Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2879 and Pf6N2E2_2880 are separated by 285 nucleotides Pf6N2E2_2880 and Pf6N2E2_2881 are separated by 149 nucleotides Pf6N2E2_2881 and Pf6N2E2_2882 are separated by 17 nucleotides
Pf6N2E2_2879 - membrane protein, putative, at 3,366,303 to 3,368,303
_2879
Pf6N2E2_2880 - FIG00959696: hypothetical protein, at 3,368,589 to 3,369,053
_2880
Pf6N2E2_2881 - Putative cytoplasmic protein, at 3,369,203 to 3,369,496
_2881
Pf6N2E2_2882 - Isopenicillin N epimerase (EC 5.1.1.17), at 3,369,514 to 3,370,701
_2882
Position (kb)
3369
3370 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3368.246 kb on + strand at 3368.294 kb on + strand at 3368.294 kb on + strand at 3368.295 kb on - strand at 3368.295 kb on - strand at 3368.305 kb on + strand at 3368.305 kb on + strand at 3368.305 kb on + strand at 3368.305 kb on + strand at 3368.305 kb on + strand at 3368.306 kb on - strand at 3368.306 kb on - strand at 3368.306 kb on - strand at 3368.306 kb on - strand at 3368.306 kb on - strand at 3368.306 kb on - strand at 3368.306 kb on - strand at 3368.337 kb on + strand at 3368.337 kb on + strand at 3368.337 kb on + strand at 3368.337 kb on + strand at 3368.337 kb on + strand at 3368.337 kb on + strand at 3368.337 kb on + strand at 3368.338 kb on - strand at 3368.338 kb on - strand at 3368.338 kb on - strand at 3368.338 kb on - strand at 3368.338 kb on - strand at 3368.338 kb on - strand at 3368.347 kb on - strand at 3368.374 kb on + strand at 3368.416 kb on + strand at 3368.416 kb on + strand at 3368.422 kb on + strand at 3368.422 kb on + strand at 3368.423 kb on - strand at 3368.590 kb on + strand at 3368.590 kb on + strand at 3368.590 kb on + strand at 3368.590 kb on + strand at 3368.590 kb on + strand at 3368.591 kb on - strand at 3368.609 kb on + strand at 3368.654 kb on - strand, within Pf6N2E2_2880 at 3368.679 kb on - strand, within Pf6N2E2_2880 at 3368.722 kb on + strand, within Pf6N2E2_2880 at 3368.875 kb on - strand, within Pf6N2E2_2880 at 3369.227 kb on - strand at 3369.314 kb on + strand, within Pf6N2E2_2881 at 3369.314 kb on + strand, within Pf6N2E2_2881 at 3369.314 kb on + strand, within Pf6N2E2_2881 at 3369.314 kb on + strand, within Pf6N2E2_2881 at 3369.315 kb on - strand, within Pf6N2E2_2881 at 3369.315 kb on - strand, within Pf6N2E2_2881 at 3369.531 kb on - strand at 3369.575 kb on - strand at 3369.627 kb on + strand at 3369.627 kb on + strand at 3369.627 kb on + strand at 3369.628 kb on - strand at 3369.628 kb on - strand at 3369.628 kb on - strand at 3369.652 kb on + strand, within Pf6N2E2_2882 at 3369.653 kb on - strand, within Pf6N2E2_2882 at 3369.694 kb on + strand, within Pf6N2E2_2882 at 3369.694 kb on + strand, within Pf6N2E2_2882 at 3369.694 kb on + strand, within Pf6N2E2_2882 at 3369.695 kb on - strand, within Pf6N2E2_2882 at 3369.695 kb on - strand, within Pf6N2E2_2882 at 3369.695 kb on - strand, within Pf6N2E2_2882 at 3369.829 kb on + strand, within Pf6N2E2_2882 at 3369.829 kb on + strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.878 kb on - strand, within