Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1235 and Pf6N2E2_1236 are separated by 341 nucleotides Pf6N2E2_1236 and Pf6N2E2_1237 are separated by 132 nucleotides
Pf6N2E2_1235 - Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog, at 1,447,206 to 1,448,663
_1235
Pf6N2E2_1236 - Transcriptional regulator, LysR family, at 1,449,005 to 1,449,901
_1236
Pf6N2E2_1237 - Putative transport protein, at 1,450,034 to 1,451,497
_1237
Position (kb)
1449
1450 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1448.017 kb on + strand, within Pf6N2E2_1235 at 1448.017 kb on + strand, within Pf6N2E2_1235 at 1448.017 kb on + strand, within Pf6N2E2_1235 at 1448.017 kb on + strand, within Pf6N2E2_1235 at 1448.044 kb on + strand, within Pf6N2E2_1235 at 1448.107 kb on + strand, within Pf6N2E2_1235 at 1448.107 kb on + strand, within Pf6N2E2_1235 at 1448.107 kb on + strand, within Pf6N2E2_1235 at 1448.108 kb on - strand, within Pf6N2E2_1235 at 1448.140 kb on + strand, within Pf6N2E2_1235 at 1448.140 kb on + strand, within Pf6N2E2_1235 at 1448.140 kb on + strand, within Pf6N2E2_1235 at 1448.140 kb on + strand, within Pf6N2E2_1235 at 1448.158 kb on + strand at 1448.200 kb on + strand, within Pf6N2E2_1235 at 1448.236 kb on + strand, within Pf6N2E2_1235 at 1448.236 kb on + strand, within Pf6N2E2_1235 at 1448.236 kb on + strand, within Pf6N2E2_1235 at 1448.236 kb on + strand, within Pf6N2E2_1235 at 1448.237 kb on - strand, within Pf6N2E2_1235 at 1448.237 kb on - strand, within Pf6N2E2_1235 at 1448.237 kb on - strand, within Pf6N2E2_1235 at 1448.237 kb on - strand, within Pf6N2E2_1235 at 1448.419 kb on + strand, within Pf6N2E2_1235 at 1448.419 kb on + strand, within Pf6N2E2_1235 at 1448.458 kb on + strand, within Pf6N2E2_1235 at 1448.507 kb on - strand, within Pf6N2E2_1235 at 1448.565 kb on - strand at 1448.565 kb on - strand at 1448.664 kb on - strand at 1448.732 kb on - strand at 1448.840 kb on - strand at 1448.856 kb on - strand at 1448.856 kb on - strand at 1448.856 kb on - strand at 1448.868 kb on - strand at 1448.868 kb on - strand at 1448.925 kb on - strand at 1448.928 kb on + strand at 1448.929 kb on - strand at 1448.995 kb on + strand at 1449.163 kb on - strand, within Pf6N2E2_1236 at 1449.425 kb on + strand, within Pf6N2E2_1236 at 1449.425 kb on + strand, within Pf6N2E2_1236 at 1449.426 kb on - strand, within Pf6N2E2_1236 at 1449.941 kb on + strand at 1449.942 kb on - strand at 1450.236 kb on - strand, within Pf6N2E2_1237 at 1450.236 kb on - strand, within Pf6N2E2_1237 at 1450.347 kb on + strand, within Pf6N2E2_1237 at 1450.488 kb on + strand, within Pf6N2E2_1237 at 1450.489 kb on - strand, within Pf6N2E2_1237 at 1450.622 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.800 kb on + strand, within Pf6N2E2_1237 at 1450.873 kb on - strand, within Pf6N2E2_1237
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 1,448,017 + Pf6N2E2_1235 0.56 +0.6 1,448,017 + Pf6N2E2_1235 0.56 +0.5 1,448,017 + Pf6N2E2_1235 0.56 -1.4 1,448,017 + Pf6N2E2_1235 0.56 -0.4 1,448,044 + Pf6N2E2_1235 0.57 +1.9 1,448,107 + Pf6N2E2_1235 0.62 +0.9 1,448,107 + Pf6N2E2_1235 0.62 -0.4 1,448,107 + Pf6N2E2_1235 0.62 +0.7 1,448,108 - Pf6N2E2_1235 0.62 +0.6 1,448,140 + Pf6N2E2_1235 0.64 -0.7 1,448,140 + Pf6N2E2_1235 0.64 -0.0 1,448,140 + Pf6N2E2_1235 0.64 -1.2 1,448,140 + Pf6N2E2_1235 0.64 +1.2 1,448,158 + +0.6 1,448,200 + Pf6N2E2_1235 0.68 -0.9 1,448,236 + Pf6N2E2_1235 0.71 -0.4 1,448,236 + Pf6N2E2_1235 0.71 +1.3 1,448,236 + Pf6N2E2_1235 0.71 -1.4 1,448,236 + Pf6N2E2_1235 0.71 +0.2 1,448,237 - Pf6N2E2_1235 0.71 +0.6 1,448,237 - Pf6N2E2_1235 0.71 -0.4 1,448,237 - Pf6N2E2_1235 0.71 +1.2 1,448,237 - Pf6N2E2_1235 0.71 +0.6 1,448,419 + Pf6N2E2_1235 0.83 +0.6 1,448,419 + Pf6N2E2_1235 0.83 -0.4 1,448,458 + Pf6N2E2_1235 0.86 +0.6 1,448,507 - Pf6N2E2_1235 0.89 -1.6 1,448,565 - +0.1 1,448,565 - -0.4 1,448,664 - +0.6 1,448,732 - -1.2 1,448,840 - -1.0 1,448,856 - -0.0 1,448,856 - -0.8 1,448,856 - -0.2 1,448,868 - +0.3 1,448,868 - -0.4 1,448,925 - +0.8 1,448,928 + +0.6 1,448,929 - -0.4 1,448,995 + +0.6 1,449,163 - Pf6N2E2_1236 0.18 -0.7 1,449,425 + Pf6N2E2_1236 0.47 -0.8 1,449,425 + Pf6N2E2_1236 0.47 -0.7 1,449,426 - Pf6N2E2_1236 0.47 -0.0 1,449,941 + -0.6 1,449,942 - -0.4 1,450,236 - Pf6N2E2_1237 0.14 -0.0 1,450,236 - Pf6N2E2_1237 0.14 -0.4 1,450,347 + Pf6N2E2_1237 0.21 -0.4 1,450,488 + Pf6N2E2_1237 0.31 -0.6 1,450,489 - Pf6N2E2_1237 0.31 +1.3 1,450,622 + Pf6N2E2_1237 0.40 -0.4 1,450,715 + Pf6N2E2_1237 0.47 +1.0 1,450,715 + Pf6N2E2_1237 0.47 -0.0 1,450,715 + Pf6N2E2_1237 0.47 -3.0 1,450,715 + Pf6N2E2_1237 0.47 +0.6 1,450,715 + Pf6N2E2_1237 0.47 -0.3 1,450,715 + Pf6N2E2_1237 0.47 +0.9 1,450,716 - Pf6N2E2_1237 0.47 +2.6 1,450,716 - Pf6N2E2_1237 0.47 -0.2 1,450,716 - Pf6N2E2_1237 0.47 +2.4 1,450,716 - Pf6N2E2_1237 0.47 +0.3 1,450,716 - Pf6N2E2_1237 0.47 -1.0 1,450,716 - Pf6N2E2_1237 0.47 -0.3 1,450,800 + Pf6N2E2_1237 0.52 -1.0 1,450,873 - Pf6N2E2_1237 0.57 -1.3
Or see this region's nucleotide sequence