Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02120 and HSERO_RS02125 are separated by 5 nucleotides HSERO_RS02125 and HSERO_RS02130 are separated by 38 nucleotides HSERO_RS02130 and HSERO_RS02135 are separated by 2 nucleotides HSERO_RS02135 and HSERO_RS02140 are separated by 1 nucleotides
HSERO_RS02120: HSERO_RS02120 - phosphomethylpyrimidine synthase ThiC, at 462,763 to 464,676
_RS02120
HSERO_RS02125: HSERO_RS02125 - thiamine biosynthesis protein ThiS, at 464,682 to 464,882
_RS02125
HSERO_RS02130: HSERO_RS02130 - thiazole synthase, at 464,921 to 465,730
_RS02130
HSERO_RS02135: HSERO_RS02135 - phosphomethylpyrimidine kinase, at 465,733 to 466,599
_RS02135
HSERO_RS02140: HSERO_RS02140 - thiamine-phosphate synthase, at 466,601 to 467,233
_RS02140
Position (kb) 464
465
466 Strain fitness (log2 ratio) -1
0
1
2
3 at 463.945 kb on - strand, within HSERO_RS02120 at 464.053 kb on + strand, within HSERO_RS02120 at 464.114 kb on - strand, within HSERO_RS02120 at 464.116 kb on - strand, within HSERO_RS02120 at 464.161 kb on - strand, within HSERO_RS02120 at 464.250 kb on + strand, within HSERO_RS02120 at 464.280 kb on - strand, within HSERO_RS02120 at 464.281 kb on - strand, within HSERO_RS02120 at 464.291 kb on - strand, within HSERO_RS02120 at 464.389 kb on - strand, within HSERO_RS02120 at 464.399 kb on - strand, within HSERO_RS02120 at 464.399 kb on - strand, within HSERO_RS02120 at 464.399 kb on - strand, within HSERO_RS02120 at 464.417 kb on + strand, within HSERO_RS02120 at 464.503 kb on + strand at 464.508 kb on + strand at 464.532 kb on + strand at 464.552 kb on + strand at 464.560 kb on - strand at 464.622 kb on + strand at 464.627 kb on + strand at 464.739 kb on - strand, within HSERO_RS02125 at 464.741 kb on + strand, within HSERO_RS02125 at 464.749 kb on - strand, within HSERO_RS02125 at 464.752 kb on + strand, within HSERO_RS02125 at 464.834 kb on + strand, within HSERO_RS02125 at 464.834 kb on + strand, within HSERO_RS02125 at 464.849 kb on + strand, within HSERO_RS02125 at 464.959 kb on + strand at 464.960 kb on + strand at 464.968 kb on - strand at 465.016 kb on + strand, within HSERO_RS02130 at 465.163 kb on + strand, within HSERO_RS02130 at 465.255 kb on - strand, within HSERO_RS02130 at 465.286 kb on + strand, within HSERO_RS02130 at 465.311 kb on - strand, within HSERO_RS02130 at 465.393 kb on + strand, within HSERO_RS02130 at 465.448 kb on - strand, within HSERO_RS02130 at 465.584 kb on + strand, within HSERO_RS02130 at 465.821 kb on - strand, within HSERO_RS02135 at 465.869 kb on - strand, within HSERO_RS02135 at 465.885 kb on - strand, within HSERO_RS02135 at 465.895 kb on - strand, within HSERO_RS02135 at 465.925 kb on - strand, within HSERO_RS02135 at 465.953 kb on - strand, within HSERO_RS02135 at 466.006 kb on - strand, within HSERO_RS02135 at 466.197 kb on + strand, within HSERO_RS02135 at 466.263 kb on + strand, within HSERO_RS02135 at 466.474 kb on - strand, within HSERO_RS02135 at 466.485 kb on - strand at 466.487 kb on + strand, within HSERO_RS02135 at 466.567 kb on + strand at 466.682 kb on + strand, within HSERO_RS02140
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 463,945 - HSERO_RS02120 0.62 -1.0 464,053 + HSERO_RS02120 0.67 -0.1 464,114 - HSERO_RS02120 0.71 -0.5 464,116 - HSERO_RS02120 0.71 +0.2 464,161 - HSERO_RS02120 0.73 -0.2 464,250 + HSERO_RS02120 0.78 +0.5 464,280 - HSERO_RS02120 0.79 -0.6 464,281 - HSERO_RS02120 0.79 -0.2 464,291 - HSERO_RS02120 0.80 -0.4 464,389 - HSERO_RS02120 0.85 +0.2 464,399 - HSERO_RS02120 0.85 -0.0 464,399 - HSERO_RS02120 0.85 +0.2 464,399 - HSERO_RS02120 0.85 -0.3 464,417 + HSERO_RS02120 0.86 +0.2 464,503 + +0.0 464,508 + -0.4 464,532 + +0.8 464,552 + +0.1 464,560 - +0.9 464,622 + -0.8 464,627 + +1.1 464,739 - HSERO_RS02125 0.28 -0.4 464,741 + HSERO_RS02125 0.29 -0.3 464,749 - HSERO_RS02125 0.33 +0.0 464,752 + HSERO_RS02125 0.35 -0.1 464,834 + HSERO_RS02125 0.76 +0.3 464,834 + HSERO_RS02125 0.76 +1.0 464,849 + HSERO_RS02125 0.83 +0.5 464,959 + -0.1 464,960 + +0.6 464,968 - +0.5 465,016 + HSERO_RS02130 0.12 +0.1 465,163 + HSERO_RS02130 0.30 +2.9 465,255 - HSERO_RS02130 0.41 +0.0 465,286 + HSERO_RS02130 0.45 -1.2 465,311 - HSERO_RS02130 0.48 -1.4 465,393 + HSERO_RS02130 0.58 +0.9 465,448 - HSERO_RS02130 0.65 +0.7 465,584 + HSERO_RS02130 0.82 +1.3 465,821 - HSERO_RS02135 0.10 +0.2 465,869 - HSERO_RS02135 0.16 -0.8 465,885 - HSERO_RS02135 0.18 -0.0 465,895 - HSERO_RS02135 0.19 +1.5 465,925 - HSERO_RS02135 0.22 +0.4 465,953 - HSERO_RS02135 0.25 +0.1 466,006 - HSERO_RS02135 0.31 +0.2 466,197 + HSERO_RS02135 0.54 +0.2 466,263 + HSERO_RS02135 0.61 +2.4 466,474 - HSERO_RS02135 0.85 -0.5 466,485 - +0.7 466,487 + HSERO_RS02135 0.87 +0.9 466,567 + +0.7 466,682 + HSERO_RS02140 0.13 +0.4
Or see this region's nucleotide sequence