Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_816

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_815 and Pf6N2E2_816 overlap by 1 nucleotidesPf6N2E2_816 and Pf6N2E2_817 overlap by 4 nucleotidesPf6N2E2_817 and Pf6N2E2_818 are separated by 5 nucleotides Pf6N2E2_815 - Signal transduction histidine kinase, at 946,888 to 950,370 _815 Pf6N2E2_816 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 950,370 to 951,185 _816 Pf6N2E2_817 - Protein-glutamate methylesterase (EC 3.1.1.61), at 951,182 to 951,769 _817 Pf6N2E2_818 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 951,775 to 953,055 _818 Position (kb) 950 951 952Strain fitness (log2 ratio) -2 -1 0 1 2at 949.793 kb on - strand, within Pf6N2E2_815at 949.840 kb on + strand, within Pf6N2E2_815at 949.841 kb on - strand, within Pf6N2E2_815at 949.841 kb on - strand, within Pf6N2E2_815at 950.150 kb on + strandat 950.150 kb on + strandat 950.150 kb on + strandat 950.150 kb on + strandat 950.150 kb on + strandat 950.151 kb on - strandat 950.151 kb on - strandat 950.151 kb on - strandat 950.151 kb on - strandat 950.151 kb on - strandat 950.227 kb on - strandat 950.427 kb on + strandat 950.427 kb on + strandat 950.428 kb on - strandat 950.428 kb on - strandat 950.433 kb on + strandat 950.658 kb on + strand, within Pf6N2E2_816at 950.659 kb on - strand, within Pf6N2E2_816at 950.709 kb on + strandat 950.710 kb on - strand, within Pf6N2E2_816at 950.764 kb on - strand, within Pf6N2E2_816at 950.835 kb on - strand, within Pf6N2E2_816at 950.835 kb on - strand, within Pf6N2E2_816at 950.835 kb on - strand, within Pf6N2E2_816at 950.835 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.887 kb on - strand, within Pf6N2E2_816at 950.887 kb on - strand, within Pf6N2E2_816at 950.887 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 951.019 kb on + strandat 951.022 kb on - strand, within Pf6N2E2_816at 951.022 kb on - strand, within Pf6N2E2_816at 951.022 kb on - strand, within Pf6N2E2_816at 951.022 kb on - strand, within Pf6N2E2_816at 951.315 kb on + strand, within Pf6N2E2_817at 951.316 kb on - strand, within Pf6N2E2_817at 951.426 kb on + strand, within Pf6N2E2_817at 951.426 kb on + strand, within Pf6N2E2_817at 951.426 kb on + strand, within Pf6N2E2_817at 951.426 kb on + strand, within Pf6N2E2_817at 951.426 kb on + strand, within Pf6N2E2_817at 951.427 kb on - strand, within Pf6N2E2_817at 951.427 kb on - strand, within Pf6N2E2_817at 951.427 kb on - strand, within Pf6N2E2_817at 951.427 kb on - strand, within Pf6N2E2_817at 951.427 kb on - strand, within Pf6N2E2_817at 951.427 kb on - strand, within Pf6N2E2_817at 951.427 kb on - strand, within Pf6N2E2_817at 951.428 kb on + strand, within Pf6N2E2_817at 951.428 kb on + strand, within Pf6N2E2_817at 951.428 kb on + strand, within Pf6N2E2_817at 951.428 kb on + strand, within Pf6N2E2_817at 951.428 kb on + strand, within Pf6N2E2_817at 951.429 kb on - strand, within Pf6N2E2_817at 951.429 kb on - strand, within Pf6N2E2_817at 951.429 kb on - strand, within Pf6N2E2_817at 951.429 kb on - strand, within Pf6N2E2_817at 951.429 kb on - strand, within Pf6N2E2_817at 951.429 kb on - strand, within Pf6N2E2_817at 951.429 kb on - strand, within Pf6N2E2_817at 951.721 kb on + strandat 951.727 kb on + strandat 951.727 kb on + strandat 951.727 kb on + strandat 951.728 kb on - strandat 951.786 kb on + strandat 951.786 kb on + strandat 951.787 kb on - strandat 951.787 kb on - strandat 951.787 kb on - strandat 952.021 kb on - strand, within Pf6N2E2_818at 952.057 kb on + strand, within Pf6N2E2_818at 952.057 kb on + strand, within Pf6N2E2_818at 952.057 kb on + strand, within Pf6N2E2_818at 952.057 kb on + strand, within Pf6N2E2_818at 952.058 kb on - strand, within Pf6N2E2_818at 952.058 kb on - strand, within Pf6N2E2_818at 952.058 kb on - strand, within Pf6N2E2_818at 952.156 kb on + strand, within Pf6N2E2_818at 952.157 kb on - strand, within Pf6N2E2_818at 952.157 kb on - strand, within Pf6N2E2_818at 952.157 kb on - strand, within Pf6N2E2_818

