Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18275 and HSERO_RS18280 are separated by 7 nucleotides HSERO_RS18280 and HSERO_RS18285 overlap by 4 nucleotides HSERO_RS18285 and HSERO_RS18290 are separated by 256 nucleotides
HSERO_RS18275: HSERO_RS18275 - CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3- dehydrase reductase, at 4,193,951 to 4,195,033
_RS18275
HSERO_RS18280: HSERO_RS18280 - anthranilate 1,2-dioxygenase small subunit, at 4,195,041 to 4,195,541
_RS18280
HSERO_RS18285: HSERO_RS18285 - Rieske (2Fe-2S) protein, at 4,195,538 to 4,196,818
_RS18285
HSERO_RS18290: HSERO_RS18290 - muconate cycloisomerase, at 4,197,075 to 4,198,235
_RS18290
Position (kb) 4195
4196
4197 Strain fitness (log2 ratio) -1
0
1
2 at 4195.123 kb on + strand, within HSERO_RS18280 at 4195.203 kb on + strand, within HSERO_RS18280 at 4195.562 kb on + strand at 4195.640 kb on + strand at 4195.652 kb on + strand at 4195.692 kb on + strand, within HSERO_RS18285 at 4195.783 kb on + strand, within HSERO_RS18285 at 4195.783 kb on + strand, within HSERO_RS18285 at 4196.141 kb on - strand, within HSERO_RS18285 at 4196.225 kb on + strand, within HSERO_RS18285 at 4196.330 kb on + strand, within HSERO_RS18285 at 4196.363 kb on + strand, within HSERO_RS18285 at 4196.575 kb on + strand, within HSERO_RS18285 at 4196.674 kb on - strand, within HSERO_RS18285 at 4196.738 kb on - strand at 4197.244 kb on - strand, within HSERO_RS18290 at 4197.283 kb on + strand, within HSERO_RS18290 at 4197.509 kb on - strand, within HSERO_RS18290 at 4197.617 kb on - strand, within HSERO_RS18290 at 4197.678 kb on + strand, within HSERO_RS18290
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 4,195,123 + HSERO_RS18280 0.16 -0.9 4,195,203 + HSERO_RS18280 0.32 +0.7 4,195,562 + -0.3 4,195,640 + +0.8 4,195,652 + +0.5 4,195,692 + HSERO_RS18285 0.12 +0.3 4,195,783 + HSERO_RS18285 0.19 +2.2 4,195,783 + HSERO_RS18285 0.19 -0.1 4,196,141 - HSERO_RS18285 0.47 +1.3 4,196,225 + HSERO_RS18285 0.54 +0.2 4,196,330 + HSERO_RS18285 0.62 +0.2 4,196,363 + HSERO_RS18285 0.64 +1.0 4,196,575 + HSERO_RS18285 0.81 +2.4 4,196,674 - HSERO_RS18285 0.89 +0.7 4,196,738 - -0.2 4,197,244 - HSERO_RS18290 0.15 -0.7 4,197,283 + HSERO_RS18290 0.18 +0.9 4,197,509 - HSERO_RS18290 0.37 +0.2 4,197,617 - HSERO_RS18290 0.47 -0.7 4,197,678 + HSERO_RS18290 0.52 +0.3
Or see this region's nucleotide sequence