Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01965 and HSERO_RS01970 overlap by 4 nucleotides HSERO_RS01970 and HSERO_RS01975 overlap by 1 nucleotides HSERO_RS01975 and HSERO_RS01980 are separated by 16 nucleotides
HSERO_RS01965: HSERO_RS01965 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 430,556 to 431,938
_RS01965
HSERO_RS01970: HSERO_RS01970 - esterase, at 431,935 to 432,525
_RS01970
HSERO_RS01975: HSERO_RS01975 - 4-hydroxybenzoate synthetase, at 432,525 to 433,130
_RS01975
HSERO_RS01980: HSERO_RS01980 - ribonuclease II, at 433,147 to 435,201
_RS01980
Position (kb) 431
432
433 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7 at 430.952 kb on + strand at 431.061 kb on + strand, within HSERO_RS01965 at 431.143 kb on + strand, within HSERO_RS01965 at 431.254 kb on - strand, within HSERO_RS01965 at 431.300 kb on - strand, within HSERO_RS01965 at 431.466 kb on + strand, within HSERO_RS01965 at 431.551 kb on - strand, within HSERO_RS01965 at 431.664 kb on - strand, within HSERO_RS01965 at 431.664 kb on - strand, within HSERO_RS01965 at 431.671 kb on + strand, within HSERO_RS01965 at 431.932 kb on + strand at 431.973 kb on + strand at 432.252 kb on + strand, within HSERO_RS01970 at 432.296 kb on - strand, within HSERO_RS01970 at 432.543 kb on - strand at 432.543 kb on - strand at 432.543 kb on - strand at 432.548 kb on - strand at 432.656 kb on + strand, within HSERO_RS01975 at 432.925 kb on + strand, within HSERO_RS01975 at 432.933 kb on - strand, within HSERO_RS01975 at 432.933 kb on - strand, within HSERO_RS01975 at 433.033 kb on + strand, within HSERO_RS01975 at 433.040 kb on - strand, within HSERO_RS01975
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 430,952 + +0.3 431,061 + HSERO_RS01965 0.37 +0.1 431,143 + HSERO_RS01965 0.42 -1.5 431,254 - HSERO_RS01965 0.50 -0.2 431,300 - HSERO_RS01965 0.54 +0.0 431,466 + HSERO_RS01965 0.66 +0.0 431,551 - HSERO_RS01965 0.72 -1.9 431,664 - HSERO_RS01965 0.80 -0.2 431,664 - HSERO_RS01965 0.80 +0.1 431,671 + HSERO_RS01965 0.81 -0.9 431,932 + +0.8 431,973 + +1.1 432,252 + HSERO_RS01970 0.54 -0.4 432,296 - HSERO_RS01970 0.61 +7.2 432,543 - -0.1 432,543 - -0.6 432,543 - -0.8 432,548 - -0.5 432,656 + HSERO_RS01975 0.22 -0.2 432,925 + HSERO_RS01975 0.66 +0.1 432,933 - HSERO_RS01975 0.67 -0.3 432,933 - HSERO_RS01975 0.67 +0.5 433,033 + HSERO_RS01975 0.84 +0.6 433,040 - HSERO_RS01975 0.85 +0.5
Or see this region's nucleotide sequence