Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2833 and Pf6N2E2_2834 are separated by 1 nucleotides Pf6N2E2_2834 and Pf6N2E2_2835 are separated by 37 nucleotides Pf6N2E2_2835 and Pf6N2E2_2836 overlap by 1 nucleotides Pf6N2E2_2836 and Pf6N2E2_2837 overlap by 4 nucleotides
Pf6N2E2_2833 - Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3), at 3,322,926 to 3,323,492
_2833
Pf6N2E2_2834 - Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3), at 3,323,494 to 3,324,198
_2834
Pf6N2E2_2835 - Beta-ketoadipyl CoA thiolase (EC 2.3.1.-), at 3,324,236 to 3,325,438
_2835
Pf6N2E2_2836 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6); Glutaconate CoA-transferase subunit B (EC 2.8.3.12), at 3,325,438 to 3,326,217
_2836
Pf6N2E2_2837 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6); Glutaconate CoA-transferase subunit A (EC 2.8.3.12), at 3,326,214 to 3,327,074
_2837
Position (kb)
3324
3325
3326 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3323.293 kb on + strand, within Pf6N2E2_2833 at 3323.293 kb on + strand, within Pf6N2E2_2833 at 3323.293 kb on + strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.449 kb on + strand at 3323.449 kb on + strand at 3323.450 kb on - strand at 3323.450 kb on - strand at 3323.453 kb on - strand at 3323.453 kb on - strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.558 kb on + strand at 3323.672 kb on + strand, within Pf6N2E2_2834 at 3323.672 kb on + strand, within Pf6N2E2_2834 at 3323.672 kb on + strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.885 kb on + strand, within Pf6N2E2_2834 at 3323.885 kb on + strand, within Pf6N2E2_2834 at 3323.886 kb on - strand, within Pf6N2E2_2834 at 3324.146 kb on + strand at 3324.146 kb on + strand at 3324.218 kb on + strand at 3324.218 kb on + strand at 3324.219 kb on - strand at 3324.219 kb on - strand at 3324.221 kb on + strand at 3324.221 kb on + strand at 3324.221 kb on + strand at 3324.222 kb on - strand at 3324.224 kb on + strand at 3324.224 kb on + strand at 3324.225 kb on - strand at 3324.225 kb on - strand at 3324.225 kb on - strand at 3324.919 kb on - strand, within Pf6N2E2_2835 at 3324.919 kb on - strand, within Pf6N2E2_2835 at 3324.935 kb on - strand, within Pf6N2E2_2835 at 3324.966 kb on + strand, within Pf6N2E2_2835 at 3325.423 kb on + strand at 3325.423 kb on + strand at 3325.423 kb on + strand at 3325.423 kb on + strand at 3325.423 kb on + strand at 3325.423 kb on + strand at 3325.423 kb on + strand at 3325.424 kb on - strand at 3325.424 kb on - strand at 3325.424 kb on - strand at 3325.424 kb on - strand at 3325.425 kb on + strand at 3325.425 kb on + strand at 3325.425 kb on + strand at 3325.425 kb on + strand at 3325.426 kb on - strand at 3325.426 kb on - strand at 3325.426 kb on - strand at 3325.511 kb on - strand at 3325.668 kb on + strand, within Pf6N2E2_2836 at 3325.868 kb on + strand, within Pf6N2E2_2836 at 3325.869 kb on - strand, within Pf6N2E2_2836 at 3325.869 kb on - strand, within Pf6N2E2_2836 at 3326.038 kb on + strand, within Pf6N2E2_2836 at 3326.039 kb on - strand, within Pf6N2E2_2836 at 3326.039 kb on - strand, within Pf6N2E2_2836 at 3326.072 kb on + strand, within Pf6N2E2_2836 at 3326.073 kb on - strand, within Pf6N2E2_2836 at 3326.073 kb on - strand, within Pf6N2E2_2836 at 3326.073 kb on - strand, within Pf6N2E2_2836 