 kb on - strand at 2250.433 kb on - strand at 2250.451 kb on - strand at 2250.459 kb on + strand at 2250.519 kb on + strand at 2250.519 kb on + strand at 2250.519 kb on + strand at 2250.520 kb on - strand at 2250.520 kb on - strand at 2250.520 kb on - strand at 2250.520 kb on - strand at 2250.520 kb on - strand at 2250.525 kb on + strand at 2250.525 kb on + strand at 2250.525 kb on + strand at 2250.525 kb on + strand at 2250.525 kb on + strand at 2250.525 kb on + strand at 2250.593 kb on + strand at 2250.593 kb on + strand at 2250.593 kb on + strand at 2250.593 kb on + strand at 2250.645 kb on + strand, within Pf6N2E2_1899 at 2250.680 kb on + strand, within Pf6N2E2_1899 at 2250.680 kb on + strand, within Pf6N2E2_1899 at 2250.680 kb on + strand, within Pf6N2E2_1899 at 2250.680 kb on + strand, within Pf6N2E2_1899 at 2250.680 kb on + strand, within Pf6N2E2_1899 at 2250.680 kb on + strand, within Pf6N2E2_1899 at 2250.681 kb on - strand, within Pf6N2E2_1899 at 2250.681 kb on - strand, within Pf6N2E2_1899 at 2250.681 kb on - strand, within Pf6N2E2_1899 at 2250.681 kb on - strand, within Pf6N2E2_1899 at 2250.732 kb on + strand, within Pf6N2E2_1899 at 2250.732 kb on + strand, within Pf6N2E2_1899 at 2250.786 kb on + strand, within Pf6N2E2_1899 at 2250.786 kb on + strand, within Pf6N2E2_1899 at 2250.786 kb on + strand, within Pf6N2E2_1899 at 2250.787 kb on - strand, within Pf6N2E2_1899 at 2250.851 kb on - strand, within Pf6N2E2_1899 at 2250.851 kb on - strand, within Pf6N2E2_1899 at 2250.996 kb on + strand, within Pf6N2E2_1899 at 2250.996 kb on + strand, within Pf6N2E2_1899 at 2250.997 kb on - strand, within Pf6N2E2_1899 at 2250.997 kb on - strand, within Pf6N2E2_1899 at 2251.026 kb on + strand, within Pf6N2E2_1899 at 2251.131 kb on + strand, within Pf6N2E2_1899 at 2251.132 kb on - strand, within Pf6N2E2_1899 at 2251.186 kb on - strand, within Pf6N2E2_1899 at 2251.247 kb on + strand, within Pf6N2E2_1899 at 2251.248 kb on - strand, within Pf6N2E2_1899 at 2251.284 kb on + strand, within Pf6N2E2_1899 at 2251.285 kb on - strand, within Pf6N2E2_1899 at 2251.285 kb on - strand, within Pf6N2E2_1899 at 2251.285 kb on - strand, within Pf6N2E2_1899 at 2251.347 kb on + strand, within Pf6N2E2_1899 at 2251.348 kb on - strand, within Pf6N2E2_1899 at 2251.579 kb on - strand at 2251.583 kb on + strand at 2251.645 kb on + strand at 2251.645 kb on + strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.646 kb on - strand at 2251.668 kb on - strand at 2251.676 kb on - strand at 2251.981 kb on - strand, within