Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_992 and Pf6N2E2_993 overlap by 4 nucleotides Pf6N2E2_993 and Pf6N2E2_994 are separated by 309 nucleotides
Pf6N2E2_992 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 1,160,666 to 1,161,694
_992
Pf6N2E2_993 - Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1), at 1,161,691 to 1,163,196
_993
Pf6N2E2_994 - NADH-dependent dehydrogenase, at 1,163,506 to 1,164,594
_994
Position (kb)
1161
1162
1163
1164 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1160.702 kb on + strand at 1160.774 kb on + strand, within Pf6N2E2_992 at 1160.809 kb on - strand, within Pf6N2E2_992 at 1161.051 kb on - strand, within Pf6N2E2_992 at 1161.208 kb on - strand, within Pf6N2E2_992 at 1161.208 kb on - strand, within Pf6N2E2_992 at 1161.274 kb on + strand, within Pf6N2E2_992 at 1161.274 kb on + strand, within Pf6N2E2_992 at 1161.324 kb on - strand, within Pf6N2E2_992 at 1161.332 kb on + strand, within Pf6N2E2_992 at 1161.356 kb on + strand, within Pf6N2E2_992 at 1161.357 kb on - strand, within Pf6N2E2_992 at 1161.357 kb on - strand, within Pf6N2E2_992 at 1161.480 kb on + strand, within Pf6N2E2_992 at 1161.551 kb on + strand, within Pf6N2E2_992 at 1161.608 kb on + strand at 1161.852 kb on + strand, within Pf6N2E2_993 at 1161.893 kb on + strand, within Pf6N2E2_993 at 1161.919 kb on + strand, within Pf6N2E2_993 at 1161.919 kb on + strand, within Pf6N2E2_993 at 1162.026 kb on + strand, within Pf6N2E2_993 at 1162.030 kb on + strand, within Pf6N2E2_993 at 1162.030 kb on + strand, within Pf6N2E2_993 at 1162.069 kb on + strand, within Pf6N2E2_993 at 1162.172 kb on - strand, within Pf6N2E2_993 at 1162.202 kb on + strand, within Pf6N2E2_993 at 1162.202 kb on + strand, within Pf6N2E2_993 at 1162.203 kb on - strand, within Pf6N2E2_993 at 1162.242 kb on + strand, within Pf6N2E2_993 at 1162.307 kb on - strand, within Pf6N2E2_993 at 1162.307 kb on - strand, within Pf6N2E2_993 at 1162.342 kb on + strand, within Pf6N2E2_993 at 1162.498 kb on + strand, within Pf6N2E2_993 at 1162.563 kb on + strand, within Pf6N2E2_993 at 1162.563 kb on + strand, within Pf6N2E2_993 at 1162.567 kb on - strand, within Pf6N2E2_993 at 1162.615 kb on + strand, within Pf6N2E2_993 at 1162.750 kb on + strand, within Pf6N2E2_993 at 1162.751 kb on - strand, within Pf6N2E2_993 at 1162.866 kb on - strand, within Pf6N2E2_993 at 1162.956 kb on + strand, within Pf6N2E2_993 at 1162.956 kb on + strand at 1163.064 kb on + strand at 1163.124 kb on + strand at 1163.142 kb on + strand at 1163.151 kb on - strand at 1163.158 kb on - strand at 1163.232 kb on + strand at 1163.232 kb on + strand at 1163.255 kb on + strand at 1163.328 kb on + strand at 1163.351 kb on + strand at 1163.464 kb on + strand at 1163.575 kb on - strand at 1163.629 kb on - strand, within Pf6N2E2_994 at 1163.694 kb on - strand, within Pf6N2E2_994 at 1163.717 kb on - strand, within Pf6N2E2_994 at 1163.717 kb on - strand, within Pf6N2E2_994 at 1163.717 kb on - strand, within Pf6N2E2_994 at 1163.792 kb on + strand, within Pf6N2E2_994 at 1163.792 kb on + strand, within Pf6N2E2_994 at 1163.793 kb on - strand, within Pf6N2E2_994 at 1163.837 kb on + strand, within Pf6N2E2_994 at 1163.837 