Pf6N2E2_2882 at 3369.878 kb on - strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.037 kb on - strand, within Pf6N2E2_2882 at 3370.037 kb on - strand, within Pf6N2E2_2882 at 3370.235 kb on - strand, within Pf6N2E2_2882 at 3370.235 kb on - strand, within Pf6N2E2_2882 at 3370.288 kb on + strand, within Pf6N2E2_2882 at 3370.289 kb on - strand, within Pf6N2E2_2882 at 3370.289 kb on - strand, within Pf6N2E2_2882 at 3370.421 kb on - strand, within Pf6N2E2_2882 at 3370.421 kb on - strand, within Pf6N2E2_2882 at 3370.421 kb on - strand, within Pf6N2E2_2882
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,368,246 + +0.7 3,368,294 + +0.3 3,368,294 + +0.1 3,368,295 - -0.5 3,368,295 - +0.1 3,368,305 + +1.7 3,368,305 + -1.4 3,368,305 + +0.7 3,368,305 + +0.6 3,368,305 + +0.5 3,368,306 - +1.1 3,368,306 - -0.9 3,368,306 - +0.9 3,368,306 - +0.2 3,368,306 - +0.5 3,368,306 - -0.2 3,368,306 - -0.8 3,368,337 + -0.7 3,368,337 + +0.0 3,368,337 + -0.5 3,368,337 + -0.2 3,368,337 + -0.2 3,368,337 + +0.9 3,368,337 + +0.5 3,368,338 - -0.5 3,368,338 - -0.0 3,368,338 - +0.1 3,368,338 - -0.8 3,368,338 - +1.3 3,368,338 - -1.0 3,368,347 - -1.0 3,368,374 + +0.5 3,368,416 + +0.3 3,368,416 + +0.4 3,368,422 + -0.3 3,368,422 + -0.0 3,368,423 - +0.2 3,368,590 + +0.2 3,368,590 + +0.4 3,368,590 + -0.3 3,368,590 + +0.1 3,368,590 + +0.4 3,368,591 - -0.9 3,368,609 + +1.1 3,368,654 - Pf6N2E2_2880 0.14 +1.2 3,368,679 - Pf6N2E2_2880 0.19 +0.1 3,368,722 + Pf6N2E2_2880 0.29 +1.5 3,368,875 - Pf6N2E2_2880 0.62 -0.4 3,369,227 - +0.7 3,369,314 + Pf6N2E2_2881 0.38 +2.1 3,369,314 + Pf6N2E2_2881 0.38 -0.7 3,369,314 + Pf6N2E2_2881 0.38 +0.9 3,369,314 + Pf6N2E2_2881 0.38 -0.4 3,369,315 - Pf6N2E2_2881 0.38 -1.1 3,369,315 - Pf6N2E2_2881 0.38 -2.3 3,369,531 - -0.9 3,369,575 - +0.5 3,369,627 + -0.9 3,369,627 + -1.2 3,369,627 + +0.2 3,369,628 - +0.5 3,369,628 - -0.7 3,369,628 - -1.5 3,369,652 + Pf6N2E2_2882 0.12 -0.6 3,369,653 - Pf6N2E2_2882 0.12 +0.2 3,369,694 + Pf6N2E2_2882 0.15 -0.1 3,369,694 + Pf6N2E2_2882 0.15 +1.1 3,369,694 + Pf6N2E2_2882 0.15 -1.3 3,369,695 - Pf6N2E2_2882 0.15 -0.4 3,369,695 - Pf6N2E2_2882 0.15 +1.1 3,369,695 - Pf6N2E2_2882 0.15 +1.5 3,369,829 + Pf6N2E2_2882 0.27 +0.9 3,369,829 + Pf6N2E2_2882 0.27 +0.2 3,369,830 - Pf6N2E2_2882 0.27 +0.7 3,369,830 - Pf6N2E2_2882 0.27 +0.4 3,369,830 - Pf6N2E2_2882 0.27 -0.5 3,369,830 - Pf6N2E2_2882 0.27 +1.0 3,369,830 - Pf6N2E2_2882 0.27 -0.3 3,369,830 - Pf6N2E2_2882 0.27 +0.2 3,369,830 - Pf6N2E2_2882 0.27 +0.7 3,369,878 - Pf6N2E2_2882 0.31 +1.9 3,369,878 - Pf6N2E2_2882 0.31 +0.1 3,370,036 + Pf6N2E2_2882 0.44 -0.5 3,370,036 + Pf6N2E2_2882 0.44 -0.2 3,370,036 + Pf6N2E2_2882 0.44 -0.7 3,370,036 + Pf6N2E2_2882 0.44 +0.2 3,370,037 - Pf6N2E2_2882 0.44 +0.3 3,370,037 - Pf6N2E2_2882 0.44 +0.7 3,370,235 - Pf6N2E2_2882 0.61 -1.0 3,370,235 - Pf6N2E2_2882 0.61 +0.8 3,370,288 + Pf6N2E2_2882 0.65 +0.8 3,370,289 - Pf6N2E2_2882 0.65 +2.7 3,370,289 - Pf6N2E2_2882 0.65 +1.1 3,370,421 - Pf6N2E2_2882 0.76 +1.1 3,370,421 - Pf6N2E2_2882 0.76 -0.5 3,370,421 - Pf6N2E2_2882 0.76 +0.1
Or see this region's nucleotide sequence