Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5512 and Pf6N2E2_5513 are separated by 228 nucleotides Pf6N2E2_5513 and Pf6N2E2_5514 are separated by 137 nucleotides Pf6N2E2_5514 and Pf6N2E2_5515 are separated by 294 nucleotides
Pf6N2E2_5512 - glycosyl transferase, group 1 family protein, at 6,328,564 to 6,329,655
_5512
Pf6N2E2_5513 - ATPase component BioM of energizing module of biotin ECF transporter, at 6,329,884 to 6,331,485
_5513
Pf6N2E2_5514 - Zn-ribbon-containing, possibly RNA-binding protein and truncated derivatives, at 6,331,623 to 6,332,078
_5514
Pf6N2E2_5515 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 6,332,373 to 6,335,108
_5515
Position (kb)
6329
6330
6331
6332 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 6329.177 kb on + strand, within Pf6N2E2_5512 at 6329.178 kb on - strand, within Pf6N2E2_5512 at 6329.178 kb on - strand, within Pf6N2E2_5512 at 6329.255 kb on + strand, within Pf6N2E2_5512 at 6329.270 kb on + strand, within Pf6N2E2_5512 at 6329.270 kb on + strand, within Pf6N2E2_5512 at 6329.270 kb on + strand, within Pf6N2E2_5512 at 6329.270 kb on + strand, within Pf6N2E2_5512 at 6329.270 kb on + strand, within Pf6N2E2_5512 at 6329.271 kb on - strand, within Pf6N2E2_5512 at 6329.271 kb on - strand, within Pf6N2E2_5512 at 6329.339 kb on + strand, within Pf6N2E2_5512 at 6329.340 kb on - strand, within Pf6N2E2_5512 at 6329.403 kb on - strand, within Pf6N2E2_5512 at 6329.403 kb on - strand, within Pf6N2E2_5512 at 6329.434 kb on + strand, within Pf6N2E2_5512 at 6329.435 kb on - strand, within Pf6N2E2_5512 at 6329.435 kb on - strand, within Pf6N2E2_5512 at 6329.435 kb on - strand, within Pf6N2E2_5512 at 6329.522 kb on + strand, within Pf6N2E2_5512 at 6329.522 kb on + strand, within Pf6N2E2_5512 at 6329.522 kb on + strand, within Pf6N2E2_5512 at 6329.522 kb on + strand, within Pf6N2E2_5512 at 6329.522 kb on + strand, within Pf6N2E2_5512 at 6329.522 kb on + strand, within Pf6N2E2_5512 at 6329.523 kb on - strand, within Pf6N2E2_5512 at 6329.523 kb on - strand, within Pf6N2E2_5512 at 6329.523 kb on - strand, within Pf6N2E2_5512 at 6329.523 kb on - strand, within Pf6N2E2_5512 at 6329.523 kb on - strand, within Pf6N2E2_5512 at 6329.618 kb on + strand at 6329.618 kb on + strand at 6329.618 kb on + strand at 6329.618 kb on + strand at 6329.618 kb on + strand at 6329.619 kb on - strand at 6329.648 kb on + strand at 6329.648 kb on + strand at 6329.659 kb on + strand at 6329.659 kb on + strand at 6329.659 kb on + strand at 6329.659 kb on + strand at 6329.659 kb on + strand at 6329.659 kb on + strand at 6329.659 kb on + strand at 6329.659 kb on + strand at 6329.659 kb on + strand at 6329.659 kb on + strand at 6329.660 kb