Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05265 and HSERO_RS05270 are separated by 18 nucleotides HSERO_RS05270 and HSERO_RS05275 are separated by 178 nucleotides HSERO_RS05275 and HSERO_RS05280 overlap by 4 nucleotides HSERO_RS05280 and HSERO_RS05285 overlap by 4 nucleotides
HSERO_RS05265: HSERO_RS05265 - L-fucono-1,5-lactonase (from data) , at 1,165,852 to 1,166,721
_RS05265
HSERO_RS05270: HSERO_RS05270 - TetR family transcriptional regulator, at 1,166,740 to 1,167,360
_RS05270
HSERO_RS05275: HSERO_RS05275 - NADH-ubiquinone oxidoreductase subunit 6, at 1,167,539 to 1,168,825
_RS05275
HSERO_RS05280: HSERO_RS05280 - hypothetical protein, at 1,168,822 to 1,169,673
_RS05280
HSERO_RS05285: HSERO_RS05285 - cyclopropane-fatty-acyl-phospholipid synthase, at 1,169,670 to 1,170,881
_RS05285
Position (kb)
1167
1168
1169 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8 at 1166.565 kb on + strand, within HSERO_RS05265 at 1166.679 kb on + strand at 1166.685 kb on - strand at 1166.687 kb on - strand at 1166.710 kb on + strand at 1166.713 kb on - strand at 1166.715 kb on + strand at 1166.718 kb on - strand at 1166.738 kb on + strand at 1167.533 kb on + strand at 1167.559 kb on - strand at 1167.573 kb on + strand at 1167.672 kb on - strand, within HSERO_RS05275 at 1167.692 kb on + strand, within HSERO_RS05275 at 1167.741 kb on - strand, within HSERO_RS05275 at 1167.744 kb on + strand, within HSERO_RS05275 at 1167.763 kb on + strand, within HSERO_RS05275 at 1167.780 kb on - strand, within HSERO_RS05275 at 1168.002 kb on - strand, within HSERO_RS05275 at 1168.070 kb on - strand, within HSERO_RS05275 at 1168.070 kb on - strand, within HSERO_RS05275 at 1168.132 kb on - strand, within HSERO_RS05275 at 1168.234 kb on - strand, within HSERO_RS05275 at 1168.414 kb on + strand, within HSERO_RS05275 at 1168.606 kb on + strand, within HSERO_RS05275 at 1168.630 kb on - strand, within HSERO_RS05275 at 1168.747 kb on - strand at 1168.761 kb on + strand at 1168.827 kb on - strand at 1168.830 kb on + strand at 1169.021 kb on + strand, within HSERO_RS05280 at 1169.021 kb on + strand, within HSERO_RS05280 at 1169.029 kb on - strand, within HSERO_RS05280 at 1169.118 kb on - strand, within HSERO_RS05280 at 1169.159 kb on - strand, within HSERO_RS05280 at 1169.265 kb on - strand, within HSERO_RS05280 at 1169.292 kb on - strand, within HSERO_RS05280 at 1169.688 kb on - strand at 1169.804 kb on - strand, within HSERO_RS05285
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 1,166,565 + HSERO_RS05265 0.82 +0.6 1,166,679 + +1.0 1,166,685 - -0.3 1,166,687 - -0.4 1,166,710 + -0.5 1,166,713 - -1.0 1,166,715 + -0.2 1,166,718 - +0.1 1,166,738 + +0.0 1,167,533 + +0.2 1,167,559 - -2.2 1,167,573 + -0.4 1,167,672 - HSERO_RS05275 0.10 +8.0 1,167,692 + HSERO_RS05275 0.12 -0.1 1,167,741 - HSERO_RS05275 0.16 +0.3 1,167,744 + HSERO_RS05275 0.16 +0.4 1,167,763 + HSERO_RS05275 0.17 +0.8 1,167,780 - HSERO_RS05275 0.19 +0.4 1,168,002 - HSERO_RS05275 0.36 +0.7 1,168,070 - HSERO_RS05275 0.41 +2.0 1,168,070 - HSERO_RS05275 0.41 +0.4 1,168,132 - HSERO_RS05275 0.46 +1.5 1,168,234 - HSERO_RS05275 0.54 -0.3 1,168,414 + HSERO_RS05275 0.68 -0.0 1,168,606 + HSERO_RS05275 0.83 -0.6 1,168,630 - HSERO_RS05275 0.85 -0.1 1,168,747 - +0.0 1,168,761 + +0.6 1,168,827 - +0.3 1,168,830 + +0.3 1,169,021 + HSERO_RS05280 0.23 +0.6 1,169,021 + HSERO_RS05280 0.23 +1.1 1,169,029 - HSERO_RS05280 0.24 +3.9 1,169,118 - HSERO_RS05280 0.35 +0.2 1,169,159 - HSERO_RS05280 0.40 -0.3 1,169,265 - HSERO_RS05280 0.52 +0.3 1,169,292 - HSERO_RS05280 0.55 -0.2 1,169,688 - -1.1 1,169,804 - HSERO_RS05285 0.11 +0.1
Or see this region's nucleotide sequence