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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949,793 - Pf6N2E2_815 0.83 +1.6
949,840 + Pf6N2E2_815 0.85 -1.7
949,841 - Pf6N2E2_815 0.85 -0.4
949,841 - Pf6N2E2_815 0.85 -0.7
950,150 + +0.9
950,150 + -0.7
950,150 + +0.2
950,150 + +0.6
950,150 + +2.4
950,151 - +2.2
950,151 - +0.6
950,151 - +0.6
950,151 - +0.6
950,151 - +0.6
950,227 - +1.0
950,427 + +0.6
950,427 + -0.2
950,428 - +0.6
950,428 - +0.9
950,433 + -0.7
950,658 + Pf6N2E2_816 0.35 -1.3
950,659 - Pf6N2E2_816 0.35 +0.6
950,709 + -0.8
950,710 - Pf6N2E2_816 0.42 +0.6
950,764 - Pf6N2E2_816 0.48 -0.4
950,835 - Pf6N2E2_816 0.57 -0.4
950,835 - Pf6N2E2_816 0.57 +0.0
950,835 - Pf6N2E2_816 0.57 +0.6
950,835 - Pf6N2E2_816 0.57 +1.6
950,839 - Pf6N2E2_816 0.57 -0.4
950,839 - Pf6N2E2_816 0.57 +0.0
950,839 - Pf6N2E2_816 0.57 -1.4
950,839 - Pf6N2E2_816 0.57 +1.6
950,839 - Pf6N2E2_816 0.57 +0.0
950,839 - Pf6N2E2_816 0.57 +0.6
950,887 - Pf6N2E2_816 0.63 +1.6
950,887 - Pf6N2E2_816 0.63 -1.4
950,887 - Pf6N2E2_816 0.63 +0.6
950,890 - Pf6N2E2_816 0.64 +0.9
950,890 - Pf6N2E2_816 0.64 -0.4
950,890 - Pf6N2E2_816 0.64 -1.0
950,890 - Pf6N2E2_816 0.64 -0.9
950,890 - Pf6N2E2_816 0.64 +0.0
950,890 - Pf6N2E2_816 0.64 +0.3
950,890 - Pf6N2E2_816 0.64 +0.6
950,890 - Pf6N2E2_816 0.64 +0.6
951,019 + -1.0
951,022 - Pf6N2E2_816 0.80 -2.2
951,022 - Pf6N2E2_816 0.80 -1.4
951,022 - Pf6N2E2_816 0.80 -0.4
951,022 - Pf6N2E2_816 0.80 +0.6
951,315 + Pf6N2E2_817 0.23 -0.5
951,316 - Pf6N2E2_817 0.23 +0.6
951,426 + Pf6N2E2_817 0.41 -0.6
951,426 + Pf6N2E2_817 0.41 -0.2
951,426 + Pf6N2E2_817 0.41 +1.4
951,426 + Pf6N2E2_817 0.41 -2.1
951,426 + Pf6N2E2_817 0.41 -1.5
951,427 - Pf6N2E2_817 0.42 +0.6
951,427 - Pf6N2E2_817 0.42 -1.0
951,427 - Pf6N2E2_817 0.42 +0.6
951,427 - Pf6N2E2_817 0.42 +0.3
951,427 - Pf6N2E2_817 0.42 -0.4
951,427 - Pf6N2E2_817 0.42 -0.4
951,427 - Pf6N2E2_817 0.42 +0.6
951,428 + Pf6N2E2_817 0.42 -1.8
951,428 + Pf6N2E2_817 0.42 -0.7
951,428 + Pf6N2E2_817 0.42 +0.2
951,428 + Pf6N2E2_817 0.42 +0.1
951,428 + Pf6N2E2_817 0.42 -0.6
951,429 - Pf6N2E2_817 0.42 +0.6
951,429 - Pf6N2E2_817 0.42 -0.4
951,429 - Pf6N2E2_817 0.42 +0.6
951,429 - Pf6N2E2_817 0.42 +0.6
951,429 - Pf6N2E2_817 0.42 +0.6
951,429 - Pf6N2E2_817 0.42 +1.9
951,429 - Pf6N2E2_817 0.42 +0.6
951,721 + -0.4
951,727 + -0.9
951,727 + -1.1
951,727 + -0.2
951,728 - -2.0
951,786 + -0.2
951,786 + +0.6
951,787 - -0.1
951,787 - +0.0
951,787 - +1.6
952,021 - Pf6N2E2_818 0.19 +1.6
952,057 + Pf6N2E2_818 0.22 +1.2
952,057 + Pf6N2E2_818 0.22 +0.6
952,057 + Pf6N2E2_818 0.22 +1.6
952,057 + Pf6N2E2_818 0.22 +1.2
952,058 - Pf6N2E2_818 0.22 -0.4
952,058 - Pf6N2E2_818 0.22 -0.4
952,058 - Pf6N2E2_818 0.22 +0.6
952,156 + Pf6N2E2_818 0.30 +0.6
952,157 - Pf6N2E2_818 0.30 +0.6
952,157 - Pf6N2E2_818 0.30 +0.6
952,157 - Pf6N2E2_818 0.30 +0.6

Or see this region's nucleotide sequence