Pf6N2E2_1900 at 2252.190 kb on + strand, within Pf6N2E2_1900 at 2252.190 kb on + strand, within Pf6N2E2_1900 at 2252.191 kb on - strand, within Pf6N2E2_1900 at 2252.191 kb on - strand, within Pf6N2E2_1900 at 2252.191 kb on - strand, within Pf6N2E2_1900 at 2252.224 kb on + strand, within Pf6N2E2_1900 at 2252.224 kb on + strand, within Pf6N2E2_1900 at 2252.225 kb on - strand, within Pf6N2E2_1900 at 2252.245 kb on + strand, within Pf6N2E2_1900 at 2252.246 kb on - strand, within Pf6N2E2_1900 at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.251 kb on + strand at 2252.251 kb on + strand, within Pf6N2E2_1900 at 2252.252 kb on - strand, within Pf6N2E2_1900 at 2252.356 kb on + strand at 2252.356 kb on + strand at 2252.356 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand at 2252.459 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 2,249,560 + +0.0 2,249,561 - +0.7 2,249,632 + +0.7 2,249,632 + +0.2 2,249,633 - -0.6 2,249,633 - +0.2 2,249,634 + -0.1 2,249,634 + -0.1 2,249,634 + +0.7 2,249,634 + -1.4 2,249,635 - -1.1 2,249,635 - +0.7 2,249,635 - -0.8 2,249,635 - +1.0 2,249,635 - +0.7 2,249,635 - +0.7 2,249,635 - -0.3 2,249,660 + +0.3 2,249,688 + -1.3 2,249,699 + +1.1 2,249,702 - +0.7 2,249,702 - +0.0 2,249,774 - +0.7 2,249,796 + -1.3 2,249,796 + -0.8 2,249,891 - -0.1 2,249,955 + +0.8 2,249,956 - +0.0 2,250,058 - -0.1 2,250,077 + -0.3 2,250,077 + -1.6 2,250,077 + -0.3 2,250,077 + +0.7 2,250,078 - -0.1 2,250,078 - +1.3 2,250,162 + -0.3 2,250,162 + +0.5 2,250,163 - -1.3 2,250,163 - +0.5 2,250,164 + -0.7 2,250,164 + +0.2 2,250,164 + +1.1 2,250,164 + +0.7 2,250,164 + +0.2 2,250,165 - +1.2 2,250,165 - +0.6 2,250,165 - -0.3 2,250,165 - -0.3 2,250,165 - -0.6 2,250,165 - +0.3 2,250,165 - +1.6 2,250,165 - -1.3 2,250,165 - +1.0 2,250,165 - +0.9 2,250,165 - -1.3 2,250,196 + -1.2 2,250,196 + -0.3 2,250,197 - -0.3 2,250,197 - +0.7 2,250,197 - +1.2 2,250,207 - -1.0 2,250,245 + +1.2 2,250,245 + +0.2 2,250,245 + -0.3 2,250,246 - +3.7 2,250,246 - +0.2 2,250,246 - -0.1 2,250,246 - -1.0 2,250,279 - -0.8 2,250,319 + -0.8 2,250,335 + +2.7 2,250,335 + +0.1 2,250,335 + -0.6 2,250,335 + -0.6 2,250,335 + -0.6 2,250,336 - -1.3 2,250,380 + +0.2 2,250,380 + -0.6 2,250,380 + -0.5 2,250,380 + -1.2 2,250,380 + +0.6 2,250,380 + +0.0 2,250,381 - -1.3 2,250,381 - -1.6 2,250,381 - +0.7 2,250,381 - -0.3 2,250,385 + +0.2 2,250,385 + +0.0 2,250,385 + +1.7 2,250,385 + -0.6 2,250,385 + -0.3 2,250,385 + -2.1 2,250,385 + -0.1 2,250,385 + -0.3 2,250,385 + -0.8 2,250,386 - -0.3 2,250,433 - +0.2 2,250,451 - -1.3 2,250,459 + +0.7 