on - strand at 6329.660 kb on - strand at 6329.660 kb on - strand at 6329.668 kb on + strand at 6329.668 kb on + strand at 6329.670 kb on + strand at 6329.670 kb on + strand at 6329.670 kb on + strand at 6329.670 kb on + strand at 6329.671 kb on - strand at 6329.671 kb on - strand at 6329.671 kb on - strand at 6329.718 kb on + strand at 6329.718 kb on + strand at 6329.719 kb on - strand at 6329.719 kb on - strand at 6329.719 kb on - strand at 6329.721 kb on + strand at 6329.721 kb on + strand at 6329.721 kb on + strand at 6329.721 kb on + strand at 6329.722 kb on - strand at 6329.722 kb on - strand at 6329.727 kb on + strand at 6329.727 kb on + strand at 6329.727 kb on + strand at 6329.727 kb on + strand at 6329.727 kb on + strand at 6329.727 kb on + strand at 6329.727 kb on + strand at 6329.727 kb on + strand at 6329.727 kb on + strand at 6329.727 kb on + strand at 6329.728 kb on - strand at 6329.728 kb on - strand at 6329.728 kb on - strand at 6329.728 kb on - strand at 6329.728 kb on - strand at 6329.744 kb on + strand at 6329.744 kb on + strand at 6329.761 kb on + strand at 6329.765 kb on + strand at 6329.765 kb on + strand at 6329.765 kb on + strand at 6329.765 kb on + strand at 6329.765 kb on + strand at 6329.765 kb on + strand at 6329.765 kb on + strand at 6329.765 kb on + strand at 6329.766 kb on - strand at 6329.928 kb on + strand at 6329.928 kb on + strand at 6329.929 kb on - strand at 6329.929 kb on - strand at 6329.948 kb on + strand at 6329.948 kb on + strand at 6329.948 kb on + strand at 6329.948 kb on + strand at 6329.948 kb on + strand at 6329.948 kb on + strand at 6329.948 kb on + strand at 6329.948 kb on + strand at 6329.948 kb on + strand at 6329.949 kb on - strand at 6329.949 kb on - strand at 6329.949 kb on - strand at 6329.949 kb on - strand at 6329.949 kb on - strand at 6329.949 kb on - strand at 6329.950 kb on + strand at 6329.950 kb on + strand at 6329.950 kb on + strand at 6329.951 kb on - strand at 6329.951 kb on - strand at 6329.951 kb on - strand at 6329.951 kb on - strand at 6329.951 kb on - strand at 6329.952 kb on + strand at 6329.952 kb on + strand at 6329.952 kb on + strand at 6329.952 kb on + strand at 6329.952 kb on + strand at 6329.952 kb on + strand at 6329.953 kb on - strand at 6329.953 kb on - strand at 6329.953 kb on - strand at 6329.953 kb on - strand at 6329.953 kb on - strand at 6329.953 kb on - strand at 6329.953 kb on - strand at 6329.953 kb on - strand at 6329.953 kb on - strand at 6329.953 kb on - strand at 6329.973 kb on + strand at 6329.974 kb on - strand at 6329.974 kb on - strand at 6329.995 kb on + strand at 6329.995 kb on + strand at 6329.995 kb on + strand at 6329.995 kb on + strand at 6329.995 