kb on + strand, within Pf6N2E2_994 at 1163.916 kb on - strand, within Pf6N2E2_994 at 1163.955 kb on + strand, within Pf6N2E2_994 at 1163.956 kb on - strand, within Pf6N2E2_994 at 1164.007 kb on - strand, within Pf6N2E2_994 at 1164.049 kb on + strand, within Pf6N2E2_994 at 1164.049 kb on + strand, within Pf6N2E2_994 at 1164.049 kb on + strand, within Pf6N2E2_994 at 1164.053 kb on + strand, within Pf6N2E2_994 at 1164.054 kb on - strand, within Pf6N2E2_994 at 1164.054 kb on - strand, within Pf6N2E2_994 at 1164.054 kb on - strand, within Pf6N2E2_994 at 1164.142 kb on + strand, within Pf6N2E2_994 at 1164.142 kb on + strand, within Pf6N2E2_994 at 1164.142 kb on + strand, within Pf6N2E2_994
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,160,702 + -0.1 1,160,774 + Pf6N2E2_992 0.10 -0.5 1,160,809 - Pf6N2E2_992 0.14 -0.5 1,161,051 - Pf6N2E2_992 0.37 -0.7 1,161,208 - Pf6N2E2_992 0.53 -1.0 1,161,208 - Pf6N2E2_992 0.53 +1.7 1,161,274 + Pf6N2E2_992 0.59 -0.5 1,161,274 + Pf6N2E2_992 0.59 +0.8 1,161,324 - Pf6N2E2_992 0.64 -0.8 1,161,332 + Pf6N2E2_992 0.65 -2.7 1,161,356 + Pf6N2E2_992 0.67 +0.5 1,161,357 - Pf6N2E2_992 0.67 -0.8 1,161,357 - Pf6N2E2_992 0.67 +0.8 1,161,480 + Pf6N2E2_992 0.79 -0.5 1,161,551 + Pf6N2E2_992 0.86 +0.1 1,161,608 + +0.5 1,161,852 + Pf6N2E2_993 0.11 +1.4 1,161,893 + Pf6N2E2_993 0.13 -0.5 1,161,919 + Pf6N2E2_993 0.15 +0.1 1,161,919 + Pf6N2E2_993 0.15 +0.5 1,162,026 + Pf6N2E2_993 0.22 +1.0 1,162,030 + Pf6N2E2_993 0.23 -0.1 1,162,030 + Pf6N2E2_993 0.23 -2.3 1,162,069 + Pf6N2E2_993 0.25 -0.9 1,162,172 - Pf6N2E2_993 0.32 -1.8 1,162,202 + Pf6N2E2_993 0.34 -0.8 1,162,202 + Pf6N2E2_993 0.34 +1.5 1,162,203 - Pf6N2E2_993 0.34 -0.0 1,162,242 + Pf6N2E2_993 0.37 -1.0 1,162,307 - Pf6N2E2_993 0.41 -1.0 1,162,307 - Pf6N2E2_993 0.41 -1.0 1,162,342 + Pf6N2E2_993 0.43 +0.2 1,162,498 + Pf6N2E2_993 0.54 -1.9 1,162,563 + Pf6N2E2_993 0.58 -1.5 1,162,563 + Pf6N2E2_993 0.58 -0.5 1,162,567 - Pf6N2E2_993 0.58 -0.5 1,162,615 + Pf6N2E2_993 0.61 -1.0 1,162,750 + Pf6N2E2_993 0.70 -0.0 1,162,751 - Pf6N2E2_993 0.70 +0.4 1,162,866 - Pf6N2E2_993 0.78 -2.0 1,162,956 + Pf6N2E2_993 0.84 +2.1 1,162,956 + +1.5 1,163,064 + -2.6 1,163,124 + -0.5 1,163,142 + -1.8 1,163,151 - +0.5 1,163,158 - -0.5 1,163,232 + -1.6 1,163,232 + -0.0 1,163,255 + +0.1 1,163,328 + +0.4 1,163,351 + -0.0 1,163,464 + +0.5 1,163,575 - -0.0 1,163,629 - Pf6N2E2_994 0.11 +0.0 1,163,694 - Pf6N2E2_994 0.17 -0.0 1,163,717 - Pf6N2E2_994 0.19 +1.4 1,163,717 - Pf6N2E2_994 0.19 -2.0 1,163,717 - Pf6N2E2_994 0.19 -1.3 1,163,792 + Pf6N2E2_994 0.26 -0.4 1,163,792 + Pf6N2E2_994 0.26 +0.5 1,163,793 - Pf6N2E2_994 0.26 +1.5 1,163,837 + Pf6N2E2_994 0.30 -0.1 1,163,837 + Pf6N2E2_994 0.30 -0.8 1,163,916 - Pf6N2E2_994 0.38 -1.8 1,163,955 + Pf6N2E2_994 0.41 -1.2 1,163,956 - Pf6N2E2_994 0.41 -0.5 1,164,007 - Pf6N2E2_994 0.46 +0.5 1,164,049 + Pf6N2E2_994 0.50 -0.5 1,164,049 + Pf6N2E2_994 0.50 -1.0 1,164,049 + Pf6N2E2_994 0.50 -0.2 1,164,053 + Pf6N2E2_994 0.50 +0.7 1,164,054 - Pf6N2E2_994 0.50 +0.5 1,164,054 - Pf6N2E2_994 0.50 -0.5 1,164,054 - Pf6N2E2_994 0.50 +1.5 1,164,142 + Pf6N2E2_994 0.58 -2.0 1,164,142 + Pf6N2E2_994 0.58 +0.8 1,164,142 + Pf6N2E2_994 0.58 -0.8
Or see this region's nucleotide sequence