at 3326.152 kb on + strand at 3326.152 kb on + strand at 3326.210 kb on + strand at 3326.211 kb on - strand at 3326.211 kb on - strand at 3326.211 kb on - strand at 3326.284 kb on + strand at 3326.284 kb on + strand at 3326.284 kb on + strand at 3326.285 kb on - strand at 3326.285 kb on - strand at 3326.344 kb on + strand, within Pf6N2E2_2837 at 3326.344 kb on + strand, within Pf6N2E2_2837 at 3326.344 kb on + strand, within Pf6N2E2_2837 at 3326.345 kb on - strand, within Pf6N2E2_2837 at 3326.369 kb on - strand, within Pf6N2E2_2837 at 3326.380 kb on + strand, within Pf6N2E2_2837 at 3326.380 kb on + strand, within Pf6N2E2_2837 at 3326.381 kb on - strand, within Pf6N2E2_2837
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,323,293 + Pf6N2E2_2833 0.65 -0.2 3,323,293 + Pf6N2E2_2833 0.65 +0.1 3,323,293 + Pf6N2E2_2833 0.65 -1.4 3,323,294 - Pf6N2E2_2833 0.65 +2.6 3,323,294 - Pf6N2E2_2833 0.65 +1.3 3,323,294 - Pf6N2E2_2833 0.65 -0.1 3,323,294 - Pf6N2E2_2833 0.65 +0.5 3,323,449 + +0.5 3,323,449 + +0.4 3,323,450 - -0.8 3,323,450 - -0.5 3,323,453 - +0.1 3,323,453 - +1.1 3,323,495 + +0.2 3,323,495 + +2.1 3,323,495 + +0.7 3,323,495 + +0.1 3,323,495 + -0.3 3,323,496 - +1.1 3,323,496 - -0.1 3,323,496 - +1.1 3,323,496 - +0.9 3,323,496 - +0.9 3,323,496 - +1.9 3,323,496 - -0.0 3,323,558 + +0.1 3,323,672 + Pf6N2E2_2834 0.25 -0.5 3,323,672 + Pf6N2E2_2834 0.25 -0.8 3,323,672 + Pf6N2E2_2834 0.25 +0.4 3,323,673 - Pf6N2E2_2834 0.25 -0.1 3,323,673 - Pf6N2E2_2834 0.25 +0.1 3,323,673 - Pf6N2E2_2834 0.25 +0.4 3,323,673 - Pf6N2E2_2834 0.25 +0.2 3,323,673 - Pf6N2E2_2834 0.25 +0.2 3,323,885 + Pf6N2E2_2834 0.55 -0.5 3,323,885 + Pf6N2E2_2834 0.55 +1.1 3,323,886 - Pf6N2E2_2834 0.56 -1.4 3,324,146 + -0.5 3,324,146 + +0.1 3,324,218 + +1.6 3,324,218 + +0.1 3,324,219 - +1.1 3,324,219 - +0.9 3,324,221 + +0.6 3,324,221 + +0.9 3,324,221 + -1.6 3,324,222 - -0.6 3,324,224 + +1.3 3,324,224 + -0.5 3,324,225 - -0.6 3,324,225 - -0.1 3,324,225 - -0.2 3,324,919 - Pf6N2E2_2835 0.57 +0.1 3,324,919 - Pf6N2E2_2835 0.57 -0.2 3,324,935 - Pf6N2E2_2835 0.58 -0.3 3,324,966 + Pf6N2E2_2835 0.61 -1.5 3,325,423 + +0.7 3,325,423 + -1.4 3,325,423 + +0.3 3,325,423 + -0.4 3,325,423 + -0.2 3,325,423 + +0.1 3,325,423 + -2.4 3,325,424 - +0.3 3,325,424 - +0.1 3,325,424 - +0.6 3,325,424 - +0.4 3,325,425 + +0.7 3,325,425 + +1.6 3,325,425 + +0.9 3,325,425 + -0.1 3,325,426 - -0.1 3,325,426 - +0.5 3,325,426 - +0.4 3,325,511 - +0.8 3,325,668 + Pf6N2E2_2836 0.29 +0.9 3,325,868 + Pf6N2E2_2836 0.55 +0.1 3,325,869 - Pf6N2E2_2836 0.55 -0.3 3,325,869 - Pf6N2E2_2836 0.55 +0.9 3,326,038 + Pf6N2E2_2836 0.77 +0.9 3,326,039 - Pf6N2E2_2836 0.77 -2.3 3,326,039 - Pf6N2E2_2836 0.77 -0.9 3,326,072 + Pf6N2E2_2836 0.81 -0.0 3,326,073 - Pf6N2E2_2836 0.81 -0.2 3,326,073 - Pf6N2E2_2836 0.81 +0.4 3,326,073 - Pf6N2E2_2836 0.81 -0.5 3,326,152 + -0.5 3,326,152 + -0.8 3,326,210 + +0.9 3,326,211 - -0.2 3,326,211 - -0.5 3,326,211 - -0.9 3,326,284 + +2.2 3,326,284 + +0.5 3,326,284 + -0.3 3,326,285 - +0.1 3,326,285 - +0.7 3,326,344 + Pf6N2E2_2837 0.15 -1.5 3,326,344 + Pf6N2E2_2837 0.15 +0.1 3,326,344 + Pf6N2E2_2837 0.15 +0.5 3,326,345 - Pf6N2E2_2837 0.15 +0.4 3,326,369 - Pf6N2E2_2837 0.18 +0.2 3,326,380 + Pf6N2E2_2837 0.19 -0.5 3,326,380 + Pf6N2E2_2837 0.19 +0.3 3,326,381 - Pf6N2E2_2837 0.19 +1.1
Or see this region's nucleotide sequence