Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14730 and HSERO_RS14735 are separated by 103 nucleotides HSERO_RS14735 and HSERO_RS14740 are separated by 142 nucleotides
HSERO_RS14730: HSERO_RS14730 - alpha-2-macroglobulin, at 3,339,719 to 3,345,625
_RS14730
HSERO_RS14735: HSERO_RS14735 - LysR family transcripitonal regulator, at 3,345,729 to 3,346,610
_RS14735
HSERO_RS14740: HSERO_RS14740 - efflux transmembrane protein, at 3,346,753 to 3,347,940
_RS14740
Position (kb)
3345
3346
3347 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7 at 3344.896 kb on - strand, within HSERO_RS14730 at 3344.912 kb on + strand, within HSERO_RS14730 at 3344.932 kb on + strand, within HSERO_RS14730 at 3344.940 kb on - strand, within HSERO_RS14730 at 3345.098 kb on - strand at 3345.179 kb on - strand at 3345.238 kb on + strand at 3345.301 kb on - strand at 3345.700 kb on + strand at 3345.810 kb on - strand at 3345.896 kb on + strand, within HSERO_RS14735 at 3345.991 kb on - strand, within HSERO_RS14735 at 3346.348 kb on - strand, within HSERO_RS14735 at 3346.355 kb on + strand, within HSERO_RS14735 at 3346.373 kb on - strand, within HSERO_RS14735 at 3346.424 kb on - strand, within HSERO_RS14735 at 3346.436 kb on + strand, within HSERO_RS14735 at 3346.516 kb on + strand, within HSERO_RS14735 at 3346.569 kb on - strand at 3346.744 kb on + strand at 3347.211 kb on - strand, within HSERO_RS14740 at 3347.211 kb on - strand, within HSERO_RS14740 at 3347.211 kb on - strand, within HSERO_RS14740 at 3347.420 kb on + strand, within HSERO_RS14740 at 3347.425 kb on + strand, within HSERO_RS14740 at 3347.428 kb on - strand, within HSERO_RS14740 at 3347.428 kb on - strand, within HSERO_RS14740 at 3347.465 kb on + strand, within HSERO_RS14740 at 3347.551 kb on + strand, within HSERO_RS14740
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 3,344,896 - HSERO_RS14730 0.88 -0.3 3,344,912 + HSERO_RS14730 0.88 -0.8 3,344,932 + HSERO_RS14730 0.88 -0.8 3,344,940 - HSERO_RS14730 0.88 -1.4 3,345,098 - -1.1 3,345,179 - +0.2 3,345,238 + -0.2 3,345,301 - +0.2 3,345,700 + -0.7 3,345,810 - +0.7 3,345,896 + HSERO_RS14735 0.19 -0.1 3,345,991 - HSERO_RS14735 0.30 +7.0 3,346,348 - HSERO_RS14735 0.70 -0.6 3,346,355 + HSERO_RS14735 0.71 -0.1 3,346,373 - HSERO_RS14735 0.73 +0.3 3,346,424 - HSERO_RS14735 0.79 +0.8 3,346,436 + HSERO_RS14735 0.80 +0.1 3,346,516 + HSERO_RS14735 0.89 +0.2 3,346,569 - -1.0 3,346,744 + +0.1 3,347,211 - HSERO_RS14740 0.39 -1.6 3,347,211 - HSERO_RS14740 0.39 +0.7 3,347,211 - HSERO_RS14740 0.39 +0.1 3,347,420 + HSERO_RS14740 0.56 -0.8 3,347,425 + HSERO_RS14740 0.57 +0.4 3,347,428 - HSERO_RS14740 0.57 +0.3 3,347,428 - HSERO_RS14740 0.57 +0.0 3,347,465 + HSERO_RS14740 0.60 +0.4 3,347,551 + HSERO_RS14740 0.67 -0.1
Or see this region's nucleotide sequence