Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1150 and Pf6N2E2_1151 are separated by 177 nucleotides Pf6N2E2_1151 and Pf6N2E2_1152 are separated by 94 nucleotides Pf6N2E2_1152 and Pf6N2E2_1153 are separated by 3 nucleotides
Pf6N2E2_1150 - CBS-domain-containing membrane protein, at 1,347,827 to 1,348,534
_1150
Pf6N2E2_1151 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 1,348,712 to 1,350,091
_1151
Pf6N2E2_1152 - hypothetical protein, at 1,350,186 to 1,350,920
_1152
Pf6N2E2_1153 - small molecule metabolism; degradation; fatty acid, at 1,350,924 to 1,351,739
_1153
Position (kb)
1348
1349
1350
1351 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1347.738 kb on + strand at 1347.739 kb on - strand at 1347.807 kb on + strand at 1347.807 kb on + strand at 1347.808 kb on - strand at 1347.811 kb on + strand at 1347.811 kb on + strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1348.063 kb on + strand, within Pf6N2E2_1150 at 1348.063 kb on + strand, within Pf6N2E2_1150 at 1348.065 kb on + strand, within Pf6N2E2_1150 at 1348.065 kb on + strand, within Pf6N2E2_1150 at 1348.065 kb on + strand, within Pf6N2E2_1150 at 1348.141 kb on + strand, within Pf6N2E2_1150 at 1348.268 kb on + strand, within Pf6N2E2_1150 at 1348.268 kb on + strand, within Pf6N2E2_1150 at 1348.269 kb on - strand, within Pf6N2E2_1150 at 1348.269 kb on - strand, within Pf6N2E2_1150 at 1348.269 kb on - strand, within Pf6N2E2_1150 at 1348.669 kb on + strand at 1348.670 kb on - strand at 1348.670 kb on - strand at 1348.670 kb on - strand at 1348.791 kb on + strand at 1348.961 kb on - strand, within Pf6N2E2_1151 at 1349.163 kb on + strand, within Pf6N2E2_1151 at 1349.163 kb on + strand, within Pf6N2E2_1151 at 1349.164 kb on - strand, within Pf6N2E2_1151 at 1349.322 kb on + strand, within Pf6N2E2_1151 at 1349.323 kb on - strand, within Pf6N2E2_1151 at 1349.422 kb on + strand, within Pf6N2E2_1151 at 1349.460 kb on + strand, within Pf6N2E2_1151 at 1349.460 kb on + strand, within Pf6N2E2_1151 at 1349.460 kb on + strand, within Pf6N2E2_1151 at 1349.460 kb on + strand, within Pf6N2E2_1151 at 1349.576 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1350.060 kb on - strand at 1350.060 kb on - strand at 1350.060 kb on - strand at 1350.064 kb on - strand at 1350.064 kb on - strand at 1350.166 kb on + strand at 1350.191 kb on + strand at 1350.192 kb on - strand at 1350.230 kb on - strand at 1350.230 kb on - strand at 1350.406 kb on + strand, within Pf6N2E2_1152 at 1350.407 kb on - strand, within Pf6N2E2_1152 at 1350.667 kb on + strand, within Pf6N2E2_1152 at 1350.709 kb on + strand, within Pf6N2E2_1152 at 1350.709 kb on + strand, within Pf6N2E2_1152 at 1350.710 kb on - strand, within Pf6N2E2_1152 at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.872 kb on - strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1350.925 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 1,347,738 + -1.7 1,347,739 - -1.2 1,347,807 + -0.7 1,347,807 + +0.3 1,347,808 - +0.9 1,347,811 + -0.9 1,347,811 + +0.6 1,347,812 - +1.0 1,347,812 - -0.4 1,347,812 - +1.0 1,347,812 - -2.2 1,347,812 - +1.4 1,347,812 - -0.1 1,348,063 + Pf6N2E2_1150 0.33 +0.6 1,348,063 + Pf6N2E2_1150 0.33 +0.9 1,348,065 + Pf6N2E2_1150 0.34 +1.1 1,348,065 + Pf6N2E2_1150 0.34 +0.1 1,348,065 + Pf6N2E2_1150 0.34 -2.2 1,348,141 + Pf6N2E2_1150 0.44 -0.5 1,348,268 + Pf6N2E2_1150 0.62 +1.0 1,348,268 + Pf6N2E2_1150 0.62 +0.3 1,348,269 - Pf6N2E2_1150 0.62 -0.2 1,348,269 - Pf6N2E2_1150 0.62 -0.4 1,348,269 - Pf6N2E2_1150 0.62 +0.2 1,348,669 + -1.0 1,348,670 - +2.1 1,348,670 - +0.1 1,348,670 - +0.9 1,348,791 + -1.2 1,348,961 - Pf6N2E2_1151 0.18 -1.7 1,349,163 + Pf6N2E2_1151 0.33 +1.3 1,349,163 + Pf6N2E2_1151 0.33 +0.2 1,349,164 - Pf6N2E2_1151 0.33 -0.8 1,349,322 + Pf6N2E2_1151 0.44 -1.4 1,349,323 - Pf6N2E2_1151 0.44 -1.4 1,349,422 + Pf6N2E2_1151 0.51 +0.0 1,349,460 + Pf6N2E2_1151 0.54 -1.4 1,349,460 + Pf6N2E2_1151 0.54 -0.6 1,349,460 + Pf6N2E2_1151 0.54 +0.3 1,349,460 + Pf6N2E2_1151 0.54 -1.4 1,349,576 + Pf6N2E2_1151 0.63 -1.7 1,349,952 + Pf6N2E2_1151 0.90 -1.0 1,349,952 + Pf6N2E2_1151 0.90 -1.7 1,349,952 + Pf6N2E2_1151 0.90 +1.0 1,350,060 - +0.6 1,350,060 - +1.3 1,350,060 - +2.1 1,350,064 - +0.1 1,350,064 - -1.1 1,350,166 + +1.0 1,350,191 + -1.7 1,350,192 - +0.3 1,350,230 - +0.9 1,350,230 - -1.0 1,350,406 + Pf6N2E2_1152 0.30 -0.2 1,350,407 - Pf6N2E2_1152 0.30 +0.3 1,350,667 + Pf6N2E2_1152 0.65 +1.0 1,350,709 + Pf6N2E2_1152 0.71 +0.4 1,350,709 + Pf6N2E2_1152 0.71 -0.4 1,350,710 - Pf6N2E2_1152 0.71 +1.2 1,350,871 + -1.9 1,350,871 + -0.4 1,350,871 + +1.6 1,350,871 + -0.5 1,350,872 - +0.6 1,350,925 + +0.3 1,350,925 + +0.6 1,350,925 + -0.5 1,350,925 + -0.5
Or see this region's nucleotide sequence