Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS21615 and HSERO_RS21620 are separated by 141 nucleotides HSERO_RS21620 and HSERO_RS21625 are separated by 78 nucleotides HSERO_RS21625 and HSERO_RS21630 overlap by 4 nucleotides HSERO_RS21630 and HSERO_RS21635 are separated by 21 nucleotides
HSERO_RS21615: HSERO_RS21615 - ABC transporter substrate-binding protein, at 4,949,572 to 4,950,387
_RS21615
HSERO_RS21620: HSERO_RS21620 - amino acid ABC transporter permease, at 4,950,529 to 4,951,272
_RS21620
HSERO_RS21625: HSERO_RS21625 - NAD(FAD)-utilizing dehydrogenase, at 4,951,351 to 4,952,649
_RS21625
HSERO_RS21630: HSERO_RS21630 - SlyX family protein, at 4,952,646 to 4,952,855
_RS21630
HSERO_RS21635: HSERO_RS21635 - DNA-directed DNA polymerase, at 4,952,877 to 4,954,031
_RS21635
Position (kb) 4951
4952
4953 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 4950.375 kb on - strand at 4950.558 kb on - strand at 4950.558 kb on - strand at 4950.594 kb on - strand at 4950.722 kb on + strand, within HSERO_RS21620 at 4950.733 kb on + strand, within HSERO_RS21620 at 4950.848 kb on + strand, within HSERO_RS21620 at 4950.971 kb on - strand, within HSERO_RS21620 at 4951.031 kb on - strand, within HSERO_RS21620 at 4951.031 kb on - strand, within HSERO_RS21620 at 4951.130 kb on - strand, within HSERO_RS21620 at 4951.171 kb on - strand, within HSERO_RS21620 at 4951.179 kb on - strand, within HSERO_RS21620 at 4951.224 kb on + strand at 4951.258 kb on + strand at 4951.304 kb on - strand at 4951.328 kb on + strand at 4951.358 kb on + strand at 4951.358 kb on + strand at 4951.366 kb on - strand at 4951.613 kb on + strand, within HSERO_RS21625 at 4951.937 kb on - strand, within HSERO_RS21625 at 4952.046 kb on + strand, within HSERO_RS21625 at 4952.046 kb on + strand, within HSERO_RS21625 at 4952.115 kb on + strand, within HSERO_RS21625 at 4952.139 kb on + strand, within HSERO_RS21625 at 4952.147 kb on - strand, within HSERO_RS21625 at 4952.172 kb on + strand, within HSERO_RS21625 at 4952.216 kb on + strand, within HSERO_RS21625 at 4952.500 kb on + strand, within HSERO_RS21625 at 4952.857 kb on - strand at 4953.177 kb on - strand, within HSERO_RS21635 at 4953.391 kb on + strand, within HSERO_RS21635 at 4953.497 kb on - strand, within HSERO_RS21635 at 4953.532 kb on - strand at 4953.639 kb on + strand, within HSERO_RS21635
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 4,950,375 - +0.3 4,950,558 - -1.0 4,950,558 - +0.6 4,950,594 - +0.4 4,950,722 + HSERO_RS21620 0.26 -0.9 4,950,733 + HSERO_RS21620 0.27 +0.3 4,950,848 + HSERO_RS21620 0.43 +0.6 4,950,971 - HSERO_RS21620 0.59 +1.0 4,951,031 - HSERO_RS21620 0.67 -0.0 4,951,031 - HSERO_RS21620 0.67 +0.4 4,951,130 - HSERO_RS21620 0.81 +0.5 4,951,171 - HSERO_RS21620 0.86 -0.2 4,951,179 - HSERO_RS21620 0.87 +1.8 4,951,224 + +0.6 4,951,258 + -0.5 4,951,304 - +0.1 4,951,328 + -0.8 4,951,358 + -0.4 4,951,358 + +0.5 4,951,366 - -2.0 4,951,613 + HSERO_RS21625 0.20 +1.0 4,951,937 - HSERO_RS21625 0.45 -0.7 4,952,046 + HSERO_RS21625 0.54 -1.8 4,952,046 + HSERO_RS21625 0.54 -0.1 4,952,115 + HSERO_RS21625 0.59 +0.6 4,952,139 + HSERO_RS21625 0.61 -0.2 4,952,147 - HSERO_RS21625 0.61 +8.3 4,952,172 + HSERO_RS21625 0.63 +0.4 4,952,216 + HSERO_RS21625 0.67 +0.4 4,952,500 + HSERO_RS21625 0.88 +0.1 4,952,857 - +0.6 4,953,177 - HSERO_RS21635 0.26 +0.1 4,953,391 + HSERO_RS21635 0.45 -1.2 4,953,497 - HSERO_RS21635 0.54 +0.3 4,953,532 - -0.2 4,953,639 + HSERO_RS21635 0.66 +0.4
Or see this region's nucleotide sequence