kb on + strand at 6329.996 kb on - strand at 6329.996 kb on - strand at 6329.996 kb on - strand at 6329.996 kb on - strand at 6329.996 kb on - strand at 6329.996 kb on - strand at 6329.996 kb on - strand at 6329.996 kb on - strand at 6330.244 kb on - strand, within Pf6N2E2_5513 at 6330.409 kb on + strand, within Pf6N2E2_5513 at 6330.901 kb on + strand, within Pf6N2E2_5513 at 6330.902 kb on - strand, within Pf6N2E2_5513 at 6331.231 kb on + strand, within Pf6N2E2_5513 at 6331.231 kb on + strand, within Pf6N2E2_5513 at 6331.232 kb on - strand, within Pf6N2E2_5513 at 6331.232 kb on - strand, within Pf6N2E2_5513 at 6331.232 kb on - strand, within Pf6N2E2_5513 at 6331.261 kb on + strand, within Pf6N2E2_5513 at 6331.261 kb on + strand, within Pf6N2E2_5513 at 6331.261 kb on + strand, within Pf6N2E2_5513 at 6331.262 kb on - strand, within Pf6N2E2_5513 at 6331.262 kb on - strand, within Pf6N2E2_5513 at 6331.262 kb on - strand, within Pf6N2E2_5513 at 6331.392 kb on + strand at 6331.392 kb on + strand at 6331.392 kb on + strand at 6331.392 kb on + strand at 6331.392 kb on + strand at 6331.392 kb on + strand at 6331.393 kb on - strand at 6331.393 kb on - strand at 6331.543 kb on + strand at 6332.113 kb on - strand at 6332.166 kb on - strand at 6332.212 kb on + strand at 6332.212 kb on + strand at 6332.351 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 6,329,177 + Pf6N2E2_5512 0.56 +0.5 6,329,178 - Pf6N2E2_5512 0.56 +0.1 6,329,178 - Pf6N2E2_5512 0.56 +1.6 6,329,255 + Pf6N2E2_5512 0.63 +1.2 6,329,270 + Pf6N2E2_5512 0.65 -0.3 6,329,270 + Pf6N2E2_5512 0.65 -0.6 6,329,270 + Pf6N2E2_5512 0.65 +1.8 6,329,270 + Pf6N2E2_5512 0.65 -1.2 6,329,270 + Pf6N2E2_5512 0.65 +1.6 6,329,271 - Pf6N2E2_5512 0.65 -0.3 6,329,271 - Pf6N2E2_5512 0.65 +0.3 6,329,339 + Pf6N2E2_5512 0.71 +0.6 6,329,340 - Pf6N2E2_5512 0.71 -0.3 6,329,403 - Pf6N2E2_5512 0.77 -0.5 6,329,403 - Pf6N2E2_5512 0.77 -0.6 6,329,434 + Pf6N2E2_5512 0.80 +1.3 6,329,435 - Pf6N2E2_5512 0.80 -1.0 6,329,435 - Pf6N2E2_5512 0.80 -0.6 6,329,435 - Pf6N2E2_5512 0.80 +1.4 6,329,522 + Pf6N2E2_5512 0.88 +0.4 6,329,522 + Pf6N2E2_5512 0.88 -0.1 6,329,522 + Pf6N2E2_5512 0.88 +1.4 6,329,522 + Pf6N2E2_5512 0.88 -0.2 6,329,522 + Pf6N2E2_5512 0.88 +0.6 6,329,522 + Pf6N2E2_5512 0.88 -0.2 6,329,523 - Pf6N2E2_5512 0.88 +0.1 6,329,523 - Pf6N2E2_5512 0.88 +0.7 6,329,523 - Pf6N2E2_5512 0.88 +2.6 6,329,523 - Pf6N2E2_5512 0.88 -0.3 6,329,523 - Pf6N2E2_5512 0.88 -0.3 6,329,618 + -0.1 6,329,618 + +0.4 6,329,618 + -0.6 6,329,618 + -2.5 6,329,618 + -0.7 6,329,619 - +1.6 6,329,648 + +1.2 6,329,648 + +0.2 6,329,659 + -1.3 6,329,659 + +0.5 6,329,659 + +1.5 6,329,659 + +0.7 6,329,659 + +0.3 6,329,659 + -0.4 6,329,659 + +0.7 6,329,659 + +0.3 