Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4046 and Pf6N2E2_4047 are separated by 84 nucleotides Pf6N2E2_4047 and Pf6N2E2_4048 overlap by 1 nucleotides Pf6N2E2_4048 and Pf6N2E2_4049 are separated by 112 nucleotides
Pf6N2E2_4046 - FIG00953482: hypothetical protein, at 4,726,072 to 4,728,033
_4046
Pf6N2E2_4047 - Tryptophan synthase alpha chain (EC 4.2.1.20), at 4,728,118 to 4,728,930
_4047
Pf6N2E2_4048 - Tryptophan synthase beta chain (EC 4.2.1.20), at 4,728,930 to 4,730,162
_4048
Pf6N2E2_4049 - Glycine cleavage system transcriptional activator, at 4,730,275 to 4,731,192
_4049
Position (kb)
4728
4729
4730
4731 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4728.053 kb on + strand at 4728.054 kb on - strand at 4728.054 kb on - strand at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.408 kb on - strand, within Pf6N2E2_4047 at 4728.408 kb on - strand, within Pf6N2E2_4047 at 4728.408 kb on - strand, within Pf6N2E2_4047 at 4728.409 kb on + strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.627 kb on - strand, within Pf6N2E2_4047 at 4728.627 kb on - strand, within Pf6N2E2_4047 at 4729.157 kb on - strand, within Pf6N2E2_4048 at 4729.157 kb on - strand, within Pf6N2E2_4048 at 4729.294 kb on - strand, within Pf6N2E2_4048 at 4729.294 kb on - strand, within Pf6N2E2_4048 at 4729.571 kb on - strand, within Pf6N2E2_4048 at 4729.571 kb on - strand, within Pf6N2E2_4048 at 4729.571 kb on - strand, within Pf6N2E2_4048 at 4729.571 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.928 kb on - strand, within Pf6N2E2_4048 at 4729.952 kb on - strand, within Pf6N2E2_4048 at 4729.952 kb on - strand, within Pf6N2E2_4048 at 4729.952 kb on - strand, within Pf6N2E2_4048 at 4729.976 kb on - strand, within Pf6N2E2_4048 at 4730.084 kb on - strand at 4730.084 kb on - strand at 4730.084 kb on - strand at 4730.084 kb on - strand at 4730.207 kb on + strand at 4730.207 kb on + strand at 4730.208 kb on - strand at 4730.208 kb on - strand at 4730.343 kb on - strand at 4730.384 kb on + strand, within Pf6N2E2_4049 at 4730.385 kb on - strand, within Pf6N2E2_4049 at 4730.601 kb on + strand, within Pf6N2E2_4049 at 4730.602 kb on - strand, within Pf6N2E2_4049 at 4730.602 kb on - strand, within Pf6N2E2_4049 at 4730.711 kb on + strand, within Pf6N2E2_4049 at 4730.711 kb on + strand, within Pf6N2E2_4049 at 4730.749 kb on + strand, within Pf6N2E2_4049 at 4730.749 kb on + strand, within Pf6N2E2_4049 at 4730.749 kb on + strand, within Pf6N2E2_4049 at 4730.749 kb on + strand, within Pf6N2E2_4049 at 4730.750 kb on - strand, within Pf6N2E2_4049 at 4730.750 kb on - strand, within Pf6N2E2_4049 at 4730.750 kb on - strand, within Pf6N2E2_4049 at 4730.795 kb on - strand, within Pf6N2E2_4049 at 4730.953 kb on + strand, within Pf6N2E2_4049 at 4730.953 kb on + strand, within Pf6N2E2_4049 at 4730.954 kb on - strand, within Pf6N2E2_4049 at 4730.954 kb on - strand, within Pf6N2E2_4049 at 4730.954 kb on - strand, within Pf6N2E2_4049 at 4730.954 kb on - strand, within Pf6N2E2_4049 at 4730.954 kb on - strand, within Pf6N2E2_4049 at 4730.954 kb on - strand, within Pf6N2E2_4049 at 4730.956 kb on + strand, within Pf6N2E2_4049 at 4730.956 kb on + strand, within Pf6N2E2_4049 at 4730.956 kb on + strand, within Pf6N2E2_4049 at 4730.957 kb on - strand, within Pf6N2E2_4049 at 4730.957 kb on - strand, within Pf6N2E2_4049 at 4730.957 kb on - strand, within Pf6N2E2_4049
