Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4797

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4794 and Pf6N2E2_4795 are separated by 8 nucleotidesPf6N2E2_4795 and Pf6N2E2_4796 are separated by 83 nucleotidesPf6N2E2_4796 and Pf6N2E2_4797 overlap by 10 nucleotidesPf6N2E2_4797 and Pf6N2E2_4798 are separated by 79 nucleotides Pf6N2E2_4794 - TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 5,543,816 to 5,544,841 _4794 Pf6N2E2_4795 - Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY, at 5,544,850 to 5,545,419 _4795 Pf6N2E2_4796 - Dihydroneopterin aldolase (EC 4.1.2.25), at 5,545,503 to 5,545,847 _4796 Pf6N2E2_4797 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 5,545,838 to 5,546,356 _4797 Pf6N2E2_4798 - tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25), at 5,546,436 to 5,547,662 _4798 Position (kb) 5545 5546 5547Strain fitness (log2 ratio) -2 -1 0 1 2at 5544.851 kb on + strandat 5544.886 kb on + strandat 5544.886 kb on + strandat 5544.886 kb on + strandat 5544.887 kb on - strandat 5544.938 kb on - strand, within Pf6N2E2_4795at 5545.025 kb on + strand, within Pf6N2E2_4795at 5545.025 kb on + strand, within Pf6N2E2_4795at 5545.026 kb on - strand, within Pf6N2E2_4795at 5545.133 kb on + strand, within Pf6N2E2_4795at 5545.134 kb on - strand, within Pf6N2E2_4795at 5545.144 kb on + strand, within Pf6N2E2_4795at 5545.144 kb on + strand, within Pf6N2E2_4795at 5545.144 kb on + strand, within Pf6N2E2_4795at 5545.145 kb on - strand, within Pf6N2E2_4795at 5545.145 kb on - strand, within Pf6N2E2_4795at 5545.422 kb on + strandat 5545.422 kb on + strandat 5545.422 kb on + strandat 5545.422 kb on + strandat 5545.422 kb on + strandat 5545.423 kb on - strandat 5545.426 kb on + strandat 5545.452 kb on + strandat 5545.452 kb on + strandat 5546.129 kb on + strand, within Pf6N2E2_4797at 5546.130 kb on - strand, within Pf6N2E2_4797at 5546.335 kb on + strandat 5546.335 kb on + strandat 5546.335 kb on + strandat 5546.335 kb on + strandat 5546.399 kb on - strandat 5546.399 kb on - strandat 5546.454 kb on - strandat 5546.455 kb on + strandat 5546.455 kb on + strandat 5546.455 kb on + strandat 5546.456 kb on - strandat 5546.456 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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5,544,851 + +0.7
5,544,886 + +0.4
5,544,886 + +0.9
5,544,886 + +1.6
5,544,887 - -2.0
5,544,938 - Pf6N2E2_4795 0.15 -1.1
5,545,025 + Pf6N2E2_4795 0.31 +0.9
5,545,025 + Pf6N2E2_4795 0.31 +0.3
5,545,026 - Pf6N2E2_4795 0.31 -0.3
5,545,133 + Pf6N2E2_4795 0.50 +2.0
5,545,134 - Pf6N2E2_4795 0.50 +0.3
5,545,144 + Pf6N2E2_4795 0.52 +0.7
5,545,144 + Pf6N2E2_4795 0.52 +1.3
5,545,144 + Pf6N2E2_4795 0.52 +1.2
5,545,145 - Pf6N2E2_4795 0.52 -2.3
5,545,145 - Pf6N2E2_4795 0.52 -2.1
5,545,422 + +1.0
5,545,422 + +1.0
5,545,422 + +0.7
5,545,422 + +1.8
5,545,422 + +1.2
5,545,423 - +1.3
5,545,426 + +1.1
5,545,452 + +0.8
5,545,452 + +0.8
5,546,129 + Pf6N2E2_4797 0.56 -1.3
5,546,130 - Pf6N2E2_4797 0.56 -1.1
5,546,335 + -1.2
5,546,335 + +0.7
5,546,335 + -1.5
5,546,335 + +0.3
5,546,399 - -0.5
5,546,399 - -0.2
5,546,454 - -1.0
5,546,455 + +0.5
5,546,455 + +0.9
5,546,455 + +1.2
5,546,456 - -0.9
5,546,456 - -0.5

Or see this region's nucleotide sequence