2,250,519 + -0.1 2,250,519 + +0.5 2,250,519 + +0.4 2,250,520 - +0.7 2,250,520 - -0.4 2,250,520 - +0.4 2,250,520 - +0.3 2,250,520 - -1.3 2,250,525 + +0.6 2,250,525 + -0.8 2,250,525 + +1.3 2,250,525 + -0.7 2,250,525 + -0.6 2,250,525 + -0.8 2,250,593 + -0.5 2,250,593 + -0.3 2,250,593 + +0.9 2,250,593 + +0.7 2,250,645 + Pf6N2E2_1899 0.11 +0.9 2,250,680 + Pf6N2E2_1899 0.15 -0.7 2,250,680 + Pf6N2E2_1899 0.15 +0.0 2,250,680 + Pf6N2E2_1899 0.15 +0.0 2,250,680 + Pf6N2E2_1899 0.15 +0.3 2,250,680 + Pf6N2E2_1899 0.15 +0.5 2,250,680 + Pf6N2E2_1899 0.15 -0.3 2,250,681 - Pf6N2E2_1899 0.15 -0.7 2,250,681 - Pf6N2E2_1899 0.15 +0.4 2,250,681 - Pf6N2E2_1899 0.15 -0.6 2,250,681 - Pf6N2E2_1899 0.15 -2.1 2,250,732 + Pf6N2E2_1899 0.20 -0.9 2,250,732 + Pf6N2E2_1899 0.20 -1.1 2,250,786 + Pf6N2E2_1899 0.26 +1.3 2,250,786 + Pf6N2E2_1899 0.26 +2.7 2,250,786 + Pf6N2E2_1899 0.26 -0.5 2,250,787 - Pf6N2E2_1899 0.26 +0.3 2,250,851 - Pf6N2E2_1899 0.32 -1.0 2,250,851 - Pf6N2E2_1899 0.32 +1.6 2,250,996 + Pf6N2E2_1899 0.47 +0.3 2,250,996 + Pf6N2E2_1899 0.47 +0.3 2,250,997 - Pf6N2E2_1899 0.47 -1.4 2,250,997 - Pf6N2E2_1899 0.47 -0.8 2,251,026 + Pf6N2E2_1899 0.50 +0.7 2,251,131 + Pf6N2E2_1899 0.61 -0.1 2,251,132 - Pf6N2E2_1899 0.61 +0.7 2,251,186 - Pf6N2E2_1899 0.67 +0.2 2,251,247 + Pf6N2E2_1899 0.73 -0.3 2,251,248 - Pf6N2E2_1899 0.73 -1.2 2,251,284 + Pf6N2E2_1899 0.77 -0.6 2,251,285 - Pf6N2E2_1899 0.77 -0.3 2,251,285 - Pf6N2E2_1899 0.77 -1.6 2,251,285 - Pf6N2E2_1899 0.77 -1.6 2,251,347 + Pf6N2E2_1899 0.83 +0.7 2,251,348 - Pf6N2E2_1899 0.83 -0.8 2,251,579 - -0.1 2,251,583 + +1.5 2,251,645 + -0.3 2,251,645 + +0.7 2,251,646 - +0.2 2,251,646 - -2.7 2,251,646 - -0.0 2,251,646 - -0.8 2,251,646 - +0.0 2,251,646 - -0.7 2,251,668 - -0.3 2,251,676 - +0.3 2,251,981 - Pf6N2E2_1900 0.23 +0.3 2,252,190 + Pf6N2E2_1900 0.68 -0.8 2,252,190 + Pf6N2E2_1900 0.68 +0.2 2,252,191 - Pf6N2E2_1900 0.68 +0.3 2,252,191 - Pf6N2E2_1900 0.68 +0.6 2,252,191 - Pf6N2E2_1900 0.68 +0.4 2,252,224 + Pf6N2E2_1900 0.75 +0.0 2,252,224 + Pf6N2E2_1900 0.75 -1.6 2,252,225 - Pf6N2E2_1900 0.76 +0.7 2,252,245 + Pf6N2E2_1900 0.80 +0.7 2,252,246 - Pf6N2E2_1900 0.80 -0.7 2,252,251 + Pf6N2E2_1900 0.81 -0.7 2,252,251 + Pf6N2E2_1900 0.81 -0.5 2,252,251 + Pf6N2E2_1900 0.81 -1.4 2,252,251 + Pf6N2E2_1900 0.81 -2.4 2,252,251 + +0.7 2,252,251 + Pf6N2E2_1900 0.81 -2.0 2,252,252 - Pf6N2E2_1900 0.82 +0.3 2,252,356 + +0.3 2,252,356 + +0.3 2,252,356 + +0.7 2,252,459 + +1.7 2,252,459 + -0.7 2,252,459 + +0.0 2,252,459 + +0.7 2,252,459 + +0.6 2,252,459 + -0.0
Or see this region's nucleotide sequence