Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1459 and Pf6N2E2_1460 are separated by 2 nucleotides Pf6N2E2_1460 and Pf6N2E2_1461 are separated by 472 nucleotides
Pf6N2E2_1459 - Glycine cleavage system transcriptional activator GcvA, at 1,707,865 to 1,708,770
_1459
Pf6N2E2_1460 - Epoxide hydrolase (EC 3.3.2.9), at 1,708,773 to 1,709,918
_1460
Pf6N2E2_1461 - hypothetical protein, at 1,710,391 to 1,710,672
_1461
Position (kb)
1708
1709
1710 Strain fitness (log2 ratio)
-2
-1
0
1 at 1707.845 kb on + strand at 1707.845 kb on + strand at 1707.846 kb on - strand at 1707.854 kb on - strand at 1707.986 kb on + strand, within Pf6N2E2_1459 at 1707.986 kb on + strand, within Pf6N2E2_1459 at 1707.987 kb on - strand, within Pf6N2E2_1459 at 1707.987 kb on - strand, within Pf6N2E2_1459 at 1708.292 kb on + strand, within Pf6N2E2_1459 at 1708.293 kb on - strand, within Pf6N2E2_1459 at 1708.293 kb on - strand, within Pf6N2E2_1459 at 1708.293 kb on - strand, within Pf6N2E2_1459 at 1708.361 kb on + strand, within Pf6N2E2_1459 at 1708.361 kb on + strand, within Pf6N2E2_1459 at 1708.361 kb on + strand, within Pf6N2E2_1459 at 1708.361 kb on + strand, within Pf6N2E2_1459 at 1708.362 kb on - strand, within Pf6N2E2_1459 at 1708.362 kb on - strand, within Pf6N2E2_1459 at 1708.362 kb on - strand, within Pf6N2E2_1459 at 1708.672 kb on + strand, within Pf6N2E2_1459 at 1708.673 kb on - strand, within Pf6N2E2_1459 at 1708.769 kb on - strand at 1708.907 kb on - strand, within Pf6N2E2_1460 at 1709.019 kb on - strand, within Pf6N2E2_1460 at 1709.229 kb on + strand, within Pf6N2E2_1460 at 1709.229 kb on + strand, within Pf6N2E2_1460 at 1709.275 kb on - strand, within Pf6N2E2_1460 at 1709.275 kb on - strand, within Pf6N2E2_1460 at 1709.373 kb on + strand, within Pf6N2E2_1460 at 1709.374 kb on - strand, within Pf6N2E2_1460 at 1709.640 kb on + strand, within Pf6N2E2_1460 at 1709.679 kb on + strand, within Pf6N2E2_1460 at 1709.680 kb on - strand, within Pf6N2E2_1460 at 1710.143 kb on + strand at 1710.143 kb on + strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.146 kb on - strand at 1710.146 kb on - strand at 1710.146 kb on - strand at 1710.205 kb on + strand at 1710.206 kb on - strand at 1710.206 kb on - strand at 1710.206 kb on - strand at 1710.252 kb on - strand at 1710.338 kb on + strand at 1710.339 kb on - strand at 1710.383 kb on - strand at 1710.383 kb on - strand at 1710.383 kb on - strand at 1710.391 kb on - strand at 1710.391 kb on - strand at 1710.391 kb on - strand at 1710.533 kb on - strand, within Pf6N2E2_1461 at 1710.662 kb on + strand at 1710.769 kb on + strand at 1710.828 kb on + strand at 1710.828 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 1,707,845 + +1.2 1,707,845 + +0.6 1,707,846 - -0.7 1,707,854 - +0.4 1,707,986 + Pf6N2E2_1459 0.13 -1.0 1,707,986 + Pf6N2E2_1459 0.13 -0.4 1,707,987 - Pf6N2E2_1459 0.13 -0.2 1,707,987 - Pf6N2E2_1459 0.13 -0.1 1,708,292 + Pf6N2E2_1459 0.47 -0.4 1,708,293 - Pf6N2E2_1459 0.47 +0.2 1,708,293 - Pf6N2E2_1459 0.47 -0.2 1,708,293 - Pf6N2E2_1459 0.47 -0.1 1,708,361 + Pf6N2E2_1459 0.55 +1.4 1,708,361 + Pf6N2E2_1459 0.55 -2.0 1,708,361 + Pf6N2E2_1459 0.55 +1.5 1,708,361 + Pf6N2E2_1459 0.55 -0.2 1,708,362 - Pf6N2E2_1459 0.55 +0.1 1,708,362 - Pf6N2E2_1459 0.55 +0.0 1,708,362 - Pf6N2E2_1459 0.55 +0.4 1,708,672 + Pf6N2E2_1459 0.89 -0.4 1,708,673 - Pf6N2E2_1459 0.89 -0.4 1,708,769 - -1.5 1,708,907 - Pf6N2E2_1460 0.12 -0.9 1,709,019 - Pf6N2E2_1460 0.21 -2.2 1,709,229 + Pf6N2E2_1460 0.40 +0.6 1,709,229 + Pf6N2E2_1460 0.40 +0.0 1,709,275 - Pf6N2E2_1460 0.44 +0.6 1,709,275 - Pf6N2E2_1460 0.44 -1.4 1,709,373 + Pf6N2E2_1460 0.52 -0.4 1,709,374 - Pf6N2E2_1460 0.52 -1.2 1,709,640 + Pf6N2E2_1460 0.76 +0.4 1,709,679 + Pf6N2E2_1460 0.79 +0.6 1,709,680 - Pf6N2E2_1460 0.79 -1.0 1,710,143 + -1.0 1,710,143 + +0.1 1,710,144 - -0.4 1,710,144 - +0.6 1,710,144 - +1.2 1,710,144 - -2.2 1,710,144 - +0.2 1,710,144 - -0.8 1,710,144 - +0.6 1,710,144 - +0.3 1,710,144 - -1.3 1,710,145 + -0.3 1,710,145 + -0.5 1,710,145 + +0.6 1,710,145 + -0.8 1,710,145 + +1.0 1,710,145 + -0.7 1,710,146 - +0.2 1,710,146 - +0.2 1,710,146 - -1.0 1,710,205 + +0.6 1,710,206 - -0.6 1,710,206 - -1.0 1,710,206 - -0.8 1,710,252 - -0.4 1,710,338 + +0.6 1,710,339 - +0.9 1,710,383 - -1.0 1,710,383 - -2.5 1,710,383 - -0.1 1,710,391 - -0.8 1,710,391 - +0.3 1,710,391 - +0.6 1,710,533 - Pf6N2E2_1461 0.50 -0.4 1,710,662 + +0.6 1,710,769 + +0.9 1,710,828 + +0.8 1,710,828 + -0.1
Or see this region's nucleotide sequence