6,329,659 + +1.1 6,329,659 + +1.0 6,329,660 - +0.1 6,329,660 - +0.1 6,329,660 - -0.1 6,329,668 + +0.2 6,329,668 + -1.5 6,329,670 + +0.4 6,329,670 + -0.9 6,329,670 + +0.1 6,329,670 + +0.4 6,329,671 - -0.1 6,329,671 - -0.2 6,329,671 - +0.4 6,329,718 + +0.5 6,329,718 + +0.9 6,329,719 - +1.7 6,329,719 - +0.6 6,329,719 - +1.5 6,329,721 + +0.5 6,329,721 + +0.6 6,329,721 + +0.5 6,329,721 + +0.1 6,329,722 - -0.4 6,329,722 - +3.1 6,329,727 + +2.1 6,329,727 + +1.6 6,329,727 + +0.5 6,329,727 + +0.4 6,329,727 + +1.0 6,329,727 + +0.6 6,329,727 + +0.1 6,329,727 + -0.5 6,329,727 + +0.1 6,329,727 + +0.6 6,329,728 - -0.5 6,329,728 - +0.1 6,329,728 - -0.5 6,329,728 - -0.9 6,329,728 - +1.1 6,329,744 + +0.0 6,329,744 + -0.3 6,329,761 + +0.3 6,329,765 + -0.5 6,329,765 + -0.5 6,329,765 + -1.0 6,329,765 + +0.6 6,329,765 + +0.3 6,329,765 + +0.5 6,329,765 + +3.9 6,329,765 + +0.4 6,329,766 - -0.5 6,329,928 + +0.1 6,329,928 + +0.1 6,329,929 - +0.4 6,329,929 - +1.6 6,329,948 + -0.1 6,329,948 + +0.7 6,329,948 + -1.4 6,329,948 + +0.5 6,329,948 + -0.1 6,329,948 + -0.1 6,329,948 + -0.5 6,329,948 + +0.6 6,329,948 + +0.7 6,329,949 - -1.8 6,329,949 - -2.3 6,329,949 - +1.1 6,329,949 - -0.3 6,329,949 - -0.3 6,329,949 - -0.7 6,329,950 + +1.1 6,329,950 + +0.8 6,329,950 + -0.7 6,329,951 - -0.1 6,329,951 - -0.1 6,329,951 - +0.5 6,329,951 - -0.5 6,329,951 - -0.3 6,329,952 + +0.6 6,329,952 + +0.2 6,329,952 + +0.4 6,329,952 + +0.4 6,329,952 + -0.4 6,329,952 + -1.6 6,329,953 - +0.3 6,329,953 - +0.1 6,329,953 - +0.6 6,329,953 - -0.4 6,329,953 - +0.7 6,329,953 - +1.6 6,329,953 - +0.2 6,329,953 - +0.2 6,329,953 - -0.4 6,329,953 - +0.3 6,329,973 + +1.9 6,329,974 - -0.2 6,329,974 - +0.1 6,329,995 + -0.3 6,329,995 + +1.1 6,329,995 + -0.4 6,329,995 + -0.6 6,329,995 + +0.7 6,329,996 - -1.6 6,329,996 - +0.2 6,329,996 - +1.8 6,329,996 - -0.8 6,329,996 - +1.4 6,329,996 - +0.4 6,329,996 - +0.4 6,329,996 - -0.8 6,330,244 - Pf6N2E2_5513 0.22 +1.3 6,330,409 + Pf6N2E2_5513 0.33 +0.9 6,330,901 + Pf6N2E2_5513 0.63 +1.2 6,330,902 - Pf6N2E2_5513 0.64 +0.3 6,331,231 + Pf6N2E2_5513 0.84 +0.6 6,331,231 + Pf6N2E2_5513 0.84 +0.1 6,331,232 - Pf6N2E2_5513 0.84 +1.5 6,331,232 - Pf6N2E2_5513 0.84 +0.1 6,331,232 - Pf6N2E2_5513 0.84 +0.5 6,331,261 + Pf6N2E2_5513 0.86 +0.5 6,331,261 + Pf6N2E2_5513 0.86 +0.7 6,331,261 + Pf6N2E2_5513 0.86 +1.3 6,331,262 - Pf6N2E2_5513 0.86 -0.0 6,331,262 - Pf6N2E2_5513 0.86 +0.2 6,331,262 - Pf6N2E2_5513 0.86 +0.2 6,331,392 + +2.1 6,331,392 + -0.3 6,331,392 + +1.0 6,331,392 + +0.7 6,331,392 + -0.8 6,331,392 + +0.2 6,331,393 - +0.8 6,331,393 - +0.5 6,331,543 + -0.4 6,332,113 - +0.7 6,332,166 - +1.1 6,332,212 + +0.4 6,332,212 + -0.5 6,332,351 + +0.1
Or see this region's nucleotide sequence