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 4,728,053 + +0.7 4,728,054 - -1.7 4,728,054 - -0.7 4,728,405 - Pf6N2E2_4047 0.35 +1.5 4,728,405 - Pf6N2E2_4047 0.35 -1.5 4,728,405 - Pf6N2E2_4047 0.35 -0.2 4,728,405 - Pf6N2E2_4047 0.35 +1.1 4,728,405 - Pf6N2E2_4047 0.35 +0.1 4,728,405 - Pf6N2E2_4047 0.35 +0.1 4,728,405 - Pf6N2E2_4047 0.35 -0.5 4,728,405 - Pf6N2E2_4047 0.35 -0.1 4,728,408 - Pf6N2E2_4047 0.36 -2.2 4,728,408 - Pf6N2E2_4047 0.36 -0.8 4,728,408 - Pf6N2E2_4047 0.36 +0.4 4,728,409 + Pf6N2E2_4047 0.36 +0.5 4,728,410 - Pf6N2E2_4047 0.36 -1.1 4,728,410 - Pf6N2E2_4047 0.36 -0.5 4,728,410 - Pf6N2E2_4047 0.36 -0.8 4,728,410 - Pf6N2E2_4047 0.36 -1.6 4,728,410 - Pf6N2E2_4047 0.36 +0.3 4,728,410 - Pf6N2E2_4047 0.36 -0.3 4,728,410 - Pf6N2E2_4047 0.36 -0.3 4,728,410 - Pf6N2E2_4047 0.36 +0.9 4,728,410 - Pf6N2E2_4047 0.36 -2.1 4,728,627 - Pf6N2E2_4047 0.63 +0.2 4,728,627 - Pf6N2E2_4047 0.63 -0.4 4,729,157 - Pf6N2E2_4048 0.18 +1.1 4,729,157 - Pf6N2E2_4048 0.18 -0.3 4,729,294 - Pf6N2E2_4048 0.30 -0.8 4,729,294 - Pf6N2E2_4048 0.30 -1.1 4,729,571 - Pf6N2E2_4048 0.52 -0.6 4,729,571 - Pf6N2E2_4048 0.52 -1.8 4,729,571 - Pf6N2E2_4048 0.52 +1.3 4,729,571 - Pf6N2E2_4048 0.52 +0.5 4,729,730 - Pf6N2E2_4048 0.65 -1.1 4,729,730 - Pf6N2E2_4048 0.65 -0.6 4,729,730 - Pf6N2E2_4048 0.65 +0.2 4,729,730 - Pf6N2E2_4048 0.65 -0.5 4,729,730 - Pf6N2E2_4048 0.65 -0.4 4,729,907 - Pf6N2E2_4048 0.79 -0.6 4,729,907 - Pf6N2E2_4048 0.79 -0.4 4,729,907 - Pf6N2E2_4048 0.79 -0.2 4,729,907 - Pf6N2E2_4048 0.79 -0.1 4,729,907 - Pf6N2E2_4048 0.79 -1.1 4,729,907 - Pf6N2E2_4048 0.79 -0.6 4,729,928 - Pf6N2E2_4048 0.81 -0.7 4,729,952 - Pf6N2E2_4048 0.83 -0.9 4,729,952 - Pf6N2E2_4048 0.83 +0.1 4,729,952 - Pf6N2E2_4048 0.83 -0.8 4,729,976 - Pf6N2E2_4048 0.85 -0.1 4,730,084 - -0.5 4,730,084 - -0.9 4,730,084 - -0.5 4,730,084 - -0.1 4,730,207 + +1.1 4,730,207 + +0.5 4,730,208 - -0.0 4,730,208 - -0.2 4,730,343 - +1.3 4,730,384 + Pf6N2E2_4049 0.12 -0.6 4,730,385 - Pf6N2E2_4049 0.12 +0.5 4,730,601 + Pf6N2E2_4049 0.36 -0.1 4,730,602 - Pf6N2E2_4049 0.36 +1.5 4,730,602 - Pf6N2E2_4049 0.36 -0.9 4,730,711 + Pf6N2E2_4049 0.47 -0.5 4,730,711 + Pf6N2E2_4049 0.47 +0.4 4,730,749 + Pf6N2E2_4049 0.52 -0.1 4,730,749 + Pf6N2E2_4049 0.52 +0.1 4,730,749 + Pf6N2E2_4049 0.52 -0.1 4,730,749 + Pf6N2E2_4049 0.52 +0.4 4,730,750 - Pf6N2E2_4049 0.52 -0.5 4,730,750 - Pf6N2E2_4049 0.52 -0.1 4,730,750 - Pf6N2E2_4049 0.52 -0.1 4,730,795 - Pf6N2E2_4049 0.57 -0.5 4,730,953 + Pf6N2E2_4049 0.74 +0.9 4,730,953 + Pf6N2E2_4049 0.74 -0.1 4,730,954 - Pf6N2E2_4049 0.74 -0.7 4,730,954 - Pf6N2E2_4049 0.74 +0.2 4,730,954 - Pf6N2E2_4049 0.74 -1.1 4,730,954 - Pf6N2E2_4049 0.74 -0.6 4,730,954 - Pf6N2E2_4049 0.74 +1.4 4,730,954 - Pf6N2E2_4049 0.74 +1.0 4,730,956 + Pf6N2E2_4049 0.74 +0.3 4,730,956 + Pf6N2E2_4049 0.74 +0.8 4,730,956 + Pf6N2E2_4049 0.74 +2.5 4,730,957 - Pf6N2E2_4049 0.74 +1.2 4,730,957 - Pf6N2E2_4049 0.74 -0.6 4,730,957 - Pf6N2E2_4049 0.74 +0.6
Or see this